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Literature summary extracted from

  • Mesicek, J.; Lee, H.; Feldman, T.; Jiang, X.; Skobeleva, A.; Berdyshev, E.V.; Haimovitz-Friedman, A.; Fuks, Z.; Kolesnick, R.
    Ceramide synthases 2, 5, and 6 confer distinct roles in radiation-induced apoptosis in HeLa cells (2010), Cell. Signal., 22, 1300-1307 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.24 gene CERS5, recombinant FLAG-tagged isozyme overexpression in HeLa cells, quantitative real-time PCR enzyme expression analysis, CerS5 overexpression significantly increases apoptosis in a time- and dose-dependent manner compared to empty vector-transfected controls. CerS2, CerS5, and CerS6 form heterocomplexes in transformed HeLa cells Homo sapiens
2.3.1.24 gene CERS6, recombinant FLAG-tagged isozyme overexpression in HeLa cells, quantitative real-time PCR enzyme expression analysis, CerS6 overexpression yields no significant differences in IR-induced apoptosis compared to empty vector-transfected cells. CerS2, CerS5, and CerS6 form heterocomplexes in transformed HeLa cells Homo sapiens
2.3.1.297 gene CERS2, recombinant FLAG-tagged isozyme overexpression in HeLa cells, quantitative real-time PCR enzyme expression analysis. CerS2, CerS5, and CerS6 form heterocomplexes in transformed HeLa cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.24 additional information CerS6 knockdown by shRNA Homo sapiens
2.3.1.297 additional information CerS2 knockdown by shRNA Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.24 additional information
-
 additional information nonlinear fitting and Michaelis-Menten kinetic analyses Homo sapiens
2.3.1.297 additional information
-
 additional information nonlinear fitting and Michaelis-Menten kinetic analyses Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.24 mitochondrial membrane
-
 Homo sapiens 31966
-
2.3.1.297 mitochondrial membrane
-
 Homo sapiens 31966
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.24 sphinganine + palmitoyl-CoA Homo sapiens
-
 N-palmitoylsphinganine + CoA
-
 ?
2.3.1.297 sphinganine + lignoceroyl-CoA Homo sapiens
-
 N-lignoceroylsphinganine + CoA
-
 ?
2.3.1.297 sphinganine + nervonoyl-CoA Homo sapiens
-
 N-nervonoylsphinganine + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.24 Homo sapiens Q6ZMG9
-
-
2.3.1.24 Homo sapiens Q8N5B7
-
-
2.3.1.291 Homo sapiens Q8N5B7
-
-
2.3.1.297 Homo sapiens Q96G23
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.24 subcellular fractionation of HeLa cells recombinantly expressing the CerS isozyme Homo sapiens
2.3.1.297 subcellular fractionation of HeLa cells recombinantly expressing the CerS isozyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.291 HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.24 sphinganine + palmitoyl-CoA
-
 Homo sapiens N-palmitoylsphinganine + CoA
-
 ?
2.3.1.297 sphinganine + lignoceroyl-CoA
-
 Homo sapiens N-lignoceroylsphinganine + CoA
-
 ?
2.3.1.297 sphinganine + nervonoyl-CoA
-
 Homo sapiens N-nervonoylsphinganine + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.24 ceramide synthase 5
-
 Homo sapiens
2.3.1.24 ceramide synthase 6
-
 Homo sapiens
2.3.1.24 CerS5
-
 Homo sapiens
2.3.1.24 CerS6
-
 Homo sapiens
2.3.1.291 CerS5
-
 Homo sapiens
2.3.1.297 ceramide synthase 2
-
 Homo sapiens
2.3.1.297 CerS2
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.24 7.4
-
 assay at Homo sapiens
2.3.1.297 7.4
-
 assay at Homo sapiens

Expression

EC Number Organism Comment Expression
2.3.1.24 Homo sapiens ionizing radiation (IR) induces de novo synthesis of ceramide by specifically activating CerS isoforms 2, 5, and 6 up
2.3.1.291 Homo sapiens ionizing radiation induces de novo synthesis of ceramide by specifically activating CerS isoforms 2, 5, and 6 up
2.3.1.297 Homo sapiens ionizing radiation (IR) induces de novo synthesis of ceramide by specifically activating CerS isoforms 2, 5, and 6 up

General Information

EC Number General Information Comment Organism
2.3.1.24 malfunction CerS6 knockdown might decrease apoptosis compared to normal irradiated HeLa cells Homo sapiens
2.3.1.24 malfunction overexpression of CerS5 increases apoptosis in HeLa cells. CerS2 and CerS5 overexpression significantly alters apoptosis Homo sapiens
2.3.1.24 metabolism regulation of CerS isozymes, overview. The interplay among the CerS proteins takes place in a stress stimulus-, cell type- and subcellular compartment-specific manner. CerS2 and CerS5 overexpression significantly alters apoptosis, determination of ionizing radiation-induced mitochondrial ceramide elevations via CerS2, 5, and 6, overview. CerS2 and CerS5 overexpressions defines opposing roles in radiation-induced mitochondrial apoptosis Homo sapiens
2.3.1.24 metabolism regulation of CerS isozymes, overview. The interplay among the CerS proteins takes place in a stress stimulus-, cell type- and subcellular compartment-specific manner. CerS6 overexpression yields no significant differences in IR-induced apoptosis compared to empty vector-transfected cells. Determination of ionizing radiation-induced mitochondrial ceramide elevations via CerS2, 5, and 6, overview Homo sapiens
2.3.1.24 physiological function selective tissue and subcellular distribution of the six mammalian CerS isoforms, combined with distinct fatty acyl chain length substrate preferences, implicate differential functions of specific ceramide species in cellular signaling. Ionizing radiation (IR) induces de novo synthesis of ceramide to influence HeLa cell apoptosis by specifically activating isozymes CerS isoforms 2, 5, and 6 that generate opposing anti- and pro-apoptotic ceramides in mitochondrial membranes. Isozymes CerS5 and CerS6 each confer about 50% of the C16:0 CerS baseline synthetic activity, both are required for IR-induced activity. IR-induced CerS-mediated ceramide generation, and subsequent apoptosis, occurs in a cell-type specific manner. CerS2 and CerS5 overexpressions defines opposing roles in radiation-induced mitochondrial apoptosis Homo sapiens
2.3.1.24 physiological function selective tissue and subcellular distribution of the six mammalian CerS isoforms, combined with distinct fatty acyl chain length substrate preferences, implicate differential functions of specific ceramide species in cellular signaling. Ionizing radiation (IR) induces de novo synthesis of ceramide to influence HeLa cell apoptosis by specifically activating isozymes CerS isoforms 2, 5, and 6 that generate opposing anti- and pro-apoptotic ceramides in mitochondrial membranes. Isozymes CerS5 and CerS6 each confer about 50% of the C16:0 CerS baseline synthetic activity, both are required for IR-induced activity. IR-induced CerS-mediated ceramide generation, and subsequent apoptosis, occurs in a cell-type specific manner. CerS6 overexpression yields no significant differences in IR-induced apoptosis compared to empty vector-transfected cells Homo sapiens
2.3.1.291 physiological function ionizing radiation induces de novo synthesis of ceramide to influence HeLa cell apoptosis by specifically activating CerS isoforms 2, 5, and 6 that generate opposing anti- and pro-apoptotic ceramides in mitochondrial membranes. Isoforms CerS5 and CerS6 each confer about 50% of the C16:0 CerS baseline synthetic activity, both are required for ionizing radiation-induced activity. Isoforms CerS2, 5, and 6 might exist as heterocomplexes in HeLa cells Homo sapiens
2.3.1.297 malfunction overexpression of CerS2 results in partial protection from IR-induced apoptosis. Knockdown studies determines that CerS2 is responsible for all observable IR-induced C24:0 CerS activity. CerS2 and CerS5 overexpression significantly alters apoptosis Homo sapiens
2.3.1.297 metabolism regulation of CerS isozymes, overview. The interplay among the CerS proteins takes place in a stress stimulus-, cell type-, and subcellular compartment-specific manner. CerS2 and CerS5 overexpression significantly alters apoptosis. Determination of ionizing radiation-induced mitochondrial ceramide elevations via CerS2, 5, and 6, overview. CerS2 and CerS5 overexpressions defines opposing roles in radiation-induced mitochondrial apoptosis Homo sapiens
2.3.1.297 physiological function selective tissue and subcellular distribution of the six mammalian CerS isoforms, combined with distinct fatty acyl chain length substrate preferences, implicate differential functions of specific ceramide species in cellular signaling. Ionizing radiation (IR) induces de novo synthesis of ceramide to influence HeLa cell apoptosis by specifically activating isozymes CerS isoforms 2, 5, and 6 that generate opposing anti- and pro-apoptotic ceramides in mitochondrial membranes. Isozyme CerS2 is responsible for all observable IR-induced C24:0 CerS activity. IR-induced CerS-mediated ceramide generation, and subsequent apoptosis, occurs in a cell-type specific manner. CerS2 and CerS5 overexpressions defines opposing roles in radiation-induced mitochondrial apoptosis Homo sapiens