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Literature summary extracted from

  • Lehmacher, A.; Vogt, A.B.; Hensel, R.
    Biosynthesis of cyclic 2,3-diphosphoglycerate. Isolation and characterization of 2-phosphoglycerate kinase and cyclic 2,3-diphosphoglycerate synthetase from Methanothermus fervidus (1990), FEBS Lett., 272, 94-98 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.16 EDTA 5 mM, almost complete inhibition Methanothermus fervidus
6.5.1.9 EDTA the enzyme is almost completely inhibited by 5 mM EDTA Methanothermus fervidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.16 2.7
-
 ATP pH 7.5, 75°C Methanothermus fervidus
6.5.1.9 1.3
-
 ATP at pH 7.0 and 75°C Methanothermus fervidus
6.5.1.9 5.5
-
 2,3-diphospho-D-glycerate at pH 7.0 and 75°C Methanothermus fervidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.16 K+ 0.3 M, 1.4fold stimulation Methanothermus fervidus
2.7.2.16 Mg2+ required, 8% residual activity in absence of Mg2+ Methanothermus fervidus
6.5.1.9 K+ the maximally (2.4fold) activating KC1 concentration is 0.5 M Methanothermus fervidus
6.5.1.9 Mg2+ required for activity Methanothermus fervidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.16 1400000
-
 gel filtration Methanothermus fervidus
6.5.1.9 145000
-
 gel filtration Methanothermus fervidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.9 ATP + 2,3-diphospho-D-glycerate Methanothermus fervidus
-
 ADP + phosphate + cyclic 2,3-bisphosphoglycerate
-
 ?
6.5.1.9 ATP + 2,3-diphospho-D-glycerate Methanothermus fervidus DSM 2088
-
 ADP + phosphate + cyclic 2,3-bisphosphoglycerate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.16 Methanothermus fervidus Q49156
-
-
2.7.2.16 Methanothermus fervidus ATCC 43054 Q49156
-
-
6.5.1.9 Methanothermus fervidus
-
-
-
6.5.1.9 Methanothermus fervidus DSM 2088
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.16
-
 Methanothermus fervidus
6.5.1.9 Q-Sepharose column chromatography, hydroxyapatite column chromatography, ATP-agarose column chromatography, and 2,3-diphospho-D-glycerate-agarose column chromatography Methanothermus fervidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.16 200
-
 pH 7.5, 75°C Methanothermus fervidus
6.5.1.9 0.02
-
 crude extract, at pH 7.0 and 75°C Methanothermus fervidus
6.5.1.9 20.6
-
 after 1030fold purification, at pH 7.0 and 75°C Methanothermus fervidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.16 ATP + 2-phospho-D-glycerate
-
 Methanothermus fervidus ADP + 2,3-diphospho-D-glycerate
-
 ?
2.7.2.16 ATP + 2-phospho-D-glycerate
-
 Methanothermus fervidus ATCC 43054 ADP + 2,3-diphospho-D-glycerate
-
 ?
6.5.1.9 ATP + 2,3-diphospho-D-glycerate
-
 Methanothermus fervidus ADP + phosphate + cyclic 2,3-bisphosphoglycerate
-
 ?
6.5.1.9 ATP + 2,3-diphospho-D-glycerate
-
 Methanothermus fervidus DSM 2088 ADP + phosphate + cyclic 2,3-bisphosphoglycerate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.16 multimer x * 32500, SDS-PAGE Methanothermus fervidus
6.5.1.9 homotetramer 4 * 38000, SDS-PAGE Methanothermus fervidus

Synonyms

EC Number Synonyms Comment Organism
2.7.2.16 2PGK
-
 Methanothermus fervidus
6.5.1.9 cDPGs
-
 Methanothermus fervidus
6.5.1.9 cyclic 2,3-diphosphoglycerate synthetase
-
 Methanothermus fervidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.16 7
-
-
 Methanothermus fervidus
6.5.1.9 75 80
-
 Methanothermus fervidus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.16 108
-
 ATP pH 7.5, 75°C Methanothermus fervidus
6.5.1.9 13
-
 ATP at pH 7.0 and 75°C Methanothermus fervidus
6.5.1.9 13
-
 2,3-diphospho-D-glycerate at pH 7.0 and 75°C Methanothermus fervidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.16 6 8
-
 Methanothermus fervidus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.5.1.9 5 7.5 the enzyme is active between pH 5.0 and 7.5 Methanothermus fervidus

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.9 ATP
-
 Methanothermus fervidus