Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bose, R.; Verheji, M.; Haimovitz-Friedman, A.; Scotto, K.; Fuks, Z.; Kolesnick, R.
    Ceramide synthase mediates daunorubicin-induced apoptosis an alternative mechanism for generating death signals (1995), Cell, 82, 405-414 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.24 daunorubicin an anthracycline used in the treatment of leukemia, increases the enzyme activity and ceramide production, and inhibits the apoptosis of leukmeia cells Homo sapiens
2.3.1.24 daunorubicin an anthracycline used in the treatment of leukemia, increases the enzyme activity and ceramide production, and inhibits the apoptosis of leukmeia cells, 70% increase of activity in microsomes at 0.01 mM in treatment of cell cultures for 6 h Mus musculus
2.3.1.24 additional information vincristine (100 nM), methotrexate (200 nM), etoposide (0.003 mM), and cis-platinum (0.05 mM) do not stimulate ceramide synthase Mus musculus
2.3.1.24 additional information vincristine (100 nM), methotrexate (200 nM), etoposide (0.003 mM), and cis-platinum (0.05 mM) do not stimulate ceramide synthase Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.24 fumonisin B1 a natural product of the fungus Fusarium moniliforme that acts as a mycotoxin in mammalian cells Homo sapiens
2.3.1.24 fumonisin B1 a natural product of the fungus Fusarium moniliforme that acts as a mycotoxin in mammalian cells. It does not affect the rate of cell proliferation or cell viability of P338 cells during 24 h at 0.1 mM, but inhibits 80% and 60% of the apoptotic lethal effect produced by 200 nM and 300 nM daunorubicin, respectively. Increasing the dose of daunorubicin to greater than 500 nM, it also abrogates the inhibitory effect of fumonisin B1 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.24 additional information
-
 additional information kinetics in presence of daunorubicin Mus musculus
2.3.1.24 additional information
-
 additional information kinetics in presence of daunorubicin Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.24 microsome
-
 Mus musculus
-
-
2.3.1.24 microsome
-
 Homo sapiens
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.24 Homo sapiens
-
-
-
2.3.1.24 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.24 P-388 cell
-
 Mus musculus
-
2.3.1.24 U-937 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.24 sphinganine + palmitoyl-CoA
-
 Mus musculus N-palmitoyl-DL-dihydrosphingosine + CoA
-
 ?
2.3.1.24 sphinganine + palmitoyl-CoA
-
 Homo sapiens N-palmitoyl-DL-dihydrosphingosine + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.24 ceramide synthase
-
 Mus musculus
2.3.1.24 ceramide synthase
-
 Homo sapiens
2.3.1.24 sphinganine N-acyl transferase synthase
-
 Mus musculus
2.3.1.24 sphinganine N-acyl transferase synthase
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.3.1.24 metabolism the sphingomyelin pathway, which is initiated by sphingomyelin hydrolysis to generate the second messenger ceramide, signals apoptosis for tumor necrosis factor a, Fas, and ionizing radiation Mus musculus
2.3.1.24 metabolism the sphingomyelin pathway, which is initiated by sphingomyelin hydrolysis to generate the second messenger ceramide, signals apoptosis for tumor necrosis factor a, Fas, and ionizing radiation Homo sapiens
2.3.1.24 physiological function ceramide synthase mediates daunorubicin-induced apoptosis, an alternative mechanism for generating death signals. Ceramide synthesis appears obligatory for daunorubicin-induced apoptosis, since fumonisin B1, a natural specific inhibitor of ceramide synthase, blocks daunorubicin-induced ceramide elevation and apoptosis. Ceramide mimics daunorubicin in inducing apoptosis Mus musculus
2.3.1.24 physiological function ceramide synthase mediates daunorubicin-induced apoptosis, an alternative mechanism for generating death signals. Ceramide synthesis appears obligatory for daunorubicin-induced apoptosis, since fumonisin B1, a natural specific inhibitor of ceramide synthase, blocks daunorubicin-induced ceramide elevation and apoptosis. Ceramide mimics daunorubicin in inducing apoptosis Homo sapiens