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Literature summary extracted from

  • Schweiger, G.; Dutscho, R.; Buckel, W.
    Purification of 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans. An iron-sulfur protein (1987), Eur. J. Biochem., 169, 441-448 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.1.9 ATP inhibitory above 0.15 mM. 40% inhibition at 0.6 mM Acidaminococcus fermentans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.1.9 50000 60000 gel filtration Acidaminococcus fermentans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.1.9 2 ATP + a reduced flavodoxin + inactive 2-hydroxyglutaryl-CoA dehydratase + 2 H2O Acidaminococcus fermentans
-
 2 ADP + 2 phosphate + a flavodoxin semiquinone + active 2-hydroxyglutaryl-CoA dehydratase
-
 ?
5.6.1.9 2 ATP + a reduced flavodoxin + inactive 2-hydroxyglutaryl-CoA dehydratase + 2 H2O Acidaminococcus fermentans DSM 20731
-
 2 ADP + 2 phosphate + a flavodoxin semiquinone + active 2-hydroxyglutaryl-CoA dehydratase
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.9 Acidaminococcus fermentans P11568
-
-
5.6.1.9 Acidaminococcus fermentans DSM 20731 P11568
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
5.6.1.9 the activator protein is irreversibly inactivated by exposure to air within less than 15 min Acidaminococcus fermentans

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.9
-
 Acidaminococcus fermentans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.9 2 ATP + a reduced flavodoxin + inactive 2-hydroxyglutaryl-CoA dehydratase + 2 H2O
-
 Acidaminococcus fermentans 2 ADP + 2 phosphate + a flavodoxin semiquinone + active 2-hydroxyglutaryl-CoA dehydratase
-
 ?
5.6.1.9 2 ATP + a reduced flavodoxin + inactive 2-hydroxyglutaryl-CoA dehydratase + 2 H2O
-
 Acidaminococcus fermentans DSM 20731 2 ADP + 2 phosphate + a flavodoxin semiquinone + active 2-hydroxyglutaryl-CoA dehydratase
-
 ?
5.6.1.9 additional information the activation of the dehydratase requires only ATP Acidaminococcus fermentans ?
-
 ?
5.6.1.9 additional information the activation of the dehydratase requires only ATP Acidaminococcus fermentans DSM 20731 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.6.1.9 dimer 2 * 25000, SDS-PAGE Acidaminococcus fermentans