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Literature summary extracted from
- Pepsinogens and pepsins from largemouth bass, Micropterus salmoides purification and characterization with special reference to high proteolytic activities of bass enzymes (2015), Comp. Biochem. Physiol. B, 183, 42-48 .
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.1 | pepstatin | - |
Micropterus salmoides |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.1 | 0.02 | - |
hemoglobin | isoform PG2-2, at pH 3.0 and 37°C | Micropterus salmoides | |
| 3.4.23.1 | 0.0344 | - |
Substance P | isoform PG2-2, at pH 3.0 and 37°C | Micropterus salmoides | |
| 3.4.23.1 | 0.039 | - |
hemoglobin | isoform PG1-1, at pH 3.0 and 37°C | Micropterus salmoides | |
| 3.4.23.1 | 0.698 | - |
Substance P | isoform PG1-1, at pH 3.0 and 37°C | Micropterus salmoides | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.1 | Micropterus salmoides | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.23.1 | DEAE Sephacel column chromatography, Sephadex G-100 gel filtration, and Mono Q column chromatography | Micropterus salmoides |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.23.1 | gastric mucosa | - |
Micropterus salmoides | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.1 | Hemoglobin + H2O | - |
Micropterus salmoides | ? | - |
? | |
| 3.4.23.1 | oxidized insulin B chain + H2O | the cleavage specificities clarified with oxidized insulin B chain are restricted to a few bonds consisting of hydrophobic/aromatic residues, such as the Leu15-Tyr16, Phe24-Phe25 and Phe25-Tyr26 bonds | Micropterus salmoides | FVNQHLCGSHLVEAL + YLVCGERGFFYTPKA | - |
? | |
| 3.4.23.1 | Substance P + H2O | RPKPQQFFGLM | Micropterus salmoides | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.23.1 | ? | x * 39000, isoform PG1-1, SDS-PAGE | Micropterus salmoides |
| 3.4.23.1 | ? | x * 41000, isoform PG2-2, SDS-PAGE | Micropterus salmoides |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.23.1 | PG1-1 | isoform | Micropterus salmoides |
| 3.4.23.1 | PG2-2 | isoform | Micropterus salmoides |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.1 | 40 | - |
isoform PG2-2 | Micropterus salmoides |
| 3.4.23.1 | 50 | - |
isoform PG1-1 | Micropterus salmoides |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.1 | 38.8 | - |
Substance P | isoform PG2-2, at pH 3.0 and 37°C | Micropterus salmoides | |
| 3.4.23.1 | 52.7 | - |
hemoglobin | isoform PG1-1, at pH 3.0 and 37°C | Micropterus salmoides | |
| 3.4.23.1 | 61 | - |
Substance P | isoform PG1-1, at pH 3.0 and 37°C | Micropterus salmoides | |
| 3.4.23.1 | 62.9 | - |
hemoglobin | isoform PG2-2, at pH 3.0 and 37°C | Micropterus salmoides |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.1 | 1.5 | - |
isoform PG1-1, for hemoglobin digestion | Micropterus salmoides |
| 3.4.23.1 | 2 | - |
isoform PG2-2, for hemoglobin digestion | Micropterus salmoides |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.1 | 1.5 | 3.5 | highly active between pH 1.5-3.5 | Micropterus salmoides |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.1 | 2 | - |
at pH 2.0 and 14°C, isoform PG1-1 and PG2-2 are converted through intermediate forms into their respective pepsins. While the intermediate form of PG1-1 is rather stable, as the enzyme remained in that form for more than 60 min, the intermediate form of PG2-2 is converted to pepsin rapidly | Micropterus salmoides |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.1 | 91 | - |
Substance P | isoform PG1-1, at pH 3.0 and 37°C | Micropterus salmoides | |
| 3.4.23.1 | 1140 | - |
Substance P | isoform PG2-2, at pH 3.0 and 37°C | Micropterus salmoides | |
| 3.4.23.1 | 1600 | - |
hemoglobin | isoform PG1-1, at pH 3.0 and 37°C | Micropterus salmoides | |
| 3.4.23.1 | 3500 | - |
hemoglobin | isoform PG2-2, at pH 3.0 and 37°C | Micropterus salmoides |
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