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Literature summary extracted from

  • Mueller, U.; Buckel, W.
    Activation of (R)-2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans (1995), Eur. J. Biochem., 230, 698-704 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.1.9 expression in Escherichia coli Acidaminococcus fermentans

General Stability

EC Number General Stability Organism
5.6.1.9 glycerol, Fe2+, and several reducing agents in the purification buffers has no stabilizing effect Acidaminococcus fermentans
5.6.1.9 recombinant protein is very unstable, the use of proteinase inhibitors, Acidaminococcus fermentans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.1.9 soluble
-
 Acidaminococcus fermentans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.1.9 Iron HgdC contains 4 mol non-heme iron and 3 mol inorganic sulfur per mol Acidaminococcus fermentans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.1.9 55000
-
 gel filtration Acidaminococcus fermentans

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.9 Acidaminococcus fermentans P11568
-
-
5.6.1.9 Acidaminococcus fermentans DSM 20731 P11568
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
5.6.1.9 under air, the activator has a half-life of seconds and even under strict anaerobic conditions it is very unstable Acidaminococcus fermentans

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.9
-
 Acidaminococcus fermentans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6.1.9 2000
-
 pH not specified in the publication, temperature not specified in the publication Acidaminococcus fermentans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.9 2 ATP + a reduced flavodoxin + inactive 2-hydroxyglutaryl-CoA dehydratase + 2 H2O only catalytic amounts of activator HgdC are required for complete activation of the dehydratase Acidaminococcus fermentans 2 ADP + 2 phosphate + a flavodoxin semiquinone + active 2-hydroxyglutaryl-CoA dehydratase
-
 ?
5.6.1.9 2 ATP + a reduced flavodoxin + inactive 2-hydroxyglutaryl-CoA dehydratase + 2 H2O only catalytic amounts of activator HgdC are required for complete activation of the dehydratase Acidaminococcus fermentans DSM 20731 2 ADP + 2 phosphate + a flavodoxin semiquinone + active 2-hydroxyglutaryl-CoA dehydratase
-
 ?

Subunits

EC Number Subunits Comment Organism
5.6.1.9 dimer 2 27500, calculated from sequence Acidaminococcus fermentans

Synonyms

EC Number Synonyms Comment Organism
5.6.1.9 HDC
-
 Acidaminococcus fermentans