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Literature summary extracted from

  • Hossbach, J.; Busswinkel, F.; Kranz, A.; Wattjes, J.; Cord-Landwehr, S.; Moerschbacher, B.M.
    A chitin deacetylase of Podospora anserina has two functional chitin binding domains and a unique mode of action (2018), Carbohydr. Polym., 183, 1-10 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.41 phylogenetic tree, recombinant expression of Strep-tagged enzyme in Hansenula polymorpha strain RB11 Podospora anserina

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.41 additional information generation of enzyme deletion mutants lacking one or both chitin-binding domains, CBDs Podospora anserina

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.41 Co2+ 80.9% inhibition at 10 mM, 19.4% at 1 mM Podospora anserina
3.5.1.41 EDTA moderate inhibition at 10 mM Podospora anserina
3.5.1.41 Fe2+ 70.5% inhibition at 10 mM Podospora anserina
3.5.1.41 Mn2+ 9% activating at 1 mM, 22.6% inhibition at 10 mM Podospora anserina
3.5.1.41 additional information no inhibition by 100 mM acetate Podospora anserina
3.5.1.41 Zn2+ 98.3% inhibition at 10 mM, 68.3% at 1 mM Podospora anserina

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.41 Mn2+ 9% activating at 1 mM, 22.6% inhibition at 10 mM Podospora anserina

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.41 Podospora anserina B2AAQ0 i.e. Pleurage anserina
-
3.5.1.41 Podospora anserina ATCC MYA-4624 B2AAQ0 i.e. Pleurage anserina
-
3.5.1.41 Podospora anserina DSM 980 B2AAQ0 i.e. Pleurage anserina
-
3.5.1.41 Podospora anserina FGSC 10383 B2AAQ0 i.e. Pleurage anserina
-
3.5.1.41 Podospora anserina S B2AAQ0 i.e. Pleurage anserina
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.41 recombinant Strep-tagged enzyme from Hansenula polymorpha strain RB11 by StrepTactin affinity chromatography Podospora anserina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.41 acetylated chitin oligomer + H2O
-
 Podospora anserina ?
-
 ?
3.5.1.41 acetylated chitin oligomer + H2O
-
 Podospora anserina ATCC MYA-4624 ?
-
 ?
3.5.1.41 acetylated chitin oligomer + H2O
-
 Podospora anserina DSM 980 ?
-
 ?
3.5.1.41 acetylated chitin oligomer + H2O
-
 Podospora anserina FGSC 10383 ?
-
 ?
3.5.1.41 acetylated chitin oligomer + H2O
-
 Podospora anserina S ?
-
 ?
3.5.1.41 acetylated chitosan oligomer + H2O increasing activity with increasing degree of acetylation Podospora anserina ?
-
 ?
3.5.1.41 alpha-chitin + H2O
-
 Podospora anserina ?
-
 ?
3.5.1.41 alpha-chitin + H2O
-
 Podospora anserina ATCC MYA-4624 ?
-
 ?
3.5.1.41 alpha-chitin + H2O
-
 Podospora anserina DSM 980 ?
-
 ?
3.5.1.41 alpha-chitin + H2O
-
 Podospora anserina FGSC 10383 ?
-
 ?
3.5.1.41 alpha-chitin + H2O
-
 Podospora anserina S ?
-
 ?
3.5.1.41 beta-chitin + H2O
-
 Podospora anserina ?
-
 ?
3.5.1.41 beta-chitin + H2O
-
 Podospora anserina ATCC MYA-4624 ?
-
 ?
3.5.1.41 beta-chitin + H2O
-
 Podospora anserina DSM 980 ?
-
 ?
3.5.1.41 beta-chitin + H2O
-
 Podospora anserina FGSC 10383 ?
-
 ?
3.5.1.41 beta-chitin + H2O
-
 Podospora anserina S ?
-
 ?
3.5.1.41 chitin pentamer + H2O
-
 Podospora anserina ?
-
 ?
3.5.1.41 colloidal chitin + H2O high activity Podospora anserina ?
-
 ?
3.5.1.41 colloidal chitin + H2O high activity Podospora anserina ATCC MYA-4624 ?
-
 ?
3.5.1.41 colloidal chitin + H2O high activity Podospora anserina DSM 980 ?
-
 ?
3.5.1.41 colloidal chitin + H2O high activity Podospora anserina FGSC 10383 ?
-
 ?
3.5.1.41 colloidal chitin + H2O high activity Podospora anserina S ?
-
 ?
3.5.1.41 additional information analysis of the enzyme's mode of action on chitin oligomers by quantitative mass-spectrometric sequencing Podospora anserina ?
-
 ?
3.5.1.41 additional information analysis of the enzyme's mode of action on chitin oligomers by quantitative mass-spectrometric sequencing Podospora anserina ATCC MYA-4624 ?
-
 ?
3.5.1.41 additional information analysis of the enzyme's mode of action on chitin oligomers by quantitative mass-spectrometric sequencing Podospora anserina DSM 980 ?
-
 ?
3.5.1.41 additional information analysis of the enzyme's mode of action on chitin oligomers by quantitative mass-spectrometric sequencing Podospora anserina FGSC 10383 ?
-
 ?
3.5.1.41 additional information analysis of the enzyme's mode of action on chitin oligomers by quantitative mass-spectrometric sequencing Podospora anserina S ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.41 ? x * 40000, about, sequence calculation, x * 35500, recombinant enzyme, SDS-PAGE Podospora anserina

Synonyms

EC Number Synonyms Comment Organism
3.5.1.41 CDA
-
 Podospora anserina
3.5.1.41 PaCDA
-
 Podospora anserina
3.5.1.41 PODANS_1_4780
-
 Podospora anserina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.41 55
-
 broad optimum at 40-60°C Podospora anserina

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.41 10 90 activity range, profile overview Podospora anserina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.41 80
-
 purified recombinant enzyme, half-life is 11.8 min Podospora anserina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.41 10.5
-
-
 Podospora anserina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.41 4 12 activity range, almost linear increase of activity over the pH range 4.0-10.5 Podospora anserina

General Information

EC Number General Information Comment Organism
3.5.1.41 evolution the CDA from Podospora anserina (PaCDA) is closely related to Colletotrichum lindemuthianum CDA in the catalytic domain, but unique in having two chitin-binding domains. The catalytic domain of PaCDA is also functionally similar to Colletotrichum lindemuthianum CDA, though differing in detail Podospora anserina
3.5.1.41 additional information mode of action on chitin, overview Podospora anserina
3.5.1.41 physiological function proposed function of the enzyme in conversion of the insoluble substrate colloidal chitin Podospora anserina