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Literature summary extracted from
- Purification and partial characterization of an X-prolyl-dipeptidyl aminopeptidase from lactobacillus gasseri me-284 (2015), Food Sci. Technol. Res., 21, 445-451 .
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.13.9 | EDTA | slight activation at 1.0 mM | Lactobacillus gasseri |  |
| 3.4.13.9 | additional information | no significant effect by 2-mercaptoethanol at 1 mM | Lactobacillus gasseri | |
| 3.4.14.11 | EDTA | slight activation at 1.0 mM | Lactobacillus gasseri | |
| 3.4.14.11 | additional information | no significant effect by 2-mercaptoethanol at 1 mM | Lactobacillus gasseri |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.13.9 | Co2+ | slight inhibition at 0.1-1.0 mM | Lactobacillus gasseri | |
| 3.4.13.9 | Cu2+ | 85% inhibition at 1 mM | Lactobacillus gasseri | |
| 3.4.13.9 | diisopropylfluorophosphate | 87% inhibition at 1 mM | Lactobacillus gasseri | |
| 3.4.13.9 | Diprotin A | - |
Lactobacillus gasseri | |
| 3.4.13.9 | Hg2+ | 98% inhibition at 1 mM | Lactobacillus gasseri | |
| 3.4.13.9 | Mg2+ | slight inhibition at 0.1-1.0 mM | Lactobacillus gasseri | |
| 3.4.13.9 | additional information | no significant effect by 2-mercaptoethanol at 1 mM | Lactobacillus gasseri | |
| 3.4.13.9 | Phenylmethylsulfonylfluoride | 88.5% inhibition at 1 mM | Lactobacillus gasseri | |
| 3.4.13.9 | Zn2+ | 56% inhibition at 1 mM | Lactobacillus gasseri | |
| 3.4.14.11 | Co2+ | slight inhibition at 0.1-1.0 mM | Lactobacillus gasseri | |
| 3.4.14.11 | Cu2+ | 85% inhibition at 1 mM | Lactobacillus gasseri | |
| 3.4.14.11 | diisopropylfluorophosphate | 87% inhibition at 1 mM | Lactobacillus gasseri | |
| 3.4.14.11 | Diprotin A | - |
Lactobacillus gasseri | |
| 3.4.14.11 | Hg2+ | 98% inhibition at 1 mM | Lactobacillus gasseri | |
| 3.4.14.11 | Mg2+ | slight inhibition at 0.1-1.0 mM | Lactobacillus gasseri | |
| 3.4.14.11 | additional information | no significant effect by 2-mercaptoethanol at 1 mM | Lactobacillus gasseri | |
| 3.4.14.11 | Phenylmethylsulfonylfluoride | 88.5% inhibition at 1 mM | Lactobacillus gasseri | |
| 3.4.14.11 | Zn2+ | 56% inhibition at 1 mM | Lactobacillus gasseri | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.13.9 | additional information | no significant effect by Ca2+ at 1 mM | Lactobacillus gasseri | |
| 3.4.14.11 | additional information | no significant effect by Ca2+ at 1 mM | Lactobacillus gasseri |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.13.9 | 173000 | - |
gel filtration | Lactobacillus gasseri |
| 3.4.14.11 | 173000 | - |
gel filtration | Lactobacillus gasseri |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.13.9 | Lactobacillus gasseri | - |
- |
- |
| 3.4.13.9 | Lactobacillus gasseri ATCC 33323 | - |
- |
- |
| 3.4.13.9 | Lactobacillus gasseri me-284 | - |
- |
- |
| 3.4.14.11 | Lactobacillus gasseri | - |
- |
- |
| 3.4.14.11 | Lactobacillus gasseri ATCC 33323 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.13.9 | native enzyme 265fold in a seven step process, i.e. two steps of anion exchange chromatography, adsorption and hydrophobic interaction chromatography, two steps of gel filtration, and heparin affinity chromatography | Lactobacillus gasseri |
| 3.4.14.11 | native enzyme 265fold in a seven step process, i.e, two steps of anion exchange chromatography, adsorption and hydrophobic interaction chromatography, two steps of gel filtration, and heparin affinity chromatography | Lactobacillus gasseri |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.13.9 | Gly-Pro-4-nitroanilide + H2O | - |
Lactobacillus gasseri | Gly + Pro-4-nitroanilide | - |
? | |
| 3.4.13.9 | Gly-Pro-4-nitroanilide + H2O | - |
Lactobacillus gasseri me-284 | Gly + Pro-4-nitroanilide | - |
? | |
| 3.4.13.9 | Gly-Pro-4-nitroanilide + H2O | - |
Lactobacillus gasseri ATCC 33323 | Gly + Pro-4-nitroanilide | - |
? | |
| 3.4.13.9 | additional information | the enzyme is likely a serine proteinase | Lactobacillus gasseri | ? | - |
? | |
| 3.4.13.9 | additional information | the enzyme is likely a serine proteinase | Lactobacillus gasseri me-284 | ? | - |
? | |
| 3.4.13.9 | additional information | the enzyme is likely a serine proteinase | Lactobacillus gasseri ATCC 33323 | ? | - |
? | |
| 3.4.13.9 | Phe-Pro-Gly-Pro-Ile + H2O | - |
Lactobacillus gasseri | Phe + Pro-Gly-Pro-Ile | - |
? | |
| 3.4.13.9 | Phe-Pro-Gly-Pro-Ile + H2O | - |
Lactobacillus gasseri me-284 | Phe + Pro-Gly-Pro-Ile | - |
? | |
| 3.4.13.9 | Phe-Pro-Gly-Pro-Ile + H2O | - |
Lactobacillus gasseri ATCC 33323 | Phe + Pro-Gly-Pro-Ile | - |
? | |
| 3.4.13.9 | Tyr-Pro-Phe-Pro-Gly-Pro-Ile + H2O | i.e. BCM-7, beta-casomorphin 7 | Lactobacillus gasseri | Tyr + Pro-Phe-Pro-Gly-Pro-Ile | - |
? | |
| 3.4.13.9 | Tyr-Pro-Phe-Pro-Gly-Pro-Ile + H2O | i.e. BCM-7, beta-casomorphin 7 | Lactobacillus gasseri me-284 | Tyr + Pro-Phe-Pro-Gly-Pro-Ile | - |
? | |
| 3.4.13.9 | Tyr-Pro-Phe-Pro-Gly-Pro-Ile + H2O | i.e. BCM-7, beta-casomorphin 7 | Lactobacillus gasseri ATCC 33323 | Tyr + Pro-Phe-Pro-Gly-Pro-Ile | - |
? | |
| 3.4.14.11 | additional information | the enzyme is likely a serine proteinase | Lactobacillus gasseri | ? | - |
? | |
| 3.4.14.11 | additional information | the enzyme is likely a serine proteinase | Lactobacillus gasseri me-284 | ? | - |
? | |
| 3.4.14.11 | additional information | the enzyme is likely a serine proteinase | Lactobacillus gasseri ATCC 33323 | ? | - |
? | |
| 3.4.14.11 | Pro-Phe-Pro-Gly-Pro-Ile + H2O | - |
Lactobacillus gasseri | Pro-Phe + Pro-Gly-Pro-Ile | - |
? | |
| 3.4.14.11 | Tyr-Pro-Phe-Pro-Gly-Pro-Ile + H2O | i.e. BCM-7, beta-casomorphin 7 | Lactobacillus gasseri | Tyr-Pro + Phe-Pro-Gly-Pro-Ile | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.13.9 | dimer | 2 * 82000, SDS-PAGE | Lactobacillus gasseri |
| 3.4.14.11 | dimer | 2 * 82000, SDS-PAGE | Lactobacillus gasseri |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.13.9 | LGAS_0712 | - |
Lactobacillus gasseri |
| 3.4.13.9 | More | see also EC 3.4.14.11 | Lactobacillus gasseri |
| 3.4.13.9 | X-prolyl-dipeptidyl aminopeptidase | - |
Lactobacillus gasseri |
| 3.4.14.11 | LGAS_0712 | - |
Lactobacillus gasseri |
| 3.4.14.11 | More | see also EC 3.4.13.9 | Lactobacillus gasseri |
| 3.4.14.11 | X-prolyl-dipeptidyl aminopeptidase | - |
Lactobacillus gasseri |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.13.9 | 55 | - |
assay at | Lactobacillus gasseri |
| 3.4.14.11 | 55 | - |
assay at | Lactobacillus gasseri |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.13.9 | 7 | - |
assay at | Lactobacillus gasseri |
| 3.4.14.11 | 7 | - |
assay at | Lactobacillus gasseri |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 3.4.13.9 | 0.0244 | - |
pH 7.0, 55°C, recombinant enzyme | Lactobacillus gasseri | Diprotin A | |
| 3.4.14.11 | 0.0244 | - |
pH 7.0, 55°C, recombinant enzyme | Lactobacillus gasseri | Diprotin A | |
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