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Literature summary extracted from

  • Kameda, K.; Suzuki, L.K.; Imai, Y.
    Further purification, characterization and salt activation of acyl-CoA synthetase from Escherichia coli (1985), Biochim. Biophys. Acta, 840, 29-36.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.2.1.3 100 mM potassium phosphate stabilizes at 4°C Escherichia coli
6.2.1.3 ATP, stabilizes at 4EC Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.3 salts at higher concentrations the chaotropic salts inhibit the enzyme activity, activity can be recovered by dilution. At low concentrations all salts, including the chaotropic salts and thelyotropic salts are equally effective in activation Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.3 salts at low concentrations all salts, including the chaotropic salts, e.g. NaNO3, NaClO4 and KSCN, and thelyotropic salts, e.g. (NH4)2SO4 and potassium phosphate are equally effective in activation. At higher concentrations the chaotropic salts inhibit the enzyme activity. Activity can be recovered by dilution Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.3 45000 47000 gel filtration Escherichia coli
6.2.1.3 50000
-
 sedimentation equilibrium ultracentrifugation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.3
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.3 1.103
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
6.2.1.3 4°C, 10 mM potassium phosphate, 12 h, 70% loss of activity. 100 mM potassium phosphate or ATP stabilizes Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.3 ATP + long-chain carboxylic acid + CoA trans-DELTA9-18:1 fatty acid Escherichia coli AMP + diphosphate + long-chain acyl-CoA
-
 ?
6.2.1.3 ATP + long-chain carboxylic acid + CoA trans-DELTA9-16:1 fatty acid Escherichia coli AMP + diphosphate + long-chain acyl-CoA
-
 ?