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Literature summary extracted from
- Penicillin acylases revisited Importance beyond their industrial utility (2016), Crit. Rev. Biotechnol., 36, 303-316 .
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.11 | 0.0197 | - |
6-nitro-3-(phenylacetamido)-benzoic acid | pH and temperature not specified in the publication | Providencia rettgeri |  |
| 3.5.1.11 | 0.42 | - |
penicillin G | pH and temperature not specified in the publication | Rhizobium viscosum | |
| 3.5.1.11 | 1.83 | - |
penicillin G | pH and temperature not specified in the publication | Priestia megaterium | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.5.1.11 | cytoplasm | - |
Lysinibacillus sphaericus | 5737 | - |
| 3.5.1.11 | cytoplasm | - |
Achromobacter sp. | 5737 | - |
| 3.5.1.11 | extracellular | - |
Rhizobium viscosum | - |
- |
| 3.5.1.11 | intracellular | - |
Bacillus badius | 5622 | - |
| 3.5.1.11 | periplasm | - |
Achromobacter xylosoxidans | - |
- |
| 3.5.1.11 | periplasm | - |
Escherichia coli | - |
- |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.11 | Achromobacter sp. | - |
- |
- |
| 3.5.1.11 | Achromobacter xylosoxidans | - |
- |
- |
| 3.5.1.11 | Alcaligenes faecalis | - |
- |
- |
| 3.5.1.11 | Bacillus badius | - |
- |
- |
| 3.5.1.11 | Bacillus subtilis | - |
- |
- |
| 3.5.1.11 | Escherichia coli | P06875 | - |
- |
| 3.5.1.11 | Fusarium oxysporum | - |
- |
- |
| 3.5.1.11 | Kluyvera cryocrescens | P07941 | - |
- |
| 3.5.1.11 | Lysinibacillus sphaericus | - |
- |
- |
| 3.5.1.11 | Priestia megaterium | Q60136 | - |
- |
| 3.5.1.11 | Providencia rettgeri | - |
- |
- |
| 3.5.1.11 | Rhizobium viscosum | P31956 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.11 | 6-nitro-3-(phenylacetamido)-benzoic acid + H2O | - |
Alcaligenes faecalis | 3-amino-6-nitrobenzoic acid + phenylacetate | - |
? | |
| 3.5.1.11 | 6-nitro-3-(phenylacetamido)-benzoic acid + H2O | - |
Achromobacter sp. | 3-amino-6-nitrobenzoic acid + phenylacetate | - |
? | |
| 3.5.1.11 | 6-nitro-3-(phenylacetamido)-benzoic acid + H2O | - |
Achromobacter xylosoxidans | 3-amino-6-nitrobenzoic acid + phenylacetate | - |
? | |
| 3.5.1.11 | 6-nitro-3-(phenylacetamido)-benzoic acid + H2O | - |
Providencia rettgeri | 3-amino-6-nitrobenzoic acid + phenylacetate | - |
? | |
| 3.5.1.11 | 6-nitro-3-(phenylacetamido)-benzoic acid + H2O | - |
Bacillus badius | 3-amino-6-nitrobenzoic acid + phenylacetate | - |
? | |
| 3.5.1.11 | 6-nitro-3-(phenylacetamido)-benzoic acid + H2O | - |
Escherichia coli | 3-amino-6-nitrobenzoic acid + phenylacetate | - |
? | |
| 3.5.1.11 | 6-nitro-3-(phenylacetamido)-benzoic acid + H2O | - |
Kluyvera cryocrescens | 3-amino-6-nitrobenzoic acid + phenylacetate | - |
? | |
| 3.5.1.11 | ampicillin + H2O | - |
Bacillus badius | 6-aminopenicillanic acid + (R)-2-phenylglycine | - |
? | |
| 3.5.1.11 | ampicillin + H2O | - |
Achromobacter sp. | (R)-2-phenylglycine + 6-aminopenicillanic acid | - |
? | |
| 3.5.1.11 | cephalexin + H2O | - |
Achromobacter sp. | 7-aminodesacetoxycephalosporanic acid + D-phenylglycine | - |
? | |
| 3.5.1.11 | cephalexin + H2O | - |
Bacillus badius | 7-aminodesacetoxycephalosporanic acid + D-phenylglycine | - |
? | |
| 3.5.1.11 | cephalothin + H2O | - |
Bacillus badius | (6R,7R)-3-[(acetyloxy)methyl]-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (thiophen-2-yl)acetic acid | - |
? | |
| 3.5.1.11 | penicillin G + H2O | - |
Alcaligenes faecalis | 6-aminopenicillanic acid + phenylacetic acid | - |
? | |
| 3.5.1.11 | penicillin G + H2O | - |
Achromobacter sp. | 6-aminopenicillanic acid + phenylacetic acid | - |
? | |
| 3.5.1.11 | penicillin G + H2O | - |
Achromobacter xylosoxidans | 6-aminopenicillanic acid + phenylacetic acid | - |
? | |
| 3.5.1.11 | penicillin G + H2O | - |
Providencia rettgeri | 6-aminopenicillanic acid + phenylacetic acid | - |
? | |
| 3.5.1.11 | penicillin G + H2O | - |
Bacillus badius | 6-aminopenicillanic acid + phenylacetic acid | - |
? | |
| 3.5.1.11 | penicillin G + H2O | - |
Priestia megaterium | 6-aminopenicillanic acid + phenylacetic acid | - |
? | |
| 3.5.1.11 | penicillin G + H2O | - |
Escherichia coli | 6-aminopenicillanic acid + phenylacetic acid | - |
? | |
| 3.5.1.11 | penicillin G + H2O | - |
Kluyvera cryocrescens | 6-aminopenicillanic acid + phenylacetic acid | - |
? | |
| 3.5.1.11 | penicillin G + H2O | - |
Rhizobium viscosum | 6-aminopenicillanic acid + phenylacetic acid | - |
? | |
| 3.5.1.11 | penicillin V + H2O | - |
Lysinibacillus sphaericus | phenoxyacetate + 6-aminopenicillinate | - |
? | |
| 3.5.1.11 | penicillin V + H2O | - |
Fusarium oxysporum | phenoxyacetate + 6-aminopenicillinate | - |
? | |
| 3.5.1.11 | penicillin V + H2O | specific for penicillin V | Bacillus subtilis | phenoxyacetate + 6-aminopenicillinate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.11 | penicillin G acylase | - |
Alcaligenes faecalis |
| 3.5.1.11 | penicillin G acylase | - |
Achromobacter sp. |
| 3.5.1.11 | penicillin G acylase | - |
Achromobacter xylosoxidans |
| 3.5.1.11 | penicillin G acylase | - |
Providencia rettgeri |
| 3.5.1.11 | penicillin G acylase | - |
Bacillus badius |
| 3.5.1.11 | penicillin G acylase | - |
Priestia megaterium |
| 3.5.1.11 | penicillin G acylase | - |
Escherichia coli |
| 3.5.1.11 | penicillin G acylase | - |
Kluyvera cryocrescens |
| 3.5.1.11 | penicillin G acylase | - |
Rhizobium viscosum |
| 3.5.1.11 | penicillin V acylase | - |
Bacillus subtilis |
| 3.5.1.11 | penicillin V acylase | - |
Lysinibacillus sphaericus |
| 3.5.1.11 | penicillin V acylase | - |
Fusarium oxysporum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.11 | additional information | - |
thermostable enzyme | Alcaligenes faecalis |
| 3.5.1.11 | additional information | - |
thermostable enzyme | Achromobacter xylosoxidans |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.5.1.11 | physiological function | possible scavenging of phenylacetyl compounds, cell signalling | Escherichia coli |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.11 | 3 | - |
Penicillin V | pH and temperature not specified in the publication | Bacillus subtilis | |
| 3.5.1.11 | 4.1 | - |
Penicillin V | pH and temperature not specified in the publication | Lysinibacillus sphaericus | |
| 3.5.1.11 | 5.2 | - |
Penicillin V | pH and temperature not specified in the publication | Fusarium oxysporum | |
| 3.5.1.11 | 21 | - |
ampicillin | pH and temperature not specified in the publication | Bacillus badius | |
| 3.5.1.11 | 58.2 | - |
cephalexin | pH and temperature not specified in the publication | Bacillus badius | |
| 3.5.1.11 | 86 | - |
cephalothin | pH and temperature not specified in the publication | Bacillus badius | |
| 3.5.1.11 | 805 | - |
6-nitro-3-(phenylacetamido)-benzoic acid | pH and temperature not specified in the publication | Bacillus badius | |
| 3.5.1.11 | 1025 | - |
penicillin G | pH and temperature not specified in the publication | Bacillus badius | |
| 3.5.1.11 | 1700 | - |
6-nitro-3-(phenylacetamido)-benzoic acid | pH and temperature not specified in the publication | Escherichia coli | |
| 3.5.1.11 | 2500 | - |
6-nitro-3-(phenylacetamido)-benzoic acid | pH and temperature not specified in the publication | Kluyvera cryocrescens | |
| 3.5.1.11 | 2600 | - |
6-nitro-3-(phenylacetamido)-benzoic acid | pH and temperature not specified in the publication | Achromobacter xylosoxidans | |
| 3.5.1.11 | 3100 | - |
penicillin G | pH and temperature not specified in the publication | Kluyvera cryocrescens | |
| 3.5.1.11 | 4200 | - |
6-nitro-3-(phenylacetamido)-benzoic acid | pH and temperature not specified in the publication | Achromobacter sp. | |
| 3.5.1.11 | 8200 | - |
penicillin G | pH and temperature not specified in the publication | Achromobacter xylosoxidans | |
| 3.5.1.11 | 10000 | - |
penicillin G | pH and temperature not specified in the publication | Escherichia coli | |
| 3.5.1.11 | 13000 | - |
penicillin G | pH and temperature not specified in the publication | Alcaligenes faecalis | |
| 3.5.1.11 | 15000 | - |
penicillin G | pH and temperature not specified in the publication | Achromobacter sp. | |
| 3.5.1.11 | 21000 | - |
6-nitro-3-(phenylacetamido)-benzoic acid | pH and temperature not specified in the publication | Alcaligenes faecalis | |
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