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Literature summary extracted from

  • Gimenez, M.; Cerletti, M.; De Castro, R.
    Archaeal membrane-associated proteases Insights on Haloferax volcanii and other haloarchaea (2015), Front. Microbiol., 6, 39 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.53 crystal structure determination at 2.0 A resolution Thermococcus onnurineus
3.4.21.53 crystal structure determination of LonB proteolytic domain Archaeoglobus fulgidus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.53 cell membrane has two transmembrane domains Natrialba magadii
-
-
3.4.21.53 cell membrane has two transmembrane domains Haloferax volcanii
-
-
3.4.21.53 membrane has one transmembrane domains Archaeoglobus fulgidus 16020
-
3.4.21.53 membrane has two transmembrane domains Thermococcus kodakarensis 16020
-
3.4.21.53 membrane has two transmembrane domains Thermoplasma acidophilum 16020
-
3.4.21.53 membrane has two transmembrane domains Thermococcus onnurineus 16020
-
3.4.23.52 additional information the enzyme contains a signal peptide Haloferax volcanii
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.53 Mg2+ required Thermococcus kodakarensis
3.4.21.53 Mg2+ required Archaeoglobus fulgidus
3.4.21.53 Mg2+ required Thermoplasma acidophilum
3.4.21.53 Mg2+ required Natrialba magadii
3.4.21.53 Mg2+ required Thermococcus onnurineus
3.4.21.53 Mg2+ required Haloferax volcanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.23.43 preflagellin + H2O Haloferax volcanii
-
 flagellin + preflagellin leader peptide
-
 ?
3.4.23.43 preflagellin + H2O Saccharolobus solfataricus
-
 flagellin + preflagellin leader peptide
-
 ?
3.4.23.43 prepilin type IV + H2O Haloferax volcanii
-
 pilin type IV + prepilin type IV leader peptide
-
 ?
3.4.23.43 prepilin type IV + H2O Saccharolobus solfataricus
-
 pilin type IV + prepilin type IV leader peptide
-
 ?
3.4.23.52 preflagellin + H2O Haloferax volcanii
-
 flagellin + ?
-
 ?
3.4.23.52 preflagellin + H2O Haloferax volcanii ATCC 29605
-
 flagellin + ?
-
 ?
3.4.23.52 prepilin + H2O Haloferax volcanii
-
 pilin + ?
-
 ?
3.4.23.52 prepilin + H2O Haloferax volcanii ATCC 29605
-
 pilin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.53 Archaeoglobus fulgidus O29883
-
-
3.4.21.53 Archaeoglobus fulgidus ATCC 49558 O29883
-
-
3.4.21.53 Archaeoglobus fulgidus JCM 9628 O29883
-
-
3.4.21.53 Archaeoglobus fulgidus NBRC 100126 O29883
-
-
3.4.21.53 Archaeoglobus fulgidus VC-16 O29883
-
-
3.4.21.53 Haloferax volcanii D4GTT9
-
-
3.4.21.53 Haloferax volcanii ATCC 29605 D4GTT9
-
-
3.4.21.53 Haloferax volcanii DS2 D4GTT9
-
-
3.4.21.53 Haloferax volcanii DSM 3757 D4GTT9
-
-
3.4.21.53 Haloferax volcanii JCM 8879 D4GTT9
-
-
3.4.21.53 Haloferax volcanii NBRC 14742 D4GTT9
-
-
3.4.21.53 Haloferax volcanii NCIMB 2012 D4GTT9
-
-
3.4.21.53 Haloferax volcanii VKM B-1768 D4GTT9
-
-
3.4.21.53 Natrialba magadii C5MK48
-
-
3.4.21.53 Natrialba magadii ATCC 43099 C5MK48
-
-
3.4.21.53 Natrialba magadii CIP 104546 C5MK48
-
-
3.4.21.53 Natrialba magadii DSM 3394 C5MK48
-
-
3.4.21.53 Natrialba magadii JCM 8861 C5MK48
-
-
3.4.21.53 Natrialba magadii MS3 C5MK48
-
-
3.4.21.53 Natrialba magadii NBRC 102185 C5MK48
-
-
3.4.21.53 Natrialba magadii NCIMB 2190 C5MK48
-
-
3.4.21.53 Thermococcus kodakarensis Q8NKS6
-
-
3.4.21.53 Thermococcus onnurineus B6YU74
-
-
3.4.21.53 Thermoplasma acidophilum Q9HJ89
-
-
3.4.21.53 Thermoplasma acidophilum AMRC-C165 Q9HJ89
-
-
3.4.21.53 Thermoplasma acidophilum ATCC 25905 Q9HJ89
-
-
3.4.21.53 Thermoplasma acidophilum JCM 9062 Q9HJ89
-
-
3.4.21.53 Thermoplasma acidophilum NBRC 15155 Q9HJ89
-
-
3.4.23.43 Haloferax volcanii
-
-
-
3.4.23.43 Saccharolobus solfataricus
-
-
-
3.4.23.52 Haloferax volcanii D4GY85
-
-
3.4.23.52 Haloferax volcanii ATCC 29605 D4GY85
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.53 additional information LonB protease showed ATPase and protease activities. The enzyme has DNA-binding capacity in vitro Natrialba magadii ?
-
 ?
3.4.21.53 additional information LonB protease shows ATPase and protease activities Archaeoglobus fulgidus ?
-
 ?
3.4.21.53 additional information LonB protease shows ATPase and protease activities Thermococcus onnurineus ?
-
 ?
3.4.21.53 additional information LonB protease shows ATPase and protease activities Haloferax volcanii ?
-
 ?
3.4.21.53 additional information LonB protease shows ATPase and protease activities. Membrane-bound ATP-dependent Lon protease from Thermococcus kodakaraensis shows ATP-independent activity on unfolded substrates and ATP-dependent activity on folded proteins Thermococcus kodakarensis ?
-
 ?
3.4.21.53 additional information recombinant LonB protease shows ATPase and protease activities Thermoplasma acidophilum ?
-
 ?
3.4.21.53 additional information LonB protease showed ATPase and protease activities. The enzyme has DNA-binding capacity in vitro Natrialba magadii ATCC 43099 ?
-
 ?
3.4.21.53 additional information LonB protease shows ATPase and protease activities Haloferax volcanii NCIMB 2012 ?
-
 ?
3.4.21.53 additional information LonB protease showed ATPase and protease activities. The enzyme has DNA-binding capacity in vitro Natrialba magadii NBRC 102185 ?
-
 ?
3.4.21.53 additional information LonB protease shows ATPase and protease activities Archaeoglobus fulgidus NBRC 100126 ?
-
 ?
3.4.21.53 additional information LonB protease showed ATPase and protease activities. The enzyme has DNA-binding capacity in vitro Natrialba magadii DSM 3394 ?
-
 ?
3.4.21.53 additional information LonB protease shows ATPase and protease activities Archaeoglobus fulgidus ATCC 49558 ?
-
 ?
3.4.21.53 additional information LonB protease shows ATPase and protease activities Haloferax volcanii JCM 8879 ?
-
 ?
3.4.21.53 additional information LonB protease showed ATPase and protease activities. The enzyme has DNA-binding capacity in vitro Natrialba magadii NCIMB 2190 ?
-
 ?
3.4.21.53 additional information recombinant LonB protease shows ATPase and protease activities Thermoplasma acidophilum JCM 9062 ?
-
 ?
3.4.21.53 additional information recombinant LonB protease shows ATPase and protease activities Thermoplasma acidophilum AMRC-C165 ?
-
 ?
3.4.21.53 additional information LonB protease showed ATPase and protease activities. The enzyme has DNA-binding capacity in vitro Natrialba magadii JCM 8861 ?
-
 ?
3.4.21.53 additional information LonB protease showed ATPase and protease activities. The enzyme has DNA-binding capacity in vitro Natrialba magadii CIP 104546 ?
-
 ?
3.4.21.53 additional information LonB protease shows ATPase and protease activities Archaeoglobus fulgidus VC-16 ?
-
 ?
3.4.21.53 additional information LonB protease shows ATPase and protease activities Haloferax volcanii DS2 ?
-
 ?
3.4.21.53 additional information LonB protease showed ATPase and protease activities. The enzyme has DNA-binding capacity in vitro Natrialba magadii MS3 ?
-
 ?
3.4.21.53 additional information recombinant LonB protease shows ATPase and protease activities Thermoplasma acidophilum ATCC 25905 ?
-
 ?
3.4.21.53 additional information LonB protease shows ATPase and protease activities Haloferax volcanii DSM 3757 ?
-
 ?
3.4.21.53 additional information LonB protease shows ATPase and protease activities Archaeoglobus fulgidus JCM 9628 ?
-
 ?
3.4.21.53 additional information recombinant LonB protease shows ATPase and protease activities Thermoplasma acidophilum NBRC 15155 ?
-
 ?
3.4.21.53 additional information LonB protease shows ATPase and protease activities Haloferax volcanii ATCC 29605 ?
-
 ?
3.4.21.53 additional information LonB protease shows ATPase and protease activities Haloferax volcanii NBRC 14742 ?
-
 ?
3.4.21.53 additional information LonB protease shows ATPase and protease activities Haloferax volcanii VKM B-1768 ?
-
 ?
3.4.23.43 preflagellin + H2O
-
 Haloferax volcanii flagellin + preflagellin leader peptide
-
 ?
3.4.23.43 preflagellin + H2O
-
 Saccharolobus solfataricus flagellin + preflagellin leader peptide
-
 ?
3.4.23.43 prepilin type IV + H2O
-
 Haloferax volcanii pilin type IV + prepilin type IV leader peptide
-
 ?
3.4.23.43 prepilin type IV + H2O
-
 Saccharolobus solfataricus pilin type IV + prepilin type IV leader peptide
-
 ?
3.4.23.52 preflagellin + H2O
-
 Haloferax volcanii flagellin + ?
-
 ?
3.4.23.52 preflagellin + H2O
-
 Haloferax volcanii ATCC 29605 flagellin + ?
-
 ?
3.4.23.52 prepilin + H2O
-
 Haloferax volcanii pilin + ?
-
 ?
3.4.23.52 prepilin + H2O
-
 Haloferax volcanii ATCC 29605 pilin + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.53 AF0364
-
 Archaeoglobus fulgidus
3.4.21.53 ATP-dependent lon protease
-
 Thermococcus kodakarensis
3.4.21.53 ATP-dependent lon protease
-
 Archaeoglobus fulgidus
3.4.21.53 ATP-dependent lon protease
-
 Thermoplasma acidophilum
3.4.21.53 ATP-dependent lon protease
-
 Natrialba magadii
3.4.21.53 ATP-dependent lon protease
-
 Thermococcus onnurineus
3.4.21.53 ATP-dependent lon protease
-
 Haloferax volcanii
3.4.21.53 Nmag_2822
-
 Natrialba magadii
3.4.21.53 NmLon
-
 Natrialba magadii
3.4.21.53 Ta1081
-
 Thermoplasma acidophilum
3.4.21.53 TK1264
-
 Thermococcus kodakarensis
3.4.21.53 TON_0529
-
 Thermococcus onnurineus
3.4.23.43 PibD
-
 Haloferax volcanii
3.4.23.43 PibD
-
 Saccharolobus solfataricus
3.4.23.43 type IV prepilin peptidase
-
 Haloferax volcanii
3.4.23.43 type IV prepilin peptidase
-
 Saccharolobus solfataricus
3.4.23.52 PibD
-
 Haloferax volcanii
3.4.23.52 type IV prepilin peptidase
-
 Haloferax volcanii

Cofactor

EC Number Cofactor Comment Organism Structure
3.4.21.53 ATP dependent on Thermococcus kodakarensis
3.4.21.53 ATP dependent on Archaeoglobus fulgidus
3.4.21.53 ATP dependent on Thermoplasma acidophilum
3.4.21.53 ATP dependent on Natrialba magadii
3.4.21.53 ATP dependent on Thermococcus onnurineus
3.4.21.53 ATP dependent on Haloferax volcanii

General Information

EC Number General Information Comment Organism
3.4.21.53 malfunction suboptimal LonB expression affects the content of membrane carotenoids and other lipids. Haloferax volcanii mutant cells deficient in Lon content are more sensitive to puromycin compared to wild-type cells. Deregulation of the cellular concentration of bacterioruberins and other lipids affects membrane stability contributing to the lethal phenotype of the lon knockout mutant Haloferax volcanii
3.4.21.53 additional information the enzyme has a Ser-Lys catalytic dyad Thermoplasma acidophilum
3.4.21.53 physiological function membrane-bound ATP-dependent Lon protease is essential for cell viability and quality control of proteins, it affects membrane carotenoid content in Haloferax volanii. Enzyme LonB controls carotenoid biosynthesis in Haloferax volcanii probably by degrading enzyme/s involved in this pathway. LonB is implicated in bacterioruberin biosynthesis and protein quality control Haloferax volcanii
3.4.21.53 physiological function the enzyme can complement a LonB mutant in Haloferax volcanii suggesting functional conservation of LonB Natrialba magadii
3.4.23.43 metabolism the enzyme is needed for maturation of preflagellins and other type IV pilin-like proteins Haloferax volcanii
3.4.23.43 metabolism the enzyme is needed for maturation of preflagellins and other type IV pilin-like proteins Saccharolobus solfataricus
3.4.23.52 physiological function type IV prepilin peptidase PibD processes flagellin/pilin precursors, that are essential for the biogenesis and function of the archaellum and other cell surface structures in Haloferax volcanii Haloferax volcanii