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Literature summary extracted from

  • Homaei, A.
    Enhanced activity and stability of papain immobilized on CNBr-activated sepharose (2015), Int. J. Biol. Macromol., 75, 373-377 .
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.22.2 immobilization of papain by covalent attachment on Sepharose 6B activated by using cyanogen bromide brings about significant enhancement of storage and thermal stability and of stability at extreme pHs Carica papaya

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.2 Ba2+ 5 mM, 24% loss of activity (soluble enzyme), 9% loss of activity (enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide) Carica papaya
3.4.22.2 Ca2+ 5 mM, 22% loss of activity (soluble enzyme), 6% loss of activity (enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide) Carica papaya
3.4.22.2 Co2+ 5 mM, 46% loss of activity (soluble enzyme), 30% loss of activity (enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide) Carica papaya
3.4.22.2 Fe2+ 5 mM, 44% loss of activity (soluble enzyme), 32% loss of activity (enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide) Carica papaya
3.4.22.2 Mg2+ 5 mM, 19% loss of activity (soluble enzyme), 4% loss of activity (enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide) Carica papaya
3.4.22.2 Mn2+ 5 mM, 44% loss of activity (soluble enzyme), 31% loss of activity (enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide) Carica papaya
3.4.22.2 additional information immobilization of papain by covalent attachment on Sepharose 6B activated by using cyanogen bromide brings about resistance against the inhibitory effects of various bivalent metal ions with respect to papain Carica papaya
3.4.22.2 Zn2+ 5 mM, 51% loss of activity (soluble enzyme), 45% loss of activity (enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide) Carica papaya

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.2 0.00062
-
 casein soluble enzyme, pH 7.5, 23°C Carica papaya
3.4.22.2 0.00079
-
 casein enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide, pH 7.5, 23°C Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya P00784
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.2 0.205
-
 substrate: casein, enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide, pH 7.5, 23°C Carica papaya
3.4.22.2 0.232
-
 substrate: casein, soluble enzyme, pH 7.5, 23°C Carica papaya

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 casein + H2O
-
 Carica papaya ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.2 60
-
 soluble enzyme Carica papaya
3.4.22.2 80
-
 enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide Carica papaya

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.2 80
-
 half-life: 18 min (soluble enzyme). Immobilized papain retains 80% of its original activity after 1 h incubation Carica papaya
3.4.22.2 90
-
 half-life: 6 min (soluble enzyme), 40 min (enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide). Immobilized papain retains 45% of its original activity after 1 h incubation Carica papaya

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.2 0.0102
-
 casein enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide, pH 7.5, 23°C Carica papaya
3.4.22.2 0.0162
-
 casein soluble enzyme, pH 7.5, 23°C Carica papaya

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.2 6.5
-
 soluble enzyme Carica papaya
3.4.22.2 8
-
 enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide Carica papaya