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Literature summary extracted from

  • Zarzecka, U.; Modrak-Wojcik, A.; Figaj, D.; Apanowicz, M.; Lesner, A.; Bzowska, A.; Lipinska, B.; Zawilak-Pawlik, A.; Backert, S.; Skorko-Glonek, J.
    Properties of the HtrA protease from bacterium Helicobacter pylori whose activity is indispensable for growth under stress conditions (2019), Front. Microbiol., 10, 961 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.107 expression in Escherichia coli Helicobacter pylori

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.107 S221A loss of catalytic activity Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.107 Helicobacter pylori Q9ZM18
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.107 recombinant enzyme. Comparison of the protein from Helicobacter pylori strains 26695, J99 and N6 Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.107 beta-casein + H2O
-
 Helicobacter pylori ?
-
 ?
3.4.21.107 additional information enzyme cuts preferentially after valine at P1, with serine, leucine and isoleucine in decreasing order of preference. No substrate: native lysozyme Helicobacter pylori ?
-
 ?
3.4.21.107 reduced lysozyme + H2O
-
 Helicobacter pylori ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.107 70 75
-
 Helicobacter pylori

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.107 85
-
 melting temperature above 85°C, melting temepratures at pH 5.5, 6.5, or 8.0 are similar Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.107 6 6.5
-
 Helicobacter pylori

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.107 5.5 7
-
 Helicobacter pylori

General Information

EC Number General Information Comment Organism
3.4.21.107 physiological function a HtrA knockout strain shows increased sensitivity to stress such as elevated temperature, pH and osmotic shock, as well as treatment with puromycin Helicobacter pylori