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Literature summary extracted from

  • Shek, R.; Hilaire, T.; Sim, J.; French, J.B.
    Structural determinants for substrate selectivity in guanine deaminase enzymes of the amidohydrolase superfamily (2019), Biochemistry, 58, 3280-3292 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.3 expression in Escherichia coli Escherichia coli
3.5.4.3 expression in Escherichia coli Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.4.3 GuaD retains the overall TIM barrel tertiary structure conserved among amidohydrolase enzymes. GuaD also possesses a single zinc cation with trigonal bipyrimidal coordination geometry within its active site Escherichia coli
3.5.4.3 Gud1 retains the overall TIM barrel tertiary structure conserved among amidohydrolase enzymes. Gud1 also possesses a single zinc cation with trigonal bipyrimidal coordination geometry within its active site Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.3 A386S 4.8fold decrease in kcat/Km value Escherichia coli
3.5.4.3 D327L no residual activity Escherichia coli
3.5.4.3 E240D no residual activity Escherichia coli
3.5.4.3 F210A 20fold decrease in kcat/Km value Escherichia coli
3.5.4.3 H237R no residual activity Escherichia coli
3.5.4.3 L305A no residual activity Escherichia coli
3.5.4.3 Q87N no residual activity Escherichia coli
3.5.4.3 W101H 8.9fold decrease in kcat/Km value Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.3 0.061
-
 guanine wild-type, pH 7.5, 25°C Saccharomyces cerevisiae
3.5.4.3 0.08
-
 guanine mutant W101H, pH 7.5, 25°C Escherichia coli
3.5.4.3 0.14
-
 guanine mutant A386S, pH 7.5, 25°C Escherichia coli
3.5.4.3 0.21
-
 guanine wild-type, pH 7.5, 25°C Escherichia coli
3.5.4.3 0.21
-
 guanine mutant F210A, pH 7.5, 25°C Escherichia coli
3.5.4.3 0.93
-
 1-methylguanine wild-type, pH 7.5, 25°C Escherichia coli
3.5.4.3 1
-
 8-Azaguanine wild-type, pH 7.5, 25°C Escherichia coli
3.5.4.3 1.2
-
 ammeline wild-type, pH 7.5, 25°C Escherichia coli
3.5.4.3 2.6
-
 ammeline wild-type, pH 7.5, 25°C Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.3 Zinc GuaD possesses a single zinc cation with trigonal bipyrimidal coordination geometry within its active site Escherichia coli
3.5.4.3 Zinc Gud1 possesses a single zinc cation with trigonal bipyrimidal coordination geometry within its active site Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.3 Escherichia coli P76641
-
-
3.5.4.3 Saccharomyces cerevisiae Q07729
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.3 1-methylguanine + H2O
-
 Escherichia coli 1-methylxanthine + NH3
-
 ?
3.5.4.3 8-azaguanine + H2O
-
 Escherichia coli 8-azaxanthine + NH3
-
 ?
3.5.4.3 ammeline + H2O
-
 Escherichia coli ? + NH3
-
 ?
3.5.4.3 ammeline + H2O
-
 Saccharomyces cerevisiae ? + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Escherichia coli xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Saccharomyces cerevisiae xanthine + NH3
-
 ?
3.5.4.3 additional information 9-methylguanine, 6-O-methylguanine, 6-thioguanine, and 2,6-diaminopurine, also exhibit measurable activity but turn over very slowly, i.e. below 0.001 per s Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.3 guaD
-
 Escherichia coli
3.5.4.3 GUD1
-
 Saccharomyces cerevisiae
3.5.4.3 YgfP
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.4.3 0.08
-
 1-methylguanine wild-type, pH 7.5, 25°C Escherichia coli
3.5.4.3 0.13
-
 guanine mutant W101H, pH 7.5, 25°C Escherichia coli
3.5.4.3 0.14
-
 guanine mutant F210A, pH 7.5, 25°C Escherichia coli
3.5.4.3 0.39
-
 guanine mutant A386S, pH 7.5, 25°C Escherichia coli
3.5.4.3 1
-
 ammeline wild-type, pH 7.5, 25°C Escherichia coli
3.5.4.3 2.8
-
 guanine wild-type, pH 7.5, 25°C Escherichia coli
3.5.4.3 2.8
-
 ammeline wild-type, pH 7.5, 25°C Saccharomyces cerevisiae
3.5.4.3 7
-
 8-Azaguanine wild-type, pH 7.5, 25°C Escherichia coli
3.5.4.3 8.9
-
 guanine wild-type, pH 7.5, 25°C Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.4.3 0.086
-
 1-methylguanine wild-type, pH 7.5, 25°C Escherichia coli
3.5.4.3 0.7
-
 guanine mutant F210A, pH 7.5, 25°C Escherichia coli
3.5.4.3 0.83
-
 ammeline wild-type, pH 7.5, 25°C Escherichia coli
3.5.4.3 1.1
-
 ammeline wild-type, pH 7.5, 25°C Saccharomyces cerevisiae
3.5.4.3 1.5
-
 guanine mutant W101H, pH 7.5, 25°C Escherichia coli
3.5.4.3 2.8
-
 guanine mutant A386S, pH 7.5, 25°C Escherichia coli
3.5.4.3 7
-
 8-Azaguanine wild-type, pH 7.5, 25°C Escherichia coli
3.5.4.3 13
-
 guanine wild-type, pH 7.5, 25°C Escherichia coli
3.5.4.3 150
-
 guanine wild-type, pH 7.5, 25°C Saccharomyces cerevisiae