Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Maharem, T.M.; Zahran, W.E.; Hassan, R.E.; Abdel Fattah, M.M.
    Unique properties of arginase purified from camel liver cytosol (2018), Int. J. Biol. Macromol., 108, 88-97 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.1 DL-isoleucine competitive inhibitor Camelus dromedarius
3.5.3.1 L-leucine competitive inhibitor Camelus dromedarius
3.5.3.1 L-lysine competitive inhibitor Camelus dromedarius
3.5.3.1 L-ornithine competitive inhibitor Camelus dromedarius
3.5.3.1 L-valine competitive inhibitor Camelus dromedarius
3.5.3.1 Sr2+ potent inhibitor Camelus dromedarius
3.5.3.1 Zn2+ potent inhibitor Camelus dromedarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.1 7.1
-
 L-arginine pH 7.5, 37°C Camelus dromedarius

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.1 Co2+ the enzyme retains 38.7% of its original activity after dialysis. In the presence of Mn2+ as a cofactor, the enzyme regains 95% of its initial activity Camelus dromedarius
3.5.3.1 Fe3+ the enzyme retains 38.7% of its original activity after dialysis. In the presence of Mn2+ as a cofactor, the enzyme regains 95% of its initial activity Camelus dromedarius
3.5.3.1 Mn2+ the enzyme retains 38.7% of its original activity after dialysis. In the presence of Mn2+ as a cofactor, the enzyme regains 96% of its initial activity Camelus dromedarius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.1 180000
-
-
 Camelus dromedarius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.1 L-arginine + H2O Camelus dromedarius the enzyme involved in urea cycle L-ornithine + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.1 Camelus dromedarius
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.1
-
 Camelus dromedarius

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.3.1 liver
-
 Camelus dromedarius
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.1 L-arginine + H2O
-
 Camelus dromedarius L-ornithine + urea
-
 ?
3.5.3.1 L-arginine + H2O the enzyme involved in urea cycle Camelus dromedarius L-ornithine + urea
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.1 ? x * 35000, SDS-PAGE Camelus dromedarius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.1 70
-
-
 Camelus dromedarius

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.3.1 45 80 45°C: about 45% of maximal activity, 80°C: about 75% of maximal activity Camelus dromedarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.1 9
-
 activity as a function of pH increases as the pH increases up to pH 9 and pH 10.7 of 50 mM Tris-HCl buffer and 50 mM carbonate bicarbonate buffer Camelus dromedarius
3.5.3.1 10.7
-
 activity as a function of pH increases as the pH increases up to pH 9 and pH 10.7 of 50 mM Tris-HCl buffer and 50 mM carbonate bicarbonate buffer Camelus dromedarius

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.3.1 5
-
 L-valine pH 7.5, 37°C Camelus dromedarius
3.5.3.1 8
-
 L-lysine pH 7.5, 37°C Camelus dromedarius
3.5.3.1 10
-
 L-leucine pH 7.5, 37°C Camelus dromedarius
3.5.3.1 17.5
-
 DL-isoleucine pH 7.5, 37°C Camelus dromedarius
3.5.3.1 30
-
 L-ornithine pH 7.5, 37°C Camelus dromedarius

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.3.1 Camelus dromedarius isoelectric focusing
-
 7.7

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.3.1 9
-
 pH 7.5, 37°C Camelus dromedarius L-leucine
3.5.3.1 9.5
-
 pH 7.5, 37°C Camelus dromedarius DL-isoleucine
3.5.3.1 13
-
 pH 7.5, 37°C Camelus dromedarius L-valine
3.5.3.1 22
-
 pH 7.5, 37°C Camelus dromedarius L-lysine
3.5.3.1 24
-
 pH 7.5, 37°C Camelus dromedarius L-ornithine

General Information

EC Number General Information Comment Organism
3.5.3.1 metabolism the enzyme involved in urea cycle Camelus dromedarius