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Literature summary extracted from
- ; Bastos, I.M.D.; Neves, D.; Kipnis, A.; Junqueira-Kipnis, A.P.; de Santana, J.M. The activity of a hexameric M17 metallo-aminopeptidase is associated with survival of Mycobacterium tuberculosis (2017), Front. Microbiol., 8, 504-516 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.11.10 | gene pepA, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL10-gold | Mycobacterium tuberculosis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.10 | 1,10-phenanthroline | 86.5% inhibition | Mycobacterium tuberculosis |  |
| 3.4.11.10 | bestatin | complete enzyme inhibition at 0.03 mM with substrate L-Leu-7-amido-4-methylcoumarin, in a murine model of tuberculosis, bestatin treatment reduces bacterial burden and lesion in the lungs of infected mice. Bestatin inhibits Mycobacterium tuberculosis growth in in vitro and ex vivo | Mycobacterium tuberculosis | |
| 3.4.11.10 | EDTA | 68% inhibition | Mycobacterium tuberculosis | |
| 3.4.11.10 | L-trans-epoxysuccinylleucylamido-4-guanidino butane | E-64 | Mycobacterium tuberculosis | |
| 3.4.11.10 | additional information | no inhibition by leupeptin and pepstatin A | Mycobacterium tuberculosis | |
| 3.4.11.10 | phenylmethylsulfonyl fluoride | PMSF | Mycobacterium tuberculosis | |
| 3.4.11.10 | tosyl phenylalanyl chloromethyl ketone | TPCK | Mycobacterium tuberculosis | |
| 3.4.11.10 | tosyl-lysylchloromethane | TLCK | Mycobacterium tuberculosis | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.11.10 | cytosol | - |
Mycobacterium tuberculosis | 5829 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.10 | Ni2+ | Mycobacterium tuberculosis LAP exhibits maximum activity in the presence of Ni2+, but it is active in the presence of a broad range of other metals | Mycobacterium tuberculosis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.10 | 323000 | - |
recombinant enzyme, gel filtration | Mycobacterium tuberculosis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.10 | Mycobacterium tuberculosis | P9WHT3 | - |
- |
| 3.4.11.10 | Mycobacterium tuberculosis H37Rv | P9WHT3 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.10 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.10 | L-Arg-7-amido-4-methylcoumarin + H2O | - |
Mycobacterium tuberculosis | L-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.10 | L-Arg-7-amido-4-methylcoumarin + H2O | - |
Mycobacterium tuberculosis H37Rv | L-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.10 | L-Asp-7-amido-4-methylcoumarin + H2O | - |
Mycobacterium tuberculosis | L-Asp + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.10 | L-Asp-7-amido-4-methylcoumarin + H2O | - |
Mycobacterium tuberculosis H37Rv | L-Asp + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.10 | L-Leu-7-amido-4-methylcoumarin + H2O | - |
Mycobacterium tuberculosis | L-Leu + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.10 | L-Leu-7-amido-4-methylcoumarin + H2O | - |
Mycobacterium tuberculosis H37Rv | L-Leu + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.10 | L-Met-7-amido-4-methylcoumarin + H2O | - |
Mycobacterium tuberculosis | L-Met + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.10 | L-Met-7-amido-4-methylcoumarin + H2O | - |
Mycobacterium tuberculosis H37Rv | L-Met + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.10 | L-Pro-7-amido-4-methylcoumarin + H2O | - |
Mycobacterium tuberculosis | L-Pro + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.10 | L-Pro-7-amido-4-methylcoumarin + H2O | - |
Mycobacterium tuberculosis H37Rv | L-Pro + 7-amino-4-methylcoumarin | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.11.10 | hexamer | 6 * 55000, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.11.10 | leucine aminopeptidase | - |
Mycobacterium tuberculosis |
| 3.4.11.10 | M17 metallo-aminopeptidase | - |
Mycobacterium tuberculosis |
| 3.4.11.10 | MtLAP | - |
Mycobacterium tuberculosis |
| 3.4.11.10 | PepA | - |
Mycobacterium tuberculosis |
| 3.4.11.10 | Rv2213 | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.10 | 25 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.10 | 7.5 | - |
assay at | Mycobacterium tuberculosis |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.4.11.10 | evolution | sequence analysis and phylogenetic relationship of M17 LAPs | Mycobacterium tuberculosis |
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Release 2023.1 (January 2023)
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