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Literature summary extracted from
- Does macromolecular crowding compatible with enzyme stem bromelain structure and stability? (2019), Int. J. Biol. Macromol., 131, 527-535 .
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.22.32 | dextran (D70) and polyethylene glycol (P12 and P20) destablize native bromelain structure | Ananas comosus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.32 | Dextran | dextran 70 kDa, for dextran, the crowding effect clearly depends on its molecular weight | Ananas comosus |  |
| 3.4.22.32 | additional information | crowders, such as dextran (D70) and polyethylene glycol (P12 and P20), destablize and deactivate native bromelain, absorption spectra in phosphate buffer, overview. PEGs tend to have different effects on different proteins depending upon the chemical nature of proteins, while for dextran, the crowding effect clearly depends on its molecular weight. PEG does not seem to follow the size-dependent crowding effect as in the case of dextran | Ananas comosus | |
| 3.4.22.32 | polyethylene glycol 12000 | - |
Ananas comosus | |
| 3.4.22.32 | polyethylene glycol 20000 | - |
Ananas comosus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.32 | Ananas comosus | P14518 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.22.32 | stem | - |
Ananas comosus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.32 | casein + H2O | denatured casein | Ananas comosus | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.32 | 7 | - |
assay at | Ananas comosus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.4.22.32 | additional information | analysis of of macromolecular crowding on stem bromelain, i.e. effects of dextran (D70) and polyethylene glycol (P12 and P20) on native bromelain structure, by combining the results of absorption, circular dichroism, fluorescence and activity studies, detailed overview. The overall effect of the crowders on the enzyme is destabilization and deactivation. Molecular docking simulation of bromelain with PEG and dextran | Ananas comosus |
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Release 2023.1 (January 2023)
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