Literature summary extracted from

Boksha, I.S.; Lavrova, N.V.; Grishin, A.V.; Demidenko, A.V.; Lyashchuk, A.M.; Galushkina, Z.M.; Ovchinnikov, R.S.; Umyarov, A.M.; Avetisian, L.R.; Chernukha, M.I.u.; Shaginian, I.A.; Lunin, V.G.; Karyagina, A.S.
Staphylococcus simulans recombinant lysostaphin production, purification, and determination of antistaphylococcal activity (2016), Biochemistry, 81, 502-510 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.4.24.75	expressed in Escherichia coli BL21 cells	Staphylococcus simulans

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3.4.24.75	peptidoglycan + H2O	Staphylococcus simulans	peptidoglycans from cell walls of Staphylococcus aureus and Staphylococcus haemolyticus	fragments of peptidogylcan	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.24.75	Staphylococcus simulans	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.4.24.75	ammonium sulfate precipitation and Unosphere S column chromatography	Staphylococcus simulans

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.24.75	peptidoglycan + H2O	peptidoglycans from cell walls of Staphylococcus aureus and Staphylococcus haemolyticus	Staphylococcus simulans	fragments of peptidogylcan	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.4.24.75	?	x * 27500, SDS-PAGE	Staphylococcus simulans
3.4.24.75	?	x * 27517, calculated from amino acid sequence	Staphylococcus simulans

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
3.4.24.75	50	-	-	Staphylococcus simulans

General Information

EC Number	General Information	Comment	Organism
3.4.24.75	physiological function	the enzyme lyses staphylococcus cell walls by cleaving pentaglycine cross_bridges in their peptidoglycan	Staphylococcus simulans

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Release 2023.1 (January 2023)