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Literature summary extracted from

  • Roy, S.; Biswas, S.
    Asp72 of pro-peptide is an important pH sensor in the zymogen activation process of papain A structural and mechanistic insight (2018), Curr. Sci., 114, 2356-2362 .
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.22.2
-
 Carica papaya

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.2 D72A the mutant undergoes autoactivation at pH 5.0 compared to pH 4.0 for wild-type enzyme. The general proteolytic activity of the D72A mutant remains similar to that of wild-type Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya P00784
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.2 proteolytic modification the zymogen of papain contains a pro-peptide at the N-terminus of the catalytic domain. Pro-peptide contains residues which act as pH-sensors in zymogen activation cascade. The conserved Asp72 residue of the pro-peptide, is a highly conserved residue of the GXNXFXD motif that is an important pH sensor in the zymogen activation process and the pro-peptide part can also be a useful target of protein engineering for altering the activation pH of a protease Carica papaya