EC Number | Application | Comment | Organism |
---|---|---|---|
3.5.1.1 | analysis | assembly a microplate-based biosensor for the determination of L-asparagine in biological samples. The enzyme is immobilized by crosslinking with glutaraldehyde in a microplate in 96-well format. The sensing is based on the colorimetric measurement of ammonia formation using the Nessler's reagent. The sensor enables monitoring of L-asparagine levels in serum and foods samples in the concentration range 10-200 microM, with a detection limit of 10 microM for L-asparagine | Pectobacterium carotovorum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.1 | 0.07 | - |
L-asparagine | pH 8.0, 25°C | Pectobacterium carotovorum | |
3.5.1.1 | 0.3 | - |
succinamic acid | pH 8.0, 25°C | Pectobacterium carotovorum | |
3.5.1.1 | 0.5 | - |
Nalpha-acetyl-L-asparagine | pH 8.0, 25°C | Pectobacterium carotovorum | |
3.5.1.1 | 5.2 | - |
L-glutamine | pH 8.0, 25°C | Pectobacterium carotovorum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.1 | Pectobacterium carotovorum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.1 | single-step procedure | Pectobacterium carotovorum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.1 | L-asparagine + H2O | - |
Pectobacterium carotovorum | L-aspartate + NH3 | - |
? | |
3.5.1.1 | L-glutamine + H2O | reaction of EC 3.5.1.2 | Pectobacterium carotovorum | L-glutamate + NH3 | - |
? | |
3.5.1.1 | additional information | no substrate: D-asparagine, beta-alanine amide | Pectobacterium carotovorum | ? | - |
? | |
3.5.1.1 | Nalpha-acetyl-L-asparagine + H2O | - |
Pectobacterium carotovorum | Nalpha-acetyl-L-aspartate + NH3 | - |
? | |
3.5.1.1 | succinamic acid + H2O | - |
Pectobacterium carotovorum | succinate + NH3 | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.1 | 0.42 | - |
L-glutamine | pH 8.0, 25°C | Pectobacterium carotovorum | |
3.5.1.1 | 4.1 | - |
succinamic acid | pH 8.0, 25°C | Pectobacterium carotovorum | |
3.5.1.1 | 4.1 | - |
Nalpha-acetyl-L-asparagine | pH 8.0, 25°C | Pectobacterium carotovorum | |
3.5.1.1 | 55.2 | - |
L-asparagine | pH 8.0, 25°C | Pectobacterium carotovorum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.1 | 0.42 | - |
L-glutamine | pH 8.0, 25°C | Pectobacterium carotovorum | |
3.5.1.1 | 8.2 | - |
Nalpha-acetyl-L-asparagine | pH 8.0, 25°C | Pectobacterium carotovorum | |
3.5.1.1 | 13.7 | - |
succinamic acid | pH 8.0, 25°C | Pectobacterium carotovorum | |
3.5.1.1 | 788.6 | - |
L-asparagine | pH 8.0, 25°C | Pectobacterium carotovorum |