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Literature summary extracted from

  • Ivancic, V.A.; Krasinski, C.A.; Zheng, Q.; Meservier, R.J.; Spratt, D.E.; Lazo, N.D.
    Enzyme kinetics from circular dichroism of insulin reveals mechanistic insights into the regulation of insulin-degrading enzyme (2018), Biosci. Rep., 38, BSR20181416 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.56 wild-type and E111Q glutathione S-transferase tagged human insulin-degrading enzyme are expressed in Escherichia coli BL21 (DE3) codon plus competent cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.56 E111Q inactive enzyme form Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.56 0.02
-
 Insulin pH 7.4, 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.56 Zn2+ zinc metalloprotease Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.56 additional information Homo sapiens the enzyme selectively degrades biologically important substrates associated with type 2 diabetes and Alzheimer's disease ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.56 Homo sapiens P14735
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.56 insulin + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.56 additional information the enzyme selectively degrades biologically important substrates associated with type 2 diabetes and Alzheimer's disease Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.56 IDE
-
 Homo sapiens
3.4.24.56 Insulin-degrading enzyme
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.56 0.048
-
 Insulin pH 7.4, 37°C Homo sapiens

General Information

EC Number General Information Comment Organism
3.4.24.56 metabolism ATP regulates insulin-degrading enzyme-dependent degradation of insulin, but the addition of Mg2+ abolishes the regulation Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.24.56 2.4
-
 Insulin pH 7.4, 37°C Homo sapiens