EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.24.80 | gene MMP14, recombinant expression of wild-type enzyme, a soluble enzyme form, and different enzyme MT1-MMP domain constructs in MCF-7 cells, overview | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.24.80 | additional information | deletion of the MT1-MMP cytoplasmic tail or deletion of the hemopexin-like domain | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.80 | THP-1 | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.80 | 0.0151 | - |
(Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly-Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 | pH not specified in the publication, 37°C, recombinant wild-type membrane-bound enzyme | Homo sapiens | |
3.4.24.80 | 0.0186 | - |
(Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly-Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 | pH not specified in the publication, 37°C, recombinant soluble enzyme | Homo sapiens | |
3.4.24.80 | 0.0234 | - |
(Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly-Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 | pH not specified in the publication, 37°C, recombinant enzyme mutant lacking the C-terminal domain | Homo sapiens | |
3.4.24.80 | 0.0368 | - |
(Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly-Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 | pH not specified in the publication, 37°C, recombinant enzyme mutant lacking the HPX domain | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.24.80 | cell surface | - |
Homo sapiens | 9986 | - |
3.4.24.80 | membrane | - |
Homo sapiens | 16020 | - |
3.4.24.80 | additional information | quantitative assessment of MT1-MMP cell surface-associated proteolytic activity compared to soluble activity. MT1-MMP activity (kcat/KM) on the cell surface is 4.8fold lower compared with soluble MT1-MMP, with the effect largely manifested in kcat. Deletion of the MT1-MMP cytoplasmic tail enhances cell surface activity, with both kcat and KM values affected, while deletion of the hemopexin-like domain negatively impacts KM and increases kcat. Cell surface localization of MT1-MMP restricts substrate binding and protein-coupled motions (based on changes in both kcat and KM) for catalysis | Homo sapiens | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.80 | Zn2+ | a zinc-dependent endopeptidase | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.80 | Collagen + H2O | Homo sapiens | - |
? | - |
? | |
3.4.24.80 | proMMP-2 + H2O | Homo sapiens | - |
MMP-2 + pro-peptide | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.80 | Homo sapiens | P50281 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.4.24.80 | proteolytic modification | MT1-MMP is activated by incubation of the proMT-MMP with rhTrypsin-3 for 1 hr at 37°C | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.24.80 | mesenchymal stem cell | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.80 | (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly-Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 + H2O | triple-helical substrate fTHP-9 | Homo sapiens | (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly + Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 | - |
? | |
3.4.24.80 | Collagen + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.24.80 | dabcyl-Gly-Gly-Pro-Gln-Gly-Ile-Trp-Gly-Gln-Lys(fluorescein)-Ahx-Cys + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.24.80 | proMMP-2 + H2O | - |
Homo sapiens | MMP-2 + pro-peptide | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.24.80 | ? | x * 65000, pro-MT1-MMP, SDS-PAGE, x * 57000, mature sMT1-MMP, SDS-PAGE | Homo sapiens |
3.4.24.80 | More | MT1-MMP domain structure, overview | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.24.80 | MT1-MMP | - |
Homo sapiens |
3.4.24.80 | sMT1-MMP | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.80 | 37 | - |
assay at | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.80 | 0.14 | - |
(Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly-Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 | pH not specified in the publication, 37°C, recombinant wild-type membrane-bound enzyme | Homo sapiens | |
3.4.24.80 | 0.2 | - |
(Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly-Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 | pH not specified in the publication, 37°C, recombinant enzyme mutant lacking the HPX domain | Homo sapiens | |
3.4.24.80 | 0.21 | - |
(Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly-Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 | pH not specified in the publication, 37°C, recombinant enzyme mutant lacking the C-terminal domain | Homo sapiens | |
3.4.24.80 | 0.84 | - |
(Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly-Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 | pH not specified in the publication, 37°C, recombinant soluble enzyme | Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.4.24.80 | malfunction | deletion of the MT1-MMP cytoplasmic tail enhances cell surface activity, with both kcat and KM values affected, while deletion of the hemopexin-like domain negatively impacts KM and increases kcat | Homo sapiens |
3.4.24.80 | additional information | visualization of membrane-bound, active MT1-MMP is achieved by fluorescence resonance energy transfer (FRET) imaging of surface-anchored sensors. An initial MT1-MMP sensor was created using the Cys-Pro-Lys-Glu-Ser-Cys-Asn-Leu-Phe-Val-Leu-Lys-Asp sequence, derived from the MT1-MMP cleavage site in proMMP-2. The C-terminus plays a required role in regulating proteolytic activity at the cell surface | Homo sapiens |
3.4.24.80 | physiological function | cell surface localization of MT1-MMP restricts substrate binding and protein-coupled motions (based on changes in both kcat and KM) for catalysis | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.80 | 5.44 | - |
(Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly-Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 | pH not specified in the publication, 37°C, recombinant enzyme mutant lacking the HPX domain | Homo sapiens | |
3.4.24.80 | 8.97 | - |
(Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly-Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 | pH not specified in the publication, 37°C, recombinant enzyme mutant lacking the C-terminal domain | Homo sapiens | |
3.4.24.80 | 9.27 | - |
(Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly-Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 | pH not specified in the publication, 37°C, recombinant wild-type membrane-bound enzyme | Homo sapiens | |
3.4.24.80 | 45.2 | - |
(Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly-Cys(Mob)-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 | pH not specified in the publication, 37°C, recombinant soluble enzyme | Homo sapiens |