Literature summary extracted from
Karthikeyan, S.; Kurt, Z.; Pandey, G.; Spain, J.C.
Immobilized biocatalyst for detection and destruction of the insensitive explosive, 2,4-dinitroanisole (DNAN) (2016), Environ. Sci. Technol., 50, 11193-11199 .
Application
EC Number |
Application |
Comment |
Organism |
---|
3.3.2.14 |
environmental protection |
the immobilized enzyme can be used as biocatalyst for detection and destruction of the insensitive explosive, 2,4-dinitroanisole (DNAN), with a wide spectrum of applications ranging from national security and demilitarization to environmental monitoring and restoration |
Nocardioides sp. JS1661 |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.3.2.14 |
additional information |
enzyme immobilization by biogenic silica encapsulation which is used to stabilize the enzyme and to enable it to be packed into a model microcolumn for application as a biosensor or as a bioreactor for continuous destruction of 2,4-dinitroanisole (DNAN). The immobilized enzyme is stable and not inhibited by other insensitive munitions constituents. The highly specific, convenient and robust biocatalyst DNAN demethylase can mineralize DNAN. An alternative method for DNAN detection involved coating the encapsulated enzyme on cellulose filter paper. Methods, detailed overview |
Nocardioides sp. JS1661 |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.3.2.14 |
2,4-dinitroanisole + H2O |
Nocardioides sp. JS1661 |
- |
methanol + 2,4-dinitrophenol |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.3.2.14 |
Nocardioides sp. JS1661 |
A0A096ZEC9 AND A0A096ZED0 |
subunit alpha and subunit beta, encoded by genes dnhA and dnhB |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.3.2.14 |
native enzyme from strain JS1661 by ammonium sulfate fractionation, hydrophobic interaction chromatography, and ultrafiltration |
Nocardioides sp. JS1661 |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.3.2.14 |
2,4-dinitroanisole + H2O |
- |
Nocardioides sp. JS1661 |
methanol + 2,4-dinitrophenol |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.3.2.14 |
DNAN O-demethylase |
- |
Nocardioides sp. JS1661 |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.3.2.14 |
22 |
- |
assay at room temperature |
Nocardioides sp. JS1661 |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.3.2.14 |
8 |
- |
assay at |
Nocardioides sp. JS1661 |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
3.3.2.14 |
additional information |
the enzyme requires no cofactors |
Nocardioides sp. JS1661 |
|