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Literature summary extracted from

  • Sun, Z.; Liu, P.; Cheng, G.; Zhang, B.; Dong, W.; Su, X.; Huang, Y.; Cui, Z.; Kong, Y.
    A fibrinolytic protease AfeE from Streptomyces sp. CC5, with potent thrombolytic activity in a mouse model (2016), Int. J. Biol. Macromol., 85, 346-354 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.24.72 medicine the enzyme is a candidate for antithrombotic drug development Streptomyces sp. CC5

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.72 aprotin 84.1% residual activity at 1 mM Streptomyces sp. CC5
3.4.24.72 Co2+ 60.4% residual activity at 1 mM Streptomyces sp. CC5
3.4.24.72 Cu2+ 50.1% residual activity at 1 mM Streptomyces sp. CC5
3.4.24.72 EDTA 77.5% residual activity at 5 mM Streptomyces sp. CC5
3.4.24.72 EGTA 74.7% residual activity at 5 mM Streptomyces sp. CC5
3.4.24.72 Fe2+ 77.3% residual activity at 1 mM Streptomyces sp. CC5
3.4.24.72 leupeptin 98.6% residual activity at 1 mM Streptomyces sp. CC5
3.4.24.72 Mn2+ 94.2% residual activity at 1 mM Streptomyces sp. CC5
3.4.24.72 Na+ 97.7% residual activity at 1 mM Streptomyces sp. CC5
3.4.24.72 pepstatin A 95.2% residual activity at 1 mM Streptomyces sp. CC5
3.4.24.72 phenylmethylsulfonyl fluoride 18.5% residual activity at 1 mM Streptomyces sp. CC5
3.4.24.72 Soybean trypsin inhibitor 28.2% residual activity at 1 mM Streptomyces sp. CC5
3.4.24.72 tosyl-L-lysine chloromethyl ketone 21.3% residual activity at 0.1 mM Streptomyces sp. CC5
3.4.24.72 tosyl-L-phenylalanine chloromethyl ketone 37.2% residual activity at 0.1 mM Streptomyces sp. CC5
3.4.24.72 Zn2+ 67.6% residual activity at 1 mM Streptomyces sp. CC5

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.72 Ca2+ 108.7% activity at 1 mM Streptomyces sp. CC5
3.4.24.72 Mg2+ 112.6% activity at 1 mM Streptomyces sp. CC5
3.4.24.72 additional information not influenced by K+ Streptomyces sp. CC5

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.72 Streptomyces sp. CC5 A0A0A7W604
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.72 ammonium sulfate precipitation, HPD-100 macroporous resin column chromatography, 201 × 7 ion-exchange resin column chromatography, and Superdex 200 gel filtration Streptomyces sp. CC5

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.72 albumin from chicken egg + H2O lowest activity Streptomyces sp. CC5 ?
-
 ?
3.4.24.72 Bovine serum albumin + H2O
-
 Streptomyces sp. CC5 ?
-
 ?
3.4.24.72 D-Ile-Pro-Arg-4-nitroanilide + H2O
-
 Streptomyces sp. CC5 D-Ile-Pro-Arg + 4-nitroaniline
-
 ?
3.4.24.72 D-Phe-Pip-Arg-4-nitroanilide + H2O
-
 Streptomyces sp. CC5 D-Phe-Pip-Arg + 4-nitroaniline
-
 ?
3.4.24.72 D-Val-Leu-Lys-4-nitroanilide + H2O
-
 Streptomyces sp. CC5 D-Val-Leu-Lys + 4-nitroaniline
-
 ?
3.4.24.72 Fibrin + H2O best substrate. The enzyme exhibits about 2fold higher activity for fibrin than fibrinogen. Fibrin can be hydrolyzed completely in about 30 min Streptomyces sp. CC5 ?
-
 ?
3.4.24.72 fibrinogen + H2O fibrinogen can be hydrolyzed completely in 4-6 h Streptomyces sp. CC5 fibrin + ?
-
 ?
3.4.24.72 Hemoglobin + H2O
-
 Streptomyces sp. CC5 ?
-
 ?
3.4.24.72 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O second best substrate Streptomyces sp. CC5 N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.72 ? x * 29984, high resolution mass spectrometry Streptomyces sp. CC5

Synonyms

EC Number Synonyms Comment Organism
3.4.24.72 AfeE
-
 Streptomyces sp. CC5
3.4.24.72 fibrinolytic protease
-
 Streptomyces sp. CC5

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.72 57
-
-
 Streptomyces sp. CC5

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.24.72 37 60 the enzyme maintains up to 80% of its maximum activity at a temperature range of 37-60°C Streptomyces sp. CC5

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.72 20 70 the enzyme activity remains stable for 60 min at 20-30°C and loses about 50% activity after 60 min at 50°C. After 60 min at 60°C, the enzyme completely loses its activity Streptomyces sp. CC5

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.72 6 12 the enzyme shows more than 60% activity at pH values ranging from 6.0 to 12.0 Streptomyces sp. CC5

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.72 6 12 the enzyme activity remains stable after 60 min at pH 6.0-12.0 Streptomyces sp. CC5