EC Number | Application | Comment | Organism |
---|---|---|---|
3.5.1.1 | food industry | addition of partially purified L-asparaginase to potato products followed by incubation of the mixture at 37°C for 30 min leads to 92% reduction of acrylamide content | Penicillium cyclopium |
3.5.1.1 | medicine | the purified enzyme inhibits the growth of human cell lines Hep-G2, MCF-7 and PC-3 with IC50 values of 14 microg/ml, 12.5 microg/ml and 37 microg/ml, respectively | Penicillium cyclopium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.1 | EDTA | 3 mM, 45.8% residual activity | Penicillium cyclopium | |
3.5.1.1 | Fe3+ | 3 mM, 50.3% residual activity | Penicillium cyclopium | |
3.5.1.1 | Hg2+ | 3 mM, no residual activity | Penicillium cyclopium | |
3.5.1.1 | Mn2+ | 3 mM, 60.4% residual activity | Penicillium cyclopium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.1 | 0.3 | - |
L-asparagine | pH 8.0, 37°C | Penicillium cyclopium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.1 | Penicillium cyclopium | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.1 | L-asparagine + H2O | - |
Penicillium cyclopium | L-aspartate + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.1 | ? | x * 55000, SDS-PAGE | Penicillium cyclopium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.1 | 37 | - |
- |
Penicillium cyclopium |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.1 | 30 | - |
30 min, no loss of activity | Penicillium cyclopium |
3.5.1.1 | 50 | - |
60 min, about 25% loss of activity | Penicillium cyclopium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.1 | 8 | - |
- |
Penicillium cyclopium |