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Literature summary extracted from

  • Sernee, M.; Ralton, J.; Nero, T.; Sobala, L.; Kloehn, J.; Vieira-Lara, M.; Cobbold, S.; Stanton, L.; Pires, D.; Hanssen, E.; Males, A.; Ward, T.; Bastidas, L.; van der Peet, P.; Parker, M.; Ascher, D.; Williams, S.; Davies, G.; McConville, M.
    A family of dual-activity glycosyltransferase-phosphorylases mediates mannogen turnover and virulence in Leishmania parasites (2019), Cell Host Microbe, 26, 385-399.e9 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.374 D145N isoform Mtp2, little effect on transferase activity Leishmania mexicana
2.4.1.374 D94N isoform Mtp2, complete loss of transferase activity Leishmania mexicana
2.4.1.374 H161R isoform Mtp2, reduced transferase activity Leishmania mexicana

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.374 Leishmania mexicana
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-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.374 GDP-alpha-D-mannose + [(1->2)-beta-D-mannosyl]8
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 Leishmania mexicana GDP + [(1->2)-beta-D-mannosyl]9
-
 r
2.4.1.374 additional information MTP1 alone produces hyper-elongated mannogen oligomers (DP >60), while expression of MTP2 results in synthesis of a restricted pool of short mannogen oligomers (DP 2-10). Co-expression of both MTP1 and 2 results in an intermediate mannogen profile containing both short and long mannogen oligomers (DP 4 to >60) Leishmania mexicana ?
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.374 mannosyltransferase/phosphorylase
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 Leishmania mexicana
2.4.1.374 MTP1
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 Leishmania mexicana
2.4.1.374 MTP2
-
 Leishmania mexicana

General Information

EC Number General Information Comment Organism
2.4.1.374 physiological function enzyme catalyzes both the sugar nucleotide-dependent biosynthesis and phosphorolytic turnover of mannogen, glycosyltransferase family 108. Isoforms MTP1 and 2 function primarily as GDPMan-dependent beta-1,2-mannosyltransferases. Parasites lacking isoforms Mtp1-7 do not induce lesions or proliferate in highly susceptible BALB/c mice. Expression of MTP1 alone or MTP1/MTP2 together in the absence of phosphorolytic Mtps exacerbates the loss of virulence phenotype, with no recovery of parasites from tissue biopsies. The mutant lacking Mtp2 does not induce lesions in BALB/c mice even when a high inoculum is used Leishmania mexicana