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Literature summary extracted from

  • Bhat, S.Y.; Dey, A.; Qureshi, I.A.
    Structural and functional highlights of methionine aminopeptidase 2 from Leishmania donovani (2018), Int. J. Biol. Macromol., 115, 940-954 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.18 expression in Escherichia coli Leishmania donovani

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.11.18 homology modelling of MAP2 and molecular docking studies Leishmania donovani

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.18 bestatin competitive Leishmania donovani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.18 0.125
-
 L-alanine-4-methylcoumarin-7-amide presence of Ca2+, pH 8.0, 37°C Leishmania donovani
3.4.11.18 0.154
-
 L-alanine-4-methylcoumarin-7-amide presence of Co2+, pH 8.0, 37°C Leishmania donovani
3.4.11.18 0.167
-
 L-alanine-4-methylcoumarin-7-amide presence of Mn2+, pH 8.0, 37°C Leishmania donovani
3.4.11.18 0.172
-
 L-alanine-4-methylcoumarin-7-amide presence of Cu2+, pH 8.0, 37°C Leishmania donovani
3.4.11.18 0.179
-
 L-alanine-4-methylcoumarin-7-amide presence of Mg2+, pH 8.0, 37°C Leishmania donovani
3.4.11.18 0.284
-
 L-alanine-4-methylcoumarin-7-amide presence of Ni2+, pH 8.0, 37°C Leishmania donovani

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.18 Ca2+ highest activtiy in presence of Co2+ or Ca2+ Leishmania donovani
3.4.11.18 Co2+ highest activtiy in presence of Co2+ or Ca2+ Leishmania donovani
3.4.11.18 Cu2+
-
 Leishmania donovani
3.4.11.18 Mg2+
-
 Leishmania donovani
3.4.11.18 Mn2+
-
 Leishmania donovani
3.4.11.18 additional information Zn(II)-activated MAP2 shows negligible amidolytic activity Leishmania donovani
3.4.11.18 Ni2+
-
 Leishmania donovani

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.18 Leishmania donovani A0A3S7WWI2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.18 L-alanine-4-methylcoumarin-7-amide + H2O
-
 Leishmania donovani L-alanine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.18 L-leucine-4-methylcoumarin-7-amide + H2O
-
 Leishmania donovani L-leucine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.18 L-methionine-4-methylcoumarin-7-amide + H2O
-
 Leishmania donovani L-methionine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.18 additional information among the 4-methylcoumarin-7-amides tested, activity is highest with L-alanine 4-methylcoumarin-7-amide Leishmania donovani ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.18 ? x * 51300, calculated from sequence Leishmania donovani

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.18 0.43
-
 L-alanine-4-methylcoumarin-7-amide presence of Cu2+, pH 8.0, 37°C Leishmania donovani
3.4.11.18 1.47
-
 L-alanine-4-methylcoumarin-7-amide presence of Ni2+, pH 8.0, 37°C Leishmania donovani
3.4.11.18 1.5
-
 L-alanine-4-methylcoumarin-7-amide presence of Ca2+, pH 8.0, 37°C Leishmania donovani
3.4.11.18 1.66
-
 L-alanine-4-methylcoumarin-7-amide presence of Mn2+, pH 8.0, 37°C Leishmania donovani
3.4.11.18 1.82
-
 L-alanine-4-methylcoumarin-7-amide presence of Mg2+, pH 8.0, 37°C Leishmania donovani
3.4.11.18 2.13
-
 L-alanine-4-methylcoumarin-7-amide presence of Co2+, pH 8.0, 37°C Leishmania donovani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.18 7.5
-
-
 Leishmania donovani

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.18 0.086
-
 bestatin pH 8.0, 37°C Leishmania donovani

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.11.18 Leishmania donovani calculated from sequence
-
 6.6

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.11.18 2.5
-
 L-alanine-4-methylcoumarin-7-amide presence of Cu2+, pH 8.0, 37°C Leishmania donovani
3.4.11.18 5.17
-
 L-alanine-4-methylcoumarin-7-amide presence of Ni2+, pH 8.0, 37°C Leishmania donovani
3.4.11.18 9.8
-
 L-alanine-4-methylcoumarin-7-amide presence of Mn2+, pH 8.0, 37°C Leishmania donovani
3.4.11.18 10
-
 L-alanine-4-methylcoumarin-7-amide presence of Mg2+, pH 8.0, 37°C Leishmania donovani
3.4.11.18 12
-
 L-alanine-4-methylcoumarin-7-amide presence of Ca2+, pH 8.0, 37°C Leishmania donovani
3.4.11.18 13.83
-
 L-alanine-4-methylcoumarin-7-amide presence of Co2+, pH 8.0, 37°C Leishmania donovani