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Literature summary extracted from

  • Mora-Montes, H.; Lopez-Romero, E.; Zinker, S.; Ponce-Noyola, P.; Flores-Carreon, A.
    Purification of soluble alpha1,2-mannosidase from Candida albicans CAI-4 (2006), FEMS Microbiol. Lett., 256, 50-56 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.113 1-deoxymannojirimycin
-
 Candida albicans
3.2.1.113 swainsonine
-
 Candida albicans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.113 0.09
-
 4-methylumbelliferyl alpha-D-mannopyranoside at pH 6.0 in 50 mM Tris-MES and at 37°C Candida albicans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.113 soluble
-
 Candida albicans
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.113 Candida albicans
-
-
-
3.2.1.113 Candida albicans CAI-4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.113 Sepharose CL6B column chromatography, DEAE Bio-Gel A column chromatography, Concanavalin A-Sepharose column chromatography, and Sephadex G-25 gel filtration Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.113 4-methylumbelliferyl alpha-D-mannopyranoside + H2O
-
 Candida albicans 4-methylumbelliferone + alpha-D-mannopyranose
-
 ?
3.2.1.113 4-methylumbelliferyl alpha-D-mannopyranoside + H2O
-
 Candida albicans CAI-4 4-methylumbelliferone + alpha-D-mannopyranose
-
 ?
3.2.1.113 Man9GlcNAc2-Asn + 4 H2O
-
 Candida albicans Man5GlcNAc2-Asn + 4 beta-D-mannose
-
 ?
3.2.1.113 Man9GlcNAc2-Asn + 4 H2O
-
 Candida albicans CAI-4 Man5GlcNAc2-Asn + 4 beta-D-mannose
-
 ?
3.2.1.113 Man9GlcNAc2-Asn + H2O
-
 Candida albicans Man8GlcNAc2-Asn (isomer 8A1,2,3B1,3) + D-mannose
-
 ?
3.2.1.113 Man9GlcNAc2-Asn + H2O
-
 Candida albicans CAI-4 Man8GlcNAc2-Asn (isomer 8A1,2,3B1,3) + D-mannose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.113 ? x * 52000, SDS-PAGE Candida albicans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.113 alpha1,2-mannosidase
-
 Candida albicans

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.113 0.23
-
 at pH 6.0 in 50 mM Tris-MES and at 37°C Candida albicans 1-deoxymannojirimycin
3.2.1.113 0.47
-
 at pH 6.0 in 50 mM Tris-MES and at 37°C Candida albicans swainsonine