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Literature summary extracted from
- Novel substrate specificities of two lacto-N-biosidases towards beta-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1 (2015), Carbohydr. Res., 408, 18-24 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.140 | recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Bifidobacterium bifidum |
| 3.2.1.140 | recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Bifidobacterium longum subsp. Longum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.140 | additional information | no inhibition by Gal nor GlcNAc | Bifidobacterium bifidum | |
| 3.2.1.140 | additional information | no inhibition by Gal nor GlcNAc | Bifidobacterium longum subsp. Longum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.140 | 0.005 | - |
4-nitrophenyl-Gal-beta-(1->3)-GalNAc | pH 6.0, 30°C | Bifidobacterium longum subsp. Longum |  |
| 3.2.1.140 | 0.0088 | - |
Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc | pH 6.0, 30°C | Bifidobacterium bifidum | |
| 3.2.1.140 | 0.12 | - |
4-nitrophenyl-beta-lacto-N-bioside | pH 6.0, 30°C | Bifidobacterium longum subsp. Longum | |
| 3.2.1.140 | 0.4 | - |
Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc | pH 6.0, 25°C | Bifidobacterium longum subsp. Longum | |
| 3.2.1.140 | 2.5 | - |
Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc | pH 6.0, 30°C | Bifidobacterium longum subsp. Longum | |
| 3.2.1.140 | 15 | - |
Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc | pH 6.0, 25°C | Bifidobacterium longum subsp. Longum | |
| 3.2.1.140 | 67 | - |
Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc | pH 6.0, 25°C | Bifidobacterium bifidum | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.140 | lacto-N-tetraose + H2O | Bifidobacterium bifidum | - |
lacto-N-biose + lactose | - |
? | |
| 3.2.1.140 | lacto-N-tetraose + H2O | Bifidobacterium longum subsp. Longum | - |
lacto-N-biose I + lactose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.140 | Bifidobacterium bifidum | B3TLD6 | - |
- |
| 3.2.1.140 | Bifidobacterium longum subsp. Longum | A0A024QYS6 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.140 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) | Bifidobacterium bifidum |
| 3.2.1.140 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) | Bifidobacterium longum subsp. Longum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.140 | 4-nitrophenyl-beta-lacto-N-bioside + H2O | - |
Bifidobacterium bifidum | 4-nitrophenol + lacto-N-biose | - |
? | |
| 3.2.1.140 | 4-nitrophenyl-beta-lacto-N-bioside + H2O | - |
Bifidobacterium longum subsp. Longum | 4-nitrophenol + lacto-N-biose | - |
? | |
| 3.2.1.140 | 4-nitrophenyl-Gal-beta-(1->3)-GalNAc + H2O | - |
Bifidobacterium bifidum | 4-nitrophenol + Gal-beta-(1->3)-GalNAc | - |
? | |
| 3.2.1.140 | 4-nitrophenyl-Gal-beta-(1->3)-GalNAc + H2O | - |
Bifidobacterium longum subsp. Longum | 4-nitrophenol + Gal-beta-(1->3)-GalNAc | - |
? | |
| 3.2.1.140 | Fuc-alpha-(1->2)-Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc + H2O | i.e. Globo H | Bifidobacterium longum subsp. Longum | Fuc-alpha-(1->2)-Gal-beta-(1->3)-GalNAc + Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc | - |
? | |
| 3.2.1.140 | Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc + H2O | i.e. LNFP I | Bifidobacterium longum subsp. Longum | Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc + Gal-beta-(1->4)-Glc | - |
? | |
| 3.2.1.140 | Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc + H2O | i.e. Gb5 | Bifidobacterium bifidum | Gal-beta-(1->3)-GalNAc + Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc | - |
? | |
| 3.2.1.140 | Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc + H2O | i.e. Gb5 | Bifidobacterium longum subsp. Longum | Gal-beta-(1->3)-GalNAc + Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc | - |
? | |
| 3.2.1.140 | Gal-beta-(1->3)-GalNAc-beta-(1->4)-Gal-beta-(1->4)-Glc + H2O | i.e. GA1 | Bifidobacterium bifidum | Gal-beta-(1->3)-GlcNAc + Gal-beta-(1->4)-Glc | - |
? | |
| 3.2.1.140 | Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc + H2O | i.e. LNT | Bifidobacterium bifidum | Gal-beta-(1->3)-GlcNAc + Gal-beta-(1->4)-Glc | - |
? | |
| 3.2.1.140 | Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc + H2O | i.e. LNT | Bifidobacterium longum subsp. Longum | Gal-beta-(1->3)-GlcNAc + Gal-beta-(1->4)-Glc | - |
? | |
| 3.2.1.140 | lacto-N-tetraose + H2O | - |
Bifidobacterium bifidum | lacto-N-biose + lactose | - |
? | |
| 3.2.1.140 | lacto-N-tetraose + H2O | - |
Bifidobacterium longum subsp. Longum | lacto-N-biose I + lactose | - |
? | |
| 3.2.1.140 | additional information | LnbB, a member of the glycoside hydrolase family 20 isolated from Bifidobacterium bifidum, releases Galbeta1-3GalNAc (GNB) from Gb5 and GA1 oligosaccharides. Substrate specificity, overview. Glycan-scavenging activity of LnbB, i.e. the activity of the enzyme towards the oligosaccharides of globo- and ganglio-series sphingolipids. Activity of GH20 LnbB towards Gb5 oligosaccharide is significantly low. No activity with Fuc-alpha-(1->2)-Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc (Globo H) and Fuc-alpha-(1->2)Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc (LNFP I) | Bifidobacterium bifidum | ? | - |
? | |
| 3.2.1.140 | additional information | LnbX, a non-classified member of the glycoside hydrolase family, isolated from Bifidobacterium longum subsp. longum, liberates galacto-N-biose (GNB: Gal-beta-(1->3)-GalNAc) and 2'-fucosyl GNB (a type-4 trisaccharide) from Gb5 pentasaccharide and globo H hexasaccharide, respectively. Substrate specificity, overview. Glycan-scavenging activity of LnbX, i.e. the activity of the enzyme towards the oligosaccharides of globo- and ganglio-series sphingolipids. No activity with Gal-beta-(1->3)-GalNAc-beta-(1->4)-Gal-beta-(1->4)-Glc | Bifidobacterium longum subsp. Longum | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.140 | LnbB | - |
Bifidobacterium bifidum |
| 3.2.1.140 | LnbX | - |
Bifidobacterium longum subsp. Longum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.140 | 30 | - |
assay at | Bifidobacterium bifidum |
| 3.2.1.140 | 30 | - |
assay at | Bifidobacterium longum subsp. Longum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.140 | 0.29 | - |
Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc | pH 6.0, 30°C | Bifidobacterium bifidum | |
| 3.2.1.140 | 0.84 | - |
Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc | pH 6.0, 30°C | Bifidobacterium longum subsp. Longum | |
| 3.2.1.140 | 1.5 | - |
4-nitrophenyl-Gal-beta-(1->3)-GalNAc | pH 6.0, 30°C | Bifidobacterium longum subsp. Longum | |
| 3.2.1.140 | 4.9 | - |
4-nitrophenyl-Gal-beta-(1->3)-GalNAc | pH 6.0, 30°C | Bifidobacterium bifidum | |
| 3.2.1.140 | 14 | - |
Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc | pH 6.0, 25°C | Bifidobacterium longum subsp. Longum | |
| 3.2.1.140 | 16 | - |
4-nitrophenyl-beta-lacto-N-bioside | pH 6.0, 30°C | Bifidobacterium bifidum | |
| 3.2.1.140 | 42 | - |
Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc | pH 6.0, 25°C | Bifidobacterium bifidum | |
| 3.2.1.140 | 88 | - |
4-nitrophenyl-beta-lacto-N-bioside | pH 6.0, 30°C | Bifidobacterium longum subsp. Longum | |
| 3.2.1.140 | 110 | - |
Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc | pH 6.0, 25°C | Bifidobacterium longum subsp. Longum | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.140 | 6 | - |
assay at | Bifidobacterium bifidum |
| 3.2.1.140 | 6 | - |
assay at | Bifidobacterium longum subsp. Longum |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.2.1.140 | evolution | LnbB is a member of the glycoside hydrolase family 20, GH20 | Bifidobacterium bifidum |
| 3.2.1.140 | evolution | LnbX is a non-classified member of the glycoside hydrolase family | Bifidobacterium longum subsp. Longum |
| 3.2.1.140 | physiological function | lacto-N-biosidase is a key enzyme that degrades lacto-N-tetraose, a main component of human milk oligosaccharides (HMOs, the third-most abundant solid component in human milk), into lacto-N-biose I and lactose. . Enzymatic release of beta-linked GNB from natural substrates, these unique activities may play a role in modulating the microbial composition in the gut ecosystem | Bifidobacterium bifidum |
| 3.2.1.140 | physiological function | lacto-N-biosidase is a key enzyme that degrades lacto-N-tetraose, a main component of human milk oligosaccharides (HMOs, the third-most abundant solid component in human milk), into lacto-N-biose I and lactose. Enzymatic release of beta-linked Galbeta1-3GalNAc from natural substrates, these unique activities may play a role in modulating the microbial composition in the gut ecosystem | Bifidobacterium longum subsp. Longum |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.140 | 0.336 | - |
Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc | pH 6.0, 30°C | Bifidobacterium longum subsp. Longum | |
| 3.2.1.140 | 0.63 | - |
Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc | pH 6.0, 25°C | Bifidobacterium bifidum | |
| 3.2.1.140 | 0.93 | - |
Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc | pH 6.0, 25°C | Bifidobacterium longum subsp. Longum | |
| 3.2.1.140 | 39.96 | - |
Gal-beta-(1->3)-GalNAc-beta-(1->3)-Gal-alpha-(1->4)-Gal-beta-(1->4)-Glc | pH 6.0, 30°C | Bifidobacterium bifidum | |
| 3.2.1.140 | 275 | - |
Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc | pH 6.0, 25°C | Bifidobacterium longum subsp. Longum | |
| 3.2.1.140 | 300 | - |
4-nitrophenyl-Gal-beta-(1->3)-GalNAc | pH 6.0, 30°C | Bifidobacterium longum subsp. Longum | |
| 3.2.1.140 | 733.3 | - |
4-nitrophenyl-beta-lacto-N-bioside | pH 6.0, 30°C | Bifidobacterium longum subsp. Longum | |
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