EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.24 | acetone | slight activation | Lactobacillus gasseri | |
3.5.1.24 | butanol | - |
Lactobacillus gasseri | |
3.5.1.24 | DTT | slight activation at 10 mM | Lactobacillus gasseri | |
3.5.1.24 | Isopropanol | - |
Lactobacillus gasseri | |
3.5.1.24 | Triton X-100 | - |
Lactobacillus gasseri | |
3.5.1.24 | Tween 80 | - |
Lactobacillus gasseri |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.24 | gene bsh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Eschericchia coli strain DH5alpha | Lactobacillus gasseri |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.24 | ampicillin | almost complete inhibition | Lactobacillus gasseri | |
3.5.1.24 | ascorbic acid | moderate inhibition | Lactobacillus gasseri | |
3.5.1.24 | CaCl2 | slight inhibition | Lactobacillus gasseri | |
3.5.1.24 | CuCl2 | strong inhibition | Lactobacillus gasseri | |
3.5.1.24 | CuSO4 | strong inhibition | Lactobacillus gasseri | |
3.5.1.24 | doxycycline hydrochloride | almost complete inhibition | Lactobacillus gasseri | |
3.5.1.24 | erythromycin | moderate inhibition | Lactobacillus gasseri | |
3.5.1.24 | ethanol | slight inhibition | Lactobacillus gasseri | |
3.5.1.24 | KIO3 | strong inhibition | Lactobacillus gasseri | |
3.5.1.24 | Lincomycin | slight inhibition | Lactobacillus gasseri | |
3.5.1.24 | methanol | slight inhibition | Lactobacillus gasseri | |
3.5.1.24 | MgCl2 | - |
Lactobacillus gasseri | |
3.5.1.24 | MgSO4 | - |
Lactobacillus gasseri | |
3.5.1.24 | MnCl2 | - |
Lactobacillus gasseri | |
3.5.1.24 | MnSO4 | - |
Lactobacillus gasseri | |
3.5.1.24 | additional information | chloroform, 2-mercaptoethanol and EDTA show poor effects on the enzyme activity. Proteinase K almost completely abolishes the enzyme activity, strong inhibition by pepsin. The enzyme is not affected by alpha-amylase and catalase, and only slightly by lysozyme | Lactobacillus gasseri | |
3.5.1.24 | NaHIO3 | strong inhibition | Lactobacillus gasseri | |
3.5.1.24 | neomycin | strong inhibition | Lactobacillus gasseri | |
3.5.1.24 | oxytetracycline | almost complete inhibition | Lactobacillus gasseri | |
3.5.1.24 | Penicillin V | almost complete inhibition | Lactobacillus gasseri | |
3.5.1.24 | riboflavin | almost complete inhibition | Lactobacillus gasseri | |
3.5.1.24 | SDS | almost complete inhibition | Lactobacillus gasseri | |
3.5.1.24 | ZnCl2 | - |
Lactobacillus gasseri | |
3.5.1.24 | ZnSO4 | - |
Lactobacillus gasseri |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.24 | glycocholate + H2O | Lactobacillus gasseri | - |
cholate + glycine | - |
? | |
3.5.1.24 | glycocholate + H2O | Lactobacillus gasseri FR4 | - |
cholate + glycine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.24 | Lactobacillus gasseri | B9V405 | - |
- |
3.5.1.24 | Lactobacillus gasseri FR4 | B9V405 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.24 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Lactobacillus gasseri |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.24 | glycocholate + H2O | - |
Lactobacillus gasseri | cholate + glycine | - |
? | |
3.5.1.24 | glycocholate + H2O | - |
Lactobacillus gasseri FR4 | cholate + glycine | - |
? | |
3.5.1.24 | additional information | purified recombinant LgBSH exhibits substrate specificity toward glyco-conjugated bile salts (GCA and GDCA) compared to tauro-conjugated bile salts. Substrate specificity of LgBSH, overview | Lactobacillus gasseri | ? | - |
? | |
3.5.1.24 | additional information | purified recombinant LgBSH exhibits substrate specificity toward glyco-conjugated bile salts (GCA and GDCA) compared to tauro-conjugated bile salts. Substrate specificity of LgBSH, overview | Lactobacillus gasseri FR4 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.24 | ? | x * 37000, recombinant enzyme, SDS-PAGE | Lactobacillus gasseri |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.24 | bile salt hydrolase | - |
Lactobacillus gasseri |
3.5.1.24 | BSH | - |
Lactobacillus gasseri |
3.5.1.24 | LgBSH | - |
Lactobacillus gasseri |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.24 | 52 | - |
recombinant enzyme | Lactobacillus gasseri |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.24 | 20 | 70 | activity range, profile overview | Lactobacillus gasseri |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.24 | 5.5 | - |
recombinant enzyme | Lactobacillus gasseri |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.24 | 3 | 8 | activity range, profile overview | Lactobacillus gasseri |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.5.1.24 | additional information | enzyme structure homology model of LgBSH using the structure of Enterococcus faecalis BSH (PDB ID 4WL3) as a template. Residues involved in catalysis are identified based on the superimposed structure, including Cys1, Arg16, Asp19, Asn79, Asn171, and Arg224. Molecular docking analysis of ligands | Lactobacillus gasseri |