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Literature summary extracted from

  • Zhan, W.; Jin, L.; Jiao, J.; Zhang, X.; Zhang, Y.; Zhao, H.; Liang, M.
    Expression and purification of plant fructan exohydrolases and their potential applications in fructose production (2018), Int. J. Biol. Macromol., 108, 9-17 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.80 gene FEH-III, DNA and amino acid sequence determination and analysis, FEH isozyme sequences comparisons, phylogenetic analysis, functional recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33 Helianthus tuberosus
3.2.1.153 gene FEH-I, FEH isozyme sequences comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33 Cichorium intybus
3.2.1.153 gene FEH-IIa, FEH isozyme sequences comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33 Cichorium intybus
3.2.1.153 gene FEH-IIb, FEH isozyme sequences comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33 Cichorium intybus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.153 additional information immobilization of purified recombinant isozyme Ci1-FEH IIa Cichorium intybus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.80 Helianthus tuberosus
-
-
-
3.2.1.153 Cichorium intybus Q93X59
-
-
3.2.1.153 Cichorium intybus Q93X60
-
-
3.2.1.153 Cichorium intybus Q9FNS9
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.80 glycoprotein the Ht1-FEH III sequence harbors four potential N-glycosylation sites Helianthus tuberosus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.80 recombinant His-tagged enzyme from Pichia pastoris strain X-33 by nickel affinity chromatography and ultrafiltration Helianthus tuberosus
3.2.1.153 recombinant His-tagged enzyme from Pichia pastoris strain X-33 by nickel affinity chromatography and ultrafiltration Cichorium intybus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.80 1-kestose + H2O best substrate Helianthus tuberosus beta-D-fructose + sucrose
-
 ?
3.2.1.80 fructofuranosylnystose + H2O high activity Helianthus tuberosus ?
-
 ?
3.2.1.80 inulin + H2O high activity Helianthus tuberosus ?
-
 ?
3.2.1.80 levan + H2O low to moderate activity Helianthus tuberosus ?
-
 ?
3.2.1.80 additional information substrate specificity, overview Helianthus tuberosus ?
-
 ?
3.2.1.80 nystose + H2O
-
 Helianthus tuberosus ?
-
 ?
3.2.1.80 sucrose + H2O
-
 Helianthus tuberosus D-glucose + D-fructose
-
 ?
3.2.1.153 1-kestose + H2O
-
 Cichorium intybus beta-D-fructose + sucrose
-
 ?
3.2.1.153 1-kestose + H2O best substrate Cichorium intybus beta-D-fructose + sucrose
-
 ?
3.2.1.153 fructofuranosylnystose + H2O
-
 Cichorium intybus ?
-
 ?
3.2.1.153 fructofuranosylnystose + H2O best substrate Cichorium intybus ?
-
 ?
3.2.1.153 fructofuranosylnystose + H2O high activity Cichorium intybus ?
-
 ?
3.2.1.153 inulin + H2O best substrate Cichorium intybus ?
-
 ?
3.2.1.153 inulin + H2O high activity Cichorium intybus ?
-
 ?
3.2.1.153 additional information hydrolysis of Jerusalem artichoke tuber by immobilized isozyme Ci1-FEH IIa and analysis of carbohydrate profile. The fructan in the tuber is rapidly degraded to fructose by immobilized recombinant Ci1-FEH IIa. Substrate specificity, overview. No activity with sucrose and levan. Isozyme Ci1-FEH IIa has much higher activity than all other investigated FEH isozymes from Cichorium intybus and Helianthus tuberosus Cichorium intybus ?
-
 ?
3.2.1.153 additional information substrate specificity, overview. No activity with sucrose and levan Cichorium intybus ?
-
 ?
3.2.1.153 additional information substrate specificity, overview. No activity with sucrose and levan. Isozyme Ci1-FEH I shows quite low activity compared to other FEH isozymes analyzed from Cichorium intybus and Helianthus Cichorium intybus ?
-
 ?
3.2.1.153 nystose + H2O
-
 Cichorium intybus ?
-
 ?
3.2.1.153 nystose + H2O best substrate Cichorium intybus ?
-
 ?
3.2.1.153 nystose + H2O high activity Cichorium intybus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.80 ? x * 64700, recombinant His-tagged enzyme, SDS-PAGE Helianthus tuberosus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.80 Ht1-FEH III
-
 Helianthus tuberosus
3.2.1.153 Ci1-FEH I
-
 Cichorium intybus
3.2.1.153 Ci1-FEH IIa
-
 Cichorium intybus
3.2.1.153 Ci1-FEH IIb
-
 Cichorium intybus
3.2.1.153 fructan 1-exohydrolase I
-
 Cichorium intybus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.80 55
-
-
 Helianthus tuberosus
3.2.1.153 35
-
-
 Cichorium intybus
3.2.1.153 40 50
-
 Cichorium intybus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.80 35 60 over 50% of maximal activity within this range Helianthus tuberosus
3.2.1.153 20 50 over 60% of maximal activity within this range Cichorium intybus
3.2.1.153 20 70 over 60% of maximal activity within this range Cichorium intybus
3.2.1.153 25 45 over 50% of maximal activity within this range Cichorium intybus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.80 5
-
-
 Helianthus tuberosus
3.2.1.153 5.5
-
-
 Cichorium intybus
3.2.1.153 6
-
-
 Cichorium intybus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.80 4 6.5 over 60% of maximal activity within this range Helianthus tuberosus
3.2.1.153 4 6 over 80% of maximal activity within this range Cichorium intybus
3.2.1.153 4 6.5 over 50% of maximal activity within this range Cichorium intybus
3.2.1.153 4 7 over 40% of maximal activity within this range Cichorium intybus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.80 Helianthus tuberosus sequence calculation
-
 6.45

General Information

EC Number General Information Comment Organism
3.2.1.80 evolution phylogenetic analysis of deduced amino acid sequences of plant fructan metabolism genes Helianthus tuberosus
3.2.1.80 additional information the Ht1-FEH III sequence harbors three conserved amino acid motifs (S/NDPNG, FRDP, and ECPD) and four potential N-glycosylation sites Helianthus tuberosus
3.2.1.80 physiological function the third member of the Ht1-FEH family in Jerusalem artichoke, i.e., Ht1-FEH III, not only demonstrates hydrolysis activity towards beta-(2->1)-linked fructans and beta-(2->6)-linked levan, but also towards sucrose Helianthus tuberosus
3.2.1.153 evolution phylogenetic analysis of deduced amino acid sequences of plant fructan metabolism genes Cichorium intybus