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Literature summary extracted from

  • Yoshimi, Y.; Yaguchi, K.; Kaneko, S.; Tsumuraya, Y.; Kotake, T.
    Properties of two fungal endo-beta-1,3-galactanases and their synergistic action with an exo-beta-1,3-galactanase in degrading arabinogalactan-proteins (2017), Carbohydr. Res., 453-454, 26-35 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.181 DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Pichia pastoris Neurospora crassa
3.2.1.181 DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Pichia pastoris Aspergillus flavus
3.2.1.181 DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Pichia pastoris Flammulina velutipes

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.181 Aspergillus flavus
-
-
-
3.2.1.181 Flammulina velutipes
-
-
-
3.2.1.181 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.181 recombinant enzyme 1.2fold from Pichia pastoris by cation exchange chromatography and gel filtration Neurospora crassa
3.2.1.181 recombinant enzyme 1.6fold from Pichia pastoris by cation exchange chromatography and gel filtration Flammulina velutipes
3.2.1.181 recombinant enzyme 1.7fold from Pichia pastoris by cation exchange chromatography and gel filtration Aspergillus flavus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.181 1
-
 purified recombinant enzyme, pH 5.0, 40°C Aspergillus flavus
3.2.1.181 15
-
 purified recombinant enzyme, pH 5.0, 45°C Neurospora crassa
3.2.1.181 17
-
 purified recombinant enzyme, pH 5.0, 40°C Flammulina velutipes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.181 arabinogalactan + H2O from radish leaves Flammulina velutipes ?
-
 ?
3.2.1.181 arabinogalactan + H2O the enzyme specifically acts on beta-1,3-galactan within the plant proteoglycansin arabinogalactan-proteins (AGPs) in an endo-manner. The carbohydrate moieties of AGPs consist of beta-1,3-galactan main chains and beta-1,6-galactan side chains, to which other auxiliary sugars are attached. Enzyme Af3G hardly hydrolyze native arabinogalactan-proteins (AGPs) alone but acts synergistically with a fungal exo-beta-1,3-galactanase on the AGP Aspergillus flavus ?
-
 ?
3.2.1.181 arabinogalactan + H2O the enzyme specifically acts on beta-1,3-galactan within the plant proteoglycansin arabinogalactan-proteins (AGPs) in an endo-manner. The carbohydrate moieties of AGPs consist of beta-1,3-galactan main chains and beta-1,6-galactan side chains, to which other auxiliary sugars are attached. Enzyme NcEn3GAL hardly hydrolyze native arabinogalactan-proteins (AGPs) alone but acts synergistically with a fungal exo-beta-1,3-galactanase on the AGP. Very low activity on native gum arabic and the larch arabinogalactan Neurospora crassa ?
-
 ?
3.2.1.181 beta-1,3-galactan + H2O best substrate Neurospora crassa oligo-beta-(1->3)-D-galactose
-
 ?
3.2.1.181 beta-1,3-galactan + H2O best substrate Aspergillus flavus oligo-beta-(1->3)-D-galactose
-
 ?
3.2.1.181 beta-1,3-galactan + H2O best substrate Flammulina velutipes oligo-beta-(1->3)-D-galactose
-
 ?
3.2.1.181 beta-1,3-galactotetraose + H2O
-
 Flammulina velutipes 2 beta-1,3-galactobiose
-
 ?
3.2.1.181 beta-1,3-galactotetraose + H2O the enzyme seems to prefer hydrolyzing the central beta-1,3-glycosidic linkage of three in beta-1,3-galactotetraose Neurospora crassa 2 beta-1,3-galactobiose
-
 ?
3.2.1.181 beta-1,3-galactotetraose + H2O the enzyme seems to prefer hydrolyzing the central beta-1,3-glycosidic linkage of three in beta-1,3-galactotetraose Aspergillus flavus 2 beta-1,3-galactobiose
-
 ?
3.2.1.181 beta-1,4-galactan + H2O from lupin Neurospora crassa ?
-
 ?
3.2.1.181 beta-1,4-galactan + H2O from lupin Aspergillus flavus ?
-
 ?
3.2.1.181 beta-1,4-galactan + H2O from lupin Flammulina velutipes ?
-
 ?
3.2.1.181 gum arabic beta-1,3-galactan I + H2O
-
 Neurospora crassa ?
-
 ?
3.2.1.181 gum arabic beta-1,3-galactan I + H2O
-
 Aspergillus flavus ?
-
 ?
3.2.1.181 gum arabic beta-1,3-galactan I + H2O
-
 Flammulina velutipes ?
-
 ?
3.2.1.181 gum arabic beta-1,3-galactan II + H2O
-
 Neurospora crassa ?
-
 ?
3.2.1.181 gum arabic beta-1,3-galactan II + H2O
-
 Aspergillus flavus ?
-
 ?
3.2.1.181 gum arabic beta-1,3-galactan II + H2O
-
 Flammulina velutipes ?
-
 ?
3.2.1.181 larch arabinogalactan + H2O
-
 Neurospora crassa ?
-
 ?
3.2.1.181 larch arabinogalactan + H2O
-
 Aspergillus flavus ?
-
 ?
3.2.1.181 larch arabinogalactan + H2O
-
 Flammulina velutipes ?
-
 ?
3.2.1.181 larch beta-galactan I + H2O
-
 Neurospora crassa ?
-
 ?
3.2.1.181 larch beta-galactan I + H2O
-
 Aspergillus flavus ?
-
 ?
3.2.1.181 larch beta-galactan I + H2O
-
 Flammulina velutipes ?
-
 ?
3.2.1.181 larch beta-galactan II + H2O
-
 Neurospora crassa ?
-
 ?
3.2.1.181 larch beta-galactan II + H2O
-
 Aspergillus flavus ?
-
 ?
3.2.1.181 larch beta-galactan II + H2O
-
 Flammulina velutipes ?
-
 ?
3.2.1.181 larch beta-galactan III + H2O
-
 Neurospora crassa ?
-
 ?
3.2.1.181 larch beta-galactan III + H2O
-
 Aspergillus flavus ?
-
 ?
3.2.1.181 larch beta-galactan III + H2O
-
 Flammulina velutipes ?
-
 ?
3.2.1.181 additional information substrate specificity, overview. Gum arabic from acacia tree is a poor substrate Flammulina velutipes ?
-
 ?
3.2.1.181 additional information very low activity on native gum arabic and the larch arabinogalactan. Substrate specificity, overview Neurospora crassa ?
-
 ?
3.2.1.181 additional information very low activity on native gum arabic and the larch arabinogalactan. Substrate specificity, overview Aspergillus flavus ?
-
 ?
3.2.1.181 radish leaf arabinogalactan + H2O
-
 Neurospora crassa ?
-
 ?
3.2.1.181 radish leaf arabinogalactan + H2O
-
 Aspergillus flavus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.181 ? x * 42000, recombinant enzyme, SDS-PAGE Neurospora crassa
3.2.1.181 ? x * 37000, recombinant enzyme, SDS-PAGE Aspergillus flavus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.181 Af3G
-
 Aspergillus flavus
3.2.1.181 endo-beta-1,3-galactanase
-
 Neurospora crassa
3.2.1.181 endo-beta-1,3-galactanase
-
 Aspergillus flavus
3.2.1.181 endo-beta-1,3-galactanase
-
 Flammulina velutipes
3.2.1.181 FvEn3GAL
-
 Flammulina velutipes
3.2.1.181 NcEn3GAL
-
 Neurospora crassa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.181 40
-
 recombinant enzyme Aspergillus flavus
3.2.1.181 45
-
 assay at Flammulina velutipes
3.2.1.181 45
-
 recombinant enzyme Neurospora crassa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.181 10 60 activity range, profile overview, recombinant enzyme Neurospora crassa
3.2.1.181 10 60 40% of maximal activity at 50°C and 50% at 10°C, profile overview, recombinant enzyme Aspergillus flavus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.181 5
-
 recombinant enzyme Neurospora crassa
3.2.1.181 5
-
 recombinant enzyme Aspergillus flavus
3.2.1.181 5
-
 assay at Flammulina velutipes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.181 3 7 activity range, profile overview, recombinant enzyme Aspergillus flavus
3.2.1.181 3 7 over 50% of maximal activity within this range, inactive above pH 8.0, profile overview, recombinant enzyme Neurospora crassa

General Information

EC Number General Information Comment Organism
3.2.1.181 evolution the enzyme belongs to the glycosyl hydrolase family 16, GH16 Neurospora crassa
3.2.1.181 evolution the enzyme belongs to the glycosyl hydrolase family 16, GH16 Aspergillus flavus
3.2.1.181 evolution the enzyme belongs to the glycosyl hydrolase family 16, GH16 Flammulina velutipes
3.2.1.181 physiological function the endo-beta-1,3-galactanases presumably aid hydrolysis of arabinogalactan-proteins (AGPs) by internally breaking up AGPs, which creates more sites of attack for exo-beta-1,3-galactanase Neurospora crassa
3.2.1.181 physiological function the endo-beta-1,3-galactanases presumably aid hydrolysis of arabinogalactan-proteins (AGPs) by internally breaking up AGPs, which creates more sites of attack for exo-beta-1,3-galactanase Aspergillus flavus
3.2.1.181 physiological function the endo-beta-1,3-galactanases presumably aid hydrolysis of arabinogalactan-proteins (AGPs) by internally breaking up AGPs, which creates more sites of attack for exo-beta-1,3-galactanase Flammulina velutipes