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Literature summary extracted from

  • Labrou, N.E.; Muharram, M.M.
    Biochemical characterization and immobilization of Erwinia carotovora l-asparaginase in a microplate for high-throughput biosensing of L-asparagine (2016), Enzyme Microb. Technol., 92, 86-93 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.1 analysis assembly a microplate-based biosensor for the determination of L-asparagine in biological samples. The enzyme is immobilized by crosslinking with glutaraldehyde in a microplate in 96-well format. The sensing is based on the colorimetric measurement of ammonia formation using the Nessler's reagent. The sensor enables monitoring of L-asparagine levels in serum and foods samples in the concentration range 10-200 microM, with a detection limit of 10 microM for L-asparagine Pectobacterium carotovorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 0.07
-
L-asparagine pH 8.0, 25°C Pectobacterium carotovorum
3.5.1.1 0.3
-
succinamic acid pH 8.0, 25°C Pectobacterium carotovorum
3.5.1.1 0.5
-
Nalpha-acetyl-L-asparagine pH 8.0, 25°C Pectobacterium carotovorum
3.5.1.1 5.2
-
L-glutamine pH 8.0, 25°C Pectobacterium carotovorum

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Pectobacterium carotovorum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 single-step procedure Pectobacterium carotovorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
Pectobacterium carotovorum L-aspartate + NH3
-
?
3.5.1.1 L-glutamine + H2O reaction of EC 3.5.1.2 Pectobacterium carotovorum L-glutamate + NH3
-
?
3.5.1.1 additional information no substrate: D-asparagine, beta-alanine amide Pectobacterium carotovorum ?
-
?
3.5.1.1 Nalpha-acetyl-L-asparagine + H2O
-
Pectobacterium carotovorum Nalpha-acetyl-L-aspartate + NH3
-
?
3.5.1.1 succinamic acid + H2O
-
Pectobacterium carotovorum succinate + NH3
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.1 0.42
-
L-glutamine pH 8.0, 25°C Pectobacterium carotovorum
3.5.1.1 4.1
-
succinamic acid pH 8.0, 25°C Pectobacterium carotovorum
3.5.1.1 4.1
-
Nalpha-acetyl-L-asparagine pH 8.0, 25°C Pectobacterium carotovorum
3.5.1.1 55.2
-
L-asparagine pH 8.0, 25°C Pectobacterium carotovorum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.1 0.42
-
L-glutamine pH 8.0, 25°C Pectobacterium carotovorum
3.5.1.1 8.2
-
Nalpha-acetyl-L-asparagine pH 8.0, 25°C Pectobacterium carotovorum
3.5.1.1 13.7
-
succinamic acid pH 8.0, 25°C Pectobacterium carotovorum
3.5.1.1 788.6
-
L-asparagine pH 8.0, 25°C Pectobacterium carotovorum