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Literature summary extracted from

  • Wu, X.; Grover, N.; Paskaleva, E.E.; Mundra, R.V.; Page, M.A.; Kane, R.S.; Dordick, J.S.
    Characterization of the activity of the spore cortex lytic enzyme CwlJ1 (2015), Biotechnol. Bioeng., 112, 1365-1375 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.28 gene cwlJ1, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3) pLysS Bacillus anthracis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.28 additional information no inhibition by EDTA Bacillus anthracis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.28 additional information unlike most amidases, CwlJ1 does not appear to contain a divalent cation, as it retains its activity in presence of EDTA Bacillus anthracis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.28 additional information Bacillus anthracis recombinant enzyme CwlJ1 shows activity against the spore cortical fragments of Bacillus anthracis when added exogenously. CwlJ1 is active on both decoated spores and spore cortical fragments. The Bacillus spore cortex is composed of a thick layer of peptidoglycan (PG) containing alternating beta-1,4 linked N-acetylglucosamine (NAG), N-acetylmuramic acid (NAM), and muramic acid-delta-lactam (MAL) residues, with NAM residues attached to either tetrapeptide (TP) or a single L-Ala residue ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.28 Bacillus anthracis A0A1J9W3F9
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-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.28 recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography Bacillus anthracis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.28 endospore
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 Bacillus anthracis
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.28 additional information recombinant enzyme CwlJ1 shows activity against the spore cortical fragments of Bacillus anthracis when added exogenously. CwlJ1 is active on both decoated spores and spore cortical fragments. The Bacillus spore cortex is composed of a thick layer of peptidoglycan (PG) containing alternating beta-1,4 linked N-acetylglucosamine (NAG), N-acetylmuramic acid (NAM), and muramic acid-delta-lactam (MAL) residues, with NAM residues attached to either tetrapeptide (TP) or a single L-Ala residue Bacillus anthracis ?
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 ?
3.5.1.28 additional information CwlJ1 mainly recognizes large segments of glycan chains in the cortex instead of the minimal structural unit tetrasaccharide, with specificity for muramic acid-delta-lactam-containing glycan chains and preference for the tetrapeptide side chain. The enzyme cleaves Bacillus anthracis spore cortex peptidoglycan, structure, and specificity of CwlJ1 on spore core fragements, overview. Mass spectrometry analysis of muropeptides released from digested spore cortical fragments Bacillus anthracis ?
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 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.28 Cell wall hydrolase
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 Bacillus anthracis
3.5.1.28 CwlJ1
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 Bacillus anthracis
3.5.1.28 spore cortex lytic enzyme
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 Bacillus anthracis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.28 37
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 assay at Bacillus anthracis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.28 50
-
 purified recombinant His-tagged enzyme, 100 min, over 60% activity remaining Bacillus anthracis
3.5.1.28 60
-
 purified recombinant His-tagged enzyme, 75 min, over 60% activity remaining Bacillus anthracis
3.5.1.28 70
-
 purified recombinant His-tagged enzyme, 50 min, 50% activity remaining Bacillus anthracis
3.5.1.28 95
-
 purified recombinant His-tagged enzyme, loss of about 95% activity Bacillus anthracis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.28 8
-
 assay at Bacillus anthracis

General Information

EC Number General Information Comment Organism
3.5.1.28 physiological function the germination enzyme CwlJ1 plays an important role in degrading the cortex during the germination of Bacillus anthracis spores. CwlJ1 is active on both decoated spores and spore cortical fragments. Once the cortex is degraded, Ca-DPA in the spore core is released and the core becomes hydrated, leading to spore germination. The cortex contains mainly glycans, while the coat is composed of highly crosslinked proteins Bacillus anthracis