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Literature summary extracted from

  • Fu, Z.; Akula, S.; Thorpe, M.; Chahal, G.; de Garavilla, L.; Kervinen, J.; Hellman, L.
    Extended cleavage specificity of sheep mast cell protease-2 A classical chymase with preference to aromatic P1 substrate residues (2019), Dev. Comp. Immunol., 92, 160-169 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.39 enterokinase
-
 Ovis aries

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.39 expressed in baculovirus-infected insect cells Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.39 Ovis aries P79204
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.39 heparin-Sepharose column chromatography and phenyl HP column chromatography Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.39 mast cell
-
 Ovis aries
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.39 additional information no activity with succinyl-AAPI-4-nitroanilide, succinyl-AAPA-4-nitroanilide, succinyl-AAPV-4-nitroanilide, succinyl-VLGR-4-nitroanilide, benzyloxycarbonyl-GPR-4-nitroanilide, acetyl-YVAD-4-nitroanilide, acetyl-VEID-4-nitroanilide, and acetyl-IEPD-4-nitroanilide Ovis aries ?
-
 ?
3.4.21.39 additional information the enzyme shows preference for aromatic residues (Phe, Tyr, Trp) in the P1 position of substrates and a preference for aliphatic residues in most other substrate positions around the cleavage site. The enzyme also cleaves, albeit with relatively low efficiency, after Leu in the P1 position and exhibits unspecific preference in the P2' position Ovis aries ?
-
 ?
3.4.21.39 succinyl-AAPF-4-nitroanilide + H2O
-
 Ovis aries succinyl-AAPF + 4-nitroaniline
-
 ?
3.4.21.39 succinyl-AAPL-4-nitroanilide + H2O low activity Ovis aries succinyl-AAPL + 4-nitroaniline
-
 ?
3.4.21.39 succinyl-LLVY-4-nitroanilide + H2O
-
 Ovis aries succinyl-LLVY + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.39 ? x * 26000, SDS-PAGE Ovis aries

Synonyms

EC Number Synonyms Comment Organism
3.4.21.39 mast cell protease-2
-
 Ovis aries
3.4.21.39 MCP2
-
 Ovis aries