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Literature summary extracted from

  • Xian, L.; Wang, F.; Yin, X.; Feng, J.X.
    Identification and characterization of an acidic and acid-stable endoxyloglucanase from Penicillium oxalicum (2016), Int. J. Biol. Macromol., 86, 512-518 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.151 additional information no effect on enzyme activity by 1-5 mM EDTA Penicillium oxalicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.151 gene PoxXEG12A, sequence comparisons, recombinant expression of His-tagged enzyme in Pichia pastoris strain GS115 under control of methanol-inducible AOX1 promoter Penicillium oxalicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.151 Ca2+ slight inhibition at 1-5 mM Penicillium oxalicum
3.2.1.151 Co2+ slight inhibition at 1-5 mM Penicillium oxalicum
3.2.1.151 Cr2+ slight inhibition at 1-5 mM Penicillium oxalicum
3.2.1.151 Cu2+ 65% inhibition at 5 mM Penicillium oxalicum
3.2.1.151 Fe2+ slight inhibition at 1-5 mM Penicillium oxalicum
3.2.1.151 Fe3+ slight inhibition at 1-5 mM Penicillium oxalicum
3.2.1.151 K+ slight inhibition at 1-5 mM Penicillium oxalicum
3.2.1.151 Li+ slight inhibition at 1-5 mM Penicillium oxalicum
3.2.1.151 Mg2+ slight inhibition at 1-5 mM Penicillium oxalicum
3.2.1.151 additional information no effect on enzyme activity by 1-5 mM EDTA Penicillium oxalicum
3.2.1.151 Na+ slight inhibition at 1-5 mM Penicillium oxalicum
3.2.1.151 Ni2+ slight inhibition at 1-5 mM Penicillium oxalicum
3.2.1.151 Zn2+ slight inhibition at 1-5 mM Penicillium oxalicum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.151 Penicillium oxalicum A0A0A7RRF2
-
-
3.2.1.151 Penicillium oxalicum HP7-1 A0A0A7RRF2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.151 recombinant His-tagged enzyme from Pichia pastoris strain GS115 by nickel affinity chromatography Penicillium oxalicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.151 172
-
 recombinant enzyme, substrate tamarind xyloglucan, pH 5.0, 60°C Penicillium oxalicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.151 additional information substrate specificity, the enzyme is highly specific for tamarind xyloglucan, it shows no activity against lichenan, methylcellulose, carboxymethyl cellulose, Avicel, xylan from beechwood, xylan from birch wood, barley glucan, laminarin, and galactan as substrates Penicillium oxalicum ?
-
 ?
3.2.1.151 additional information substrate specificity, the enzyme is highly specific for tamarind xyloglucan, it shows no activity against lichenan, methylcellulose, carboxymethyl cellulose, Avicel, xylan from beechwood, xylan from birch wood, barley glucan, laminarin, and galactan as substrates Penicillium oxalicum HP7-1 ?
-
 ?
3.2.1.151 tamarind seed xyloglucan + H2O the enzyme specifically hydrolyzes tamarind xyloglucan in endo-acting mode Penicillium oxalicum xyloglucooligosaccharides
-
 ?
3.2.1.151 tamarind seed xyloglucan + H2O the enzyme specifically hydrolyzes tamarind xyloglucan in endo-acting mode Penicillium oxalicum HP7-1 xyloglucooligosaccharides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.151 endoxyloglucanase
-
 Penicillium oxalicum
3.2.1.151 POXEG12A gene name, UniProt Penicillium oxalicum
3.2.1.151 PoxXEG12A
-
 Penicillium oxalicum
3.2.1.151 xyloglucanase
-
 Penicillium oxalicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.151 55 60
-
 Penicillium oxalicum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.151 30 70 activity range, profile overview. The enzyme shows over 50% of maximal activity at 45-65°C Penicillium oxalicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.151 40
-
 recombinant enzyme, pH 5.0, completely stable up to Penicillium oxalicum
3.2.1.151 45 50 recombinant enzyme, pH 5.0, 40% activity remaining, inactivation at 50°C Penicillium oxalicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.151 4.5 5.5
-
 Penicillium oxalicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.151 3 8.5 recombinant enzyme, activity range, profile overview. The enzyme shows over 90% of maximal activity at pH 3.5-7.0 Penicillium oxalicum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.151 3.5 7 recombinant enzyme, 37°C, stable at Penicillium oxalicum