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Literature summary extracted from

  • Sharma, N.; Rao, S.P.; Kalivendi, S.V.
    The deglycase activity of DJ-1 mitigates alpha-synuclein glycation and aggregation in dopaminergic cells Role of oxidative stress mediated downregulation of DJ-1 in Parkinsons disease (2019), Free Radic. Biol. Med., 135, 28-37 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.124 DJ-1 gene is subcloned from pLenti6-DJ-1-V5-WT plasmid into the pRetroX-Tight-Puro Advanced vector. The confirmed clone is transfected into HEK-293 cells Homo sapiens
4.2.1.130 DJ-1 gene is subcloned from pLenti6-DJ-1-V5-WT plasmid into the pRetroX-Tight-Puro Advanced vector. The confirmed clone is transfected into HEK-293 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.130 L166P the Km-value for methylglyoxal of the mutant enzyme is about 2fold higher compared to the wild-type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.130 900
-
 methylglyoxal 30°C, pH not specified in the publication, wild-type enzyme Homo sapiens
4.2.1.130 1900
-
 methylglyoxal 30°C, pH not specified in the publication, mutant enzyme L166P Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.130 methylglyoxal + H2O Homo sapiens
-
 (R)-lactate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.124 Homo sapiens Q99497 cf. EC 4.2.1.130
-
4.2.1.130 Homo sapiens Q99497 cf. EC 3.5.1.124
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.124
-
 Homo sapiens
4.2.1.130
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.124 N6-(1-hydroxy-2-oxopropyl)-[alpha-synuclein]-L-lysine + H2O
-
 Homo sapiens [alpha-synuclein]-L-lysine + lactate
-
 ?
4.2.1.130 methylglyoxal + H2O
-
 Homo sapiens (R)-lactate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.124 DJ-1
-
 Homo sapiens
4.2.1.130 DJ-1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.124 30
-
 assay at Homo sapiens
4.2.1.130 30
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
3.5.1.124 malfunction reduced DJ-1 activity due to mutations or oxidative stress may lead to the accumulation of glycated alpha-synuclein and its aggregates Homo sapiens
3.5.1.124 physiological function the deglycase activity of DJ-1 mitigates alpha-synuclein glycation and aggregation in dopaminergic cells Homo sapiens