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Literature summary extracted from

  • Ulrich, B.; Palatucci, M.; Bolotin, J.; Spain, J.; Hofstetter, T.
    Different mechanisms of alkaline and enzymatic hydrolysis of the insensitive munition component 2,4-dinitroanisole lead to identical products (2018), Environ. Sci. Technol. Lett., 5, 456-461 .
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.3.2.14 2,4-dinitroanisole + H2O Nocardioides sp. JS1661
-
 methanol + 2,4-dinitrophenol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.14 Nocardioides sp. JS1661 A0A096ZEC9 AND A0A096ZED0 subunit alpha and subunit beta, encoded by genes dnhA and dnhB
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.3.2.14 native enzyme from strain JS1661 by ammonium sulfate fractionation, hydrophobic interaction chromatography, and ultrafiltration Nocardioides sp. JS1661

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.14 2,4-dinitroanisole + H2O
-
 Nocardioides sp. JS1661 methanol + 2,4-dinitrophenol
-
 ?
3.3.2.14 2,4-dinitroanisole + H2O stoichiometric formation of 2,4-dinitrophenol Nocardioides sp. JS1661 methanol + 2,4-dinitrophenol
-
 ?
3.3.2.14 additional information alkaline hydrolysis of DNAN is associated with considerable C and N isotope fractionation, isotopic analyses by gas chromatography and isotope ratio mass spectrometry (GC/IRMS). Carbon and nitrogen isotope enrichment factors (epsilonC and epsilonN), apparent 13C and 15N kinetic isotope effects, and correlations of C and N isotope fractionation (DELTAN/C) associated with the alkaline and enzymatic hydrolysis of DNAN, overview Nocardioides sp. JS1661 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.3.2.14 DNAN O-demethylase
-
 Nocardioides sp. JS1661

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.3.2.14 25
-
 assay at Nocardioides sp. JS1661

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.3.2.14 7
-
 assay at, intact cells Nocardioides sp. JS1661
3.3.2.14 7.5
-
 assay at, partially purified enzyme Nocardioides sp. JS1661

General Information

EC Number General Information Comment Organism
3.3.2.14 additional information evaluation of the C and N isotope fractionation associated with abiotic and biological 2,4-dinitroanisole (DNAN) hydrolysis through alkaline hydrolysis at high pH as well as enzymatic hydrolysis by Nocardioides sp. JS1661 and partially purified DNAN O-demethylase for decontamination of DNAN. Whereas both reactions generate 2,4-dinitrophenol (DNP), compound-specific isotope analysis (CSIA) of DNAN and DNP reveal that these reactions occur by different mechanisms, thus different mechanisms of alkaline and enzymatic hydrolysis of the insensitive munition component 2,4-dinitroanisole lead to identical products Nocardioides sp. JS1661