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Literature summary extracted from

  • Dikaya, V.S.; Solovyeva, A.I.; Sidorov, R.A.; Solovyev, P.A.; Stepanova, A.Y.
    The relationship between endogenous beta-glucuronidase activity and biologically active flavones-aglycone contents in hairy roots of Baikal skullcap (2018), Chem. Biodivers., 15, e1700409 .
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.167 baicalin + H2O Scutellaria baicalensis
-
 baicalein + D-glucuronate
-
 ?
3.2.1.167 additional information Scutellaria baicalensis domination of the glucuronide wogonoside is a characteristic feature of the hairy roots culture, while glucuronide baicalin is predominant in intact plant roots and also in the hairy roots. Alteration in contents of the main flavones in hairy roots culture of the Baikal skullcap during culturing, overview ?
-
 ?
3.2.1.167 wogonoside + H2O Scutellaria baicalensis
-
 wogonin + D-glucuronate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.167 Scutellaria baicalensis Q9LRC8
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.167 hairy root
-
 Scutellaria baicalensis
-
3.2.1.167 additional information relationship between contents of principal flavones in hairy roots of Scutellaria baicalensis with the activity of the beta-glucuronidase (sGUS) enzyme during a culturing cycle, overview. The highest contents of aglycones, baicalin and wogonin is observed at the growth days 8, 14, and 71 and reach 45, 41, and 62% (based on the total weight of hairy roots of the Baikal skullcap), correspondingly. Their accumulation is accompanied by increase of the sGUS activity. The enzyme activity is characterized by significant and reasonable correlation only with the wogonin contents Scutellaria baicalensis
-
3.2.1.167 root
-
 Scutellaria baicalensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.167 4-methylumbelliferyl-beta-D-glucuronide + H2O
-
 Scutellaria baicalensis 4-methylumbelliferol + D-glucuronate
-
 ?
3.2.1.167 baicalin + H2O
-
 Scutellaria baicalensis baicalein + D-glucuronate
-
 ?
3.2.1.167 additional information domination of the glucuronide wogonoside is a characteristic feature of the hairy roots culture, while glucuronide baicalin is predominant in intact plant roots and also in the hairy roots. Alteration in contents of the main flavones in hairy roots culture of the Baikal skullcap during culturing, overview Scutellaria baicalensis ?
-
 ?
3.2.1.167 wogonoside + H2O
-
 Scutellaria baicalensis wogonin + D-glucuronate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.167 baicalinase
-
 Scutellaria baicalensis
3.2.1.167 beta-glucuronidase
-
 Scutellaria baicalensis
3.2.1.167 GUS
-
 Scutellaria baicalensis
3.2.1.167 sGUS
-
 Scutellaria baicalensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.167 37
-
-
 Scutellaria baicalensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.167 4.5
-
-
 Scutellaria baicalensis

Expression

EC Number Organism Comment Expression
3.2.1.167 Scutellaria baicalensis increase in the glucuronidase activity due to mechanical stress (e.g. dissecting roots) up
3.2.1.167 Scutellaria baicalensis increase in the glucuronidase activity during the first weeks of cultivation followed by mechanical stress (e.g. dissecting roots) up

General Information

EC Number General Information Comment Organism
3.2.1.167 metabolism the central precursor metabolite of wogonin and baicalein and their glycosylated forms is chrysin Scutellaria baicalensis
3.2.1.167 physiological function relationship between contents of principal flavones in hairy roots of Scutellaria baicalensis with the activity of the beta-glucuronidase (sGUS) enzyme during a culturing cycle. The enzyme activity is characterized by significant and reasonable correlation only with the wogonin contents. The endogenous beta-glucuronidase (sGUS), that catalyzes the cleavage of the glucuronic acid residue from the glycosylated forms of phenolic compounds Scutellaria baicalensis
3.2.1.167 physiological function the beta-glucuronidase enzyme is involved in the formation of the more active aglycone flavones in the mdicinal plant. The enzyme activity is characterized by significant correlation only with the wogonin contents, whereas the correlation for the baicalin is insignificant Scutellaria baicalensis