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Literature summary extracted from

  • Maeda, H.; Katase, T.; Sakai, D.; Takeuchi, M.; Kusumoto, K.; Amano, H.; Ishida, H.; Abe, K.; Yamagata, Y.
    A novel non-thermostable deuterolysin from Aspergillus oryzae (2016), Biosci. Biotechnol. Biochem., 80, 1813-1819 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.39 overexpressing in Aspergillus oryzae niaD300 Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.39 Aspergillus oryzae P46076
-
-
3.4.24.39 Aspergillus oryzae Q2UP30
-
-
3.4.24.39 Aspergillus oryzae ATCC 42149 P46076
-
-
3.4.24.39 Aspergillus oryzae ATCC 42149 Q2UP30
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.39
-
 Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.39 alpha-casein + H2O degradation with low specific activity Aspergillus oryzae ?
-
 ?
3.4.24.39 benzyloxycarbonyl-Val-Lys-Met-7-amido-4-methylcoumarin + H2O second-best substrate Aspergillus oryzae ?
-
 ?
3.4.24.39 beta-lactoglobulin + H2O degradation with low specific activity Aspergillus oryzae ?
-
 ?
3.4.24.39 Boc-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O most favorable substrate Aspergillus oryzae ?
-
 ?
3.4.24.39 Boc-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
-
 Aspergillus oryzae ?
-
 ?
3.4.24.39 Boc-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O degradation with low specific activity Aspergillus oryzae ?
-
 ?
3.4.24.39 casein + H2O degradation with low specific activity Aspergillus oryzae ?
-
 ?
3.4.24.39 casein + H2O degradation with low specific activity Aspergillus oryzae ATCC 42149 ?
-
 ?
3.4.24.39 clupeine + H2O degradation with high specific activity Aspergillus oryzae ?
-
 ?
3.4.24.39 Elastin + H2O
-
 Aspergillus oryzae ?
-
 ?
3.4.24.39 Elastin + H2O
-
 Aspergillus oryzae ATCC 42149 ?
-
 ?
3.4.24.39 Fibrin + H2O degradation with low specific activity Aspergillus oryzae ?
-
 ?
3.4.24.39 Fibrin + H2O degradation with low specific activity Aspergillus oryzae ATCC 42149 ?
-
 ?
3.4.24.39 Glt-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
-
 Aspergillus oryzae ?
-
 ?
3.4.24.39 Glt-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O degradation with low specific activity Aspergillus oryzae ?
-
 ?
3.4.24.39 histone + H2O degradation with high specific activity Aspergillus oryzae ?
-
 ?
3.4.24.39 histone + H2O degradation with high specific activity Aspergillus oryzae ATCC 42149 ?
-
 ?
3.4.24.39 additional information DeuA can not digest elastin and collagen. No hydrolysis of benzyloxycarbonyl-Val-Lys-Met-7-amido-4-methylcoumarin, benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin, benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin and Suc-Gly-Pro-7-amido-4-methylcoumarin. DeuA and DeuB have similar digestion property against the protein substrates, but DeuB has a wider substrate specificity compared with DeuA Aspergillus oryzae ?
-
 ?
3.4.24.39 additional information DeuB can not digest collagen. DeuA and DeuB have similar digestion property against the protein substrates, but DeuB has a wider substrate specificity compared with DeuA Aspergillus oryzae ?
-
 ?
3.4.24.39 additional information DeuA can not digest elastin and collagen. No hydrolysis of benzyloxycarbonyl-Val-Lys-Met-7-amido-4-methylcoumarin, benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin, benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin and Suc-Gly-Pro-7-amido-4-methylcoumarin. DeuA and DeuB have similar digestion property against the protein substrates, but DeuB has a wider substrate specificity compared with DeuA Aspergillus oryzae ATCC 42149 ?
-
 ?
3.4.24.39 additional information DeuB can not digest collagen. DeuA and DeuB have similar digestion property against the protein substrates, but DeuB has a wider substrate specificity compared with DeuA Aspergillus oryzae ATCC 42149 ?
-
 ?
3.4.24.39 ovalbumin + H2O degradation with low specific activity Aspergillus oryzae ?
-
 ?
3.4.24.39 ovalbumin + H2O degradation with low specific activity Aspergillus oryzae ATCC 42149 ?
-
 ?
3.4.24.39 salmine + H2O degradation with high specific activity Aspergillus oryzae ?
-
 ?
3.4.24.39 Suc-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
-
 Aspergillus oryzae ?
-
 ?
3.4.24.39 Suc-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O degradation with low specific activity Aspergillus oryzae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.39 DeuA
-
 Aspergillus oryzae
3.4.24.39 deuB
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.39 50 60
-
 Aspergillus oryzae
3.4.24.39 50 70
-
 Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.39 30
-
 pH 7.0, 10 min, 15% loss of activity Aspergillus oryzae
3.4.24.39 30
-
 pH 7.0, 10 min, no loss of activity Aspergillus oryzae
3.4.24.39 40
-
 pH 7.0, 10 min, 10% loss of activity Aspergillus oryzae
3.4.24.39 40
-
 pH 7.0, 10 min, no loss of activity Aspergillus oryzae
3.4.24.39 50
-
 pH 7.0, 10 min, 50% loss of activity Aspergillus oryzae
3.4.24.39 50
-
 pH 7.0, 10 min, 5% loss of activity Aspergillus oryzae
3.4.24.39 60
-
 pH 7.0, 10 min, 50% loss of activity Aspergillus oryzae
3.4.24.39 60
-
 pH 7.0, 10 min, 10% loss of activity Aspergillus oryzae
3.4.24.39 70
-
 pH 7.0, 10 min, 85% loss of activity Aspergillus oryzae
3.4.24.39 70
-
 pH 7.0, 10 min, 30% loss of activity Aspergillus oryzae
3.4.24.39 80
-
 pH 7.0, 10 min, 35% loss of activity Aspergillus oryzae
3.4.24.39 80
-
 pH 7.0, 10 min, DeuB completely loses its activity Aspergillus oryzae
3.4.24.39 90
-
 pH 7.0, 10 min, 30% loss of activity Aspergillus oryzae
3.4.24.39 100
-
 pH 7.0, 10 min, 15% loss of activity Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.39 6 10
-
 Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.39 3 11 stable Aspergillus oryzae

Expression

EC Number Organism Comment Expression
3.4.24.39 Aspergillus oryzae transcription of deuB is observed at an early stage of CDN liquid culture (24 h) and the prolonged cultivation reduces the transcription of deuB. Addition of NaNO3 inhibits the upregulation of deuB transcription down
3.4.24.39 Aspergillus oryzae proteinous nitrogen sources such as skimmed milk and gelatin instead of NaNO3 upregulate the transcription level of deuB. Addition of NaNO3 inhibits the upregulation of deuB transcription up