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Literature summary extracted from

  • Fang, J.; Feng, Q.; Ketel, C.S.; Wang, H.; Cao, R.; Xia, L.; Erdjument-Bromage, H.; Tempst, P.; Simon, J.A.; Zhang, Y.
    Purification and functional characterization of SET8, a nucleosomal histone H4-lysine 20-specific methyltransferase (2002), Curr. Biol., 12, 1086-1099 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.361
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.361 H299A the mutation greatly impairs the enzymatic activity toward nucleosomal histone H4 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.361 nucleosome
-
 Homo sapiens 786
-
2.1.1.361 nucleosome
-
 Drosophila melanogaster 786
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.361 66000 150000 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.361 additional information Homo sapiens the enzyme is completely inactive toward histone H4 with a K20L mutation ?
-
 ?
2.1.1.361 additional information Drosophila melanogaster the enzyme is completely inactive toward histone H4 with a K20L mutation ?
-
 ?
2.1.1.361 S-adenosyl-L-methionine + a [histone H4]-L-lysine20 Homo sapiens highly specific substrate S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20
-
 ?
2.1.1.361 S-adenosyl-L-methionine + a [histone H4]-L-lysine20 Drosophila melanogaster highly specific substrate S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.361 Drosophila melanogaster Q9VFK6
-
-
2.1.1.361 Homo sapiens Q9NQR1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.361 DEAE52 column chromatography, phosphocellulose P11 column chromatography, DEAE-5PW column chromatography, and hydroxyapatite column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.361 HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.361 additional information the enzyme is completely inactive toward histone H4 with a K20L mutation Homo sapiens ?
-
 ?
2.1.1.361 additional information the enzyme is completely inactive toward histone H4 with a K20L mutation Drosophila melanogaster ?
-
 ?
2.1.1.361 S-adenosyl-L-methionine + a [histone H4]-L-lysine20 highly specific substrate Homo sapiens S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20
-
 ?
2.1.1.361 S-adenosyl-L-methionine + a [histone H4]-L-lysine20 highly specific substrate Drosophila melanogaster S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.361 homodimer 2 * 45000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.1.1.361 CG3307
-
 Drosophila melanogaster
2.1.1.361 H4-K20-specific HMTase
-
 Homo sapiens
2.1.1.361 H4-K20-specific HMTase
-
 Drosophila melanogaster
2.1.1.361 H4-K20-specific methyltransferase
-
 Homo sapiens
2.1.1.361 H4-K20-specific methyltransferase
-
 Drosophila melanogaster
2.1.1.361 histone H4-lysine 20-specific methyltransferase
-
 Homo sapiens
2.1.1.361 histone H4-lysine 20-specific methyltransferase
-
 Drosophila melanogaster
2.1.1.361 SET8
-
 Homo sapiens
2.1.1.361 SET8
-
 Drosophila melanogaster

General Information

EC Number General Information Comment Organism
2.1.1.361 malfunction enzyme gene disruption results in lethality Drosophila melanogaster
2.1.1.361 physiological function the enzyme is essential for Drosophila development Drosophila melanogaster