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Literature summary extracted from

  • Ramli, A.N.M.; Manas, N.H.A.; Hamid, A.A.A.; Hamid, H.A.; Illias, R.M.
    Comparative structural analysis of fruit and stem bromelain from Ananas comosus (2018), Food Chem., 266, 183-191 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.22.32 medicine bromelain has gained wide acceptance and compliance as a phytotherapeutical drug. Cysteine proteases in pineapple (Ananas comosus) plants are phytotherapeutical agents that demonstrate anti-edematous, anti-inflammatory, anti-thrombotic and fibrinolytic activities Ananas comosus
3.4.22.33 medicine bromelain has gained wide acceptance and compliance as a phytotherapeutical drug. Cysteine proteases in pineapple (Ananas comosus) plants are phytotherapeutical agents that demonstrate antiedematous, anti-inflammatory, anti-thrombotic and fibrinolytic activities Ananas comosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.32 Ananas comosus P14518
-
-
3.4.22.33 Ananas comosus O23791
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.32 stem
-
 Ananas comosus
-
3.4.22.33 fruit
-
 Ananas comosus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.32 benzyl-Phe-Val-Arg-4-nitroanilide + H2O
-
 Ananas comosus benzyl-Phe-Val-Arg + 4-nitroaniline
-
 ?
3.4.22.32 benzyloxycarbonyl-Arg-Arg-4-nitroanilide + H2O preferred substrate of stem bromelain Ananas comosus benzyloxycarbonyl-Arg-Arg + 4-nitroaniline
-
 ?
3.4.22.33 benzyl-Phe-Val-Arg-4-nitroanilide + H2O preferred substrate of fruit bromelain Ananas comosus benzyl-Phe-Val-Arg + 4-nitroaniline
-
 ?
3.4.22.33 benzyloxycarbonyl-Arg-Arg-4-nitroanilide + H2O
-
 Ananas comosus benzyloxycarbonyl-Arg-Arg + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.32 ? 23400-35730, stem bromelain, SDS-PAGE Ananas comosus
3.4.22.32 More comparative structural analysis of fruit and stem bromelain from Ananas comosus Ananas comosus
3.4.22.33 ? x * 25000-31000, fruit bromelain, SDS-PAGE Ananas comosus
3.4.22.33 More comparative structural analysis of fruit and stem bromelain from Ananas comosus Ananas comosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.22.32 Ananas comosus isoelectric focusing
-
 9.55
3.4.22.33 Ananas comosus isoelectric focusing
-
 4.6

General Information

EC Number General Information Comment Organism
3.4.22.32 additional information comparative structural analysis of fruit and stem bromelain from Ananas comosus, structure homology modelling, model domain organisation, overview. The proteolytic fraction of pineapple stem is termed stem bromelain, while the one presents in the fruit is known as fruit bromelain (EC 3.4.22.33). NCBI conserved domain analysis reveals two domains of fruit and stem bromelain: cathepsin propeptide inhibitor (I29) and peptidase C1 Ananas comosus
3.4.22.33 additional information comparative structural analysis of fruit and stem bromelain from Ananas comosus, structure homology modelling, model domain organisation, overview. The proteolytic fraction of pineapple stem is termed stem bromelain, while the one presents in the fruit is known as fruit bromelain (EC 3.4.22.33). NCBI conserved domain analysis reveals two domains of fruit and stem bromelain: cathepsin propeptide inhibitor (I29) and peptidase C1 Ananas comosus