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Literature summary extracted from

  • Jang, S.; Kang, C.; Yang, H.S.; Jung, T.; Hebert, H.; Chung, K.Y.; Kim, S.J.; Hohng, S.; Song, J.J.
    Structural basis of recognition and destabilization of the histone H2B ubiquitinated nucleosome by the DOT1L histone H3 Lys79 methyltransferase (2019), Genes Dev., 33, 620-625 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.355 additional information the enzyme activity is stimulated by histone H2B Lys120 ubiquitination Homo sapiens
2.1.1.360 histone H2B ubiquitin the enzyme activity is stimulated by histone H2B Lys120 ubiquitination Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.360 expressed in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.355 F326A the mutant shows significantly reduced activity compared to the wild type enzyme Homo sapiens
2.1.1.355 F326A the mutation slightly decreases the enzyme activity Homo sapiens
2.1.1.355 I290A the mutant shows significantly reduced activity compared to the wild type enzyme Homo sapiens
2.1.1.355 L284A the mutant shows significantly reduced activity compared to the wild type enzyme Homo sapiens
2.1.1.355 L329A the mutant shows significantly reduced activity compared to the wild type enzyme Homo sapiens
2.1.1.355 R282A the mutation dramatically decreases the enzyme activity Homo sapiens
2.1.1.360 F326A the mutant shows significantly decreased enzyme activity compared to the wild type Homo sapiens
2.1.1.360 I290A the mutant shows significantly decreased enzyme activity compared to the wild type Homo sapiens
2.1.1.360 L284A the mutant shows significantly decreased enzyme activity compared to the wild type Homo sapiens
2.1.1.360 R282A the mutant shows dramatically decreased enzyme activity compared to the wild type Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.360 nucleus
-
Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.355 3 S-adenosyl-L-methionine + a [histone H3]-L-lysine9 Homo sapiens overall reaction 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine9
-
?
2.1.1.355 S-adenosyl-L-methionine + a [histone H3]-L-lysine9 Homo sapiens
-
S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine9
-
?
2.1.1.355 S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine9 Homo sapiens
-
S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine9
-
?
2.1.1.355 S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine9 Homo sapiens
-
S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine9
-
?
2.1.1.355 S-adenosyl-L-methionine + H2B ubiquitinated nucleosome Homo sapiens
-
?
-
?
2.1.1.360 3 S-adenosyl-L-methionine + a [histone H3]-L-lysine79 Homo sapiens overall reaction 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine79
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.355 Homo sapiens Q8TEK3
-
-
2.1.1.360 Homo sapiens Q8TEK3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.355 glutahione S-transferase affinity column chromatography, anion exchange column chromatography, and gel filtration Homo sapiens
2.1.1.360 glutahione S-transferase affinity column chromatography, cation exchange chromatography and S200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.355 3 S-adenosyl-L-methionine + a [histone H3]-L-lysine9 overall reaction Homo sapiens 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine9
-
?
2.1.1.355 S-adenosyl-L-methionine + a [histone H3]-L-lysine9
-
Homo sapiens S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine9
-
?
2.1.1.355 S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine9
-
Homo sapiens S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine9
-
?
2.1.1.355 S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine9
-
Homo sapiens S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine9
-
?
2.1.1.355 S-adenosyl-L-methionine + H2B ubiquitinated nucleosome
-
Homo sapiens ?
-
?
2.1.1.360 3 S-adenosyl-L-methionine + a [histone H3]-L-lysine79 overall reaction Homo sapiens 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine79
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.355 DOT1L
-
Homo sapiens
2.1.1.355 histone H3 Lys79 methyltransferase
-
Homo sapiens
2.1.1.360 DOT1L
-
Homo sapiens
2.1.1.360 histone H3 Lys79 methyltransferase
-
Homo sapiens