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Literature summary extracted from

  • Zhao, T.; Zhang, Y.; Wu, H.; Wang, D.; Chen, Y.; Zhu, M.-J.; Ma, L.Z.
    Extracellular aminopeptidase modulates biofilm development of Pseudomonas aeruginosa by affecting matrix exopolysaccharide and bacterial cell death (2018), Environ. Microbiol. Rep., 10, 583-593 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.11.1 medicine enzyme PaAP is a therapeutic target for preventing Pseudomonas aeruginosa infection and combating biofilm-related complications Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.1 gene paaP, cloning in Escherichia coli strain DH5alpha Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.1 additional information construction of an enzyme deletion mutant DELTApaaP Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.1 extracellular
-
 Pseudomonas aeruginosa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Ca2+ required Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Pseudomonas aeruginosa Q9HZQ8
-
-
3.4.11.1 Pseudomonas aeruginosa 1C Q9HZQ8
-
-
3.4.11.1 Pseudomonas aeruginosa ATCC 15692 Q9HZQ8
-
-
3.4.11.1 Pseudomonas aeruginosa CIP 104116 Q9HZQ8
-
-
3.4.11.1 Pseudomonas aeruginosa DSM 22644 Q9HZQ8
-
-
3.4.11.1 Pseudomonas aeruginosa JCM 14847 Q9HZQ8
-
-
3.4.11.1 Pseudomonas aeruginosa LMG 12228 Q9HZQ8
-
-
3.4.11.1 Pseudomonas aeruginosa PRS 101 Q9HZQ8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 L-Leu-4-nitroanilide + H2O
-
 Pseudomonas aeruginosa L-Leu + 4-nitroaniline
-
 ?
3.4.11.1 L-Leu-4-nitroanilide + H2O
-
 Pseudomonas aeruginosa ATCC 15692 L-Leu + 4-nitroaniline
-
 ?
3.4.11.1 L-Leu-4-nitroanilide + H2O
-
 Pseudomonas aeruginosa 1C L-Leu + 4-nitroaniline
-
 ?
3.4.11.1 L-Leu-4-nitroanilide + H2O
-
 Pseudomonas aeruginosa PRS 101 L-Leu + 4-nitroaniline
-
 ?
3.4.11.1 L-Leu-4-nitroanilide + H2O
-
 Pseudomonas aeruginosa DSM 22644 L-Leu + 4-nitroaniline
-
 ?
3.4.11.1 L-Leu-4-nitroanilide + H2O
-
 Pseudomonas aeruginosa CIP 104116 L-Leu + 4-nitroaniline
-
 ?
3.4.11.1 L-Leu-4-nitroanilide + H2O
-
 Pseudomonas aeruginosa LMG 12228 L-Leu + 4-nitroaniline
-
 ?
3.4.11.1 L-Leu-4-nitroanilide + H2O
-
 Pseudomonas aeruginosa JCM 14847 L-Leu + 4-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 LAP
-
 Pseudomonas aeruginosa
3.4.11.1 PA2939
-
 Pseudomonas aeruginosa
3.4.11.1 PaAP
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 50
-
-
 Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 8.3
-
-
 Pseudomonas aeruginosa

Expression

EC Number Organism Comment Expression
3.4.11.1 Pseudomonas aeruginosa enzyme PaAP is a quorum sensing-regulated protein, thus its expression is largely activated at high bacterial density up

General Information

EC Number General Information Comment Organism
3.4.11.1 malfunction lack of extracellular PaAP in biofilms leading to bacterial cell death implies that PaAP might function as a nutrient recycler within a biofilm. Biomass reduction of mutant DELTApaaP grown under a continuous media flow has one day delay compared with the DELTApaaP pellicles grown on static media. Lack of extracellular PaAP triggers bacterial cell death and bacterial cell death in DELTApaaP biofilms is independent of Psl, while the disruption of DELTApaaP biofilms is a result of the degradation of Psl matrix by PslG released from the dead bacteria within biofilms, which in turn led to the dispersion of live bacteria from biofilms into effluents Pseudomonas aeruginosa
3.4.11.1 physiological function the extracellular aminopeptidase PaAP can release amino acids from the N-terminus of peptide or protein. It modulates biofilm development of Pseudomonas aeruginosa by affecting matrix exopolysaccharide Psl and bacterial cell death. PaAP is a quorum sensing-regulated protein, thus its expression is largely activated at high bacterial density. PaAP might function as a nutrient recycler within a biofilm Pseudomonas aeruginosa