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Literature summary extracted from

  • Stefanidis, L.; Fusco, N.D.; Cooper, S.E.; Smith-Carpenter, J.E.; Alper, B.J.
    Molecular determinants of substrate specificity in human insulin-degrading enzyme (2018), Biochemistry, 57, 4903-4914 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.56
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.56 A140D specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg Homo sapiens
3.4.24.56 A140F specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg Homo sapiens
3.4.24.56 A140K specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg Homo sapiens
3.4.24.56 A140N specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg Homo sapiens
3.4.24.56 A140W specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg Homo sapiens
3.4.24.56 A140Y specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg Homo sapiens
3.4.24.56 E111Q specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg Homo sapiens
3.4.24.56 Y831A specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg Homo sapiens
3.4.24.56 Y831D specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg Homo sapiens
3.4.24.56 Y831F specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg Homo sapiens
3.4.24.56 Y831K specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg Homo sapiens
3.4.24.56 Y831N specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg Homo sapiens
3.4.24.56 Y831W specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.56 Zn2+ zinc metalloendopeptidase Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.56 110000
-
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.56 Homo sapiens P14735
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.56
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.56 (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.56 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.56 amyloid beta + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.56 insulin + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.56 additional information a functional requirement for active site residues F115, A140, F141, Y150, W199, F202, F820, and Y831 is established, and specific contributions of residue charge, size, and hydrophobicity to substrate binding, specificity, and proteolysis are demonstrated Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.56 IDE
-
 Homo sapiens
3.4.24.56 Insulin-degrading enzyme
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.56 0.088
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme W199K Homo sapiens
3.4.24.56 0.095
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme Y150K Homo sapiens
3.4.24.56 0.097
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme F202D Homo sapiens
3.4.24.56 0.097
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme F202K Homo sapiens
3.4.24.56 0.1
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme F141W Homo sapiens
3.4.24.56 0.108
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme F820Y Homo sapiens
3.4.24.56 0.123
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme F141N Homo sapiens
3.4.24.56 0.143
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme Y150W Homo sapiens
3.4.24.56 0.148
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme F202W Homo sapiens
3.4.24.56 0.16
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme F820W Homo sapiens
3.4.24.56 0.16
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme W199Y Homo sapiens
3.4.24.56 0.17
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme F202N Homo sapiens
3.4.24.56 0.177
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme W199N Homo sapiens
3.4.24.56 0.178
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme F141Y Homo sapiens
3.4.24.56 0.18
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme W199A Homo sapiens
3.4.24.56 0.198
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, wild-type enzyme Homo sapiens
3.4.24.56 0.2
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme F115Y Homo sapiens
3.4.24.56 0.22
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme F141A Homo sapiens
3.4.24.56 0.22
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme Y150F Homo sapiens
3.4.24.56 0.24
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme F202Y Homo sapiens
3.4.24.56 0.24
-
 (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme W199F Homo sapiens
3.4.24.56 0.29
-
 (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme F820Y Homo sapiens
3.4.24.56 0.298
-
 (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme Y150K Homo sapiens
3.4.24.56 0.33
-
 (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme 141A Homo sapiens
3.4.24.56 0.338
-
 (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme Y150F Homo sapiens
3.4.24.56 0.45
-
 (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme Y150W Homo sapiens
3.4.24.56 0.53
-
 (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme F202D Homo sapiens
3.4.24.56 0.57
-
 (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme W199Y Homo sapiens
3.4.24.56 0.57
-
 (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, wild-type enzyme Homo sapiens
3.4.24.56 0.625
-
 (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme 141W Homo sapiens
3.4.24.56 0.72
-
 (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme F115Y Homo sapiens
3.4.24.56 0.725
-
 (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme F115A Homo sapiens
3.4.24.56 0.73
-
 (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme F115W Homo sapiens
3.4.24.56 0.735
-
 (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme F202Y Homo sapiens
3.4.24.56 0.78
-
 (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme 141Y Homo sapiens
3.4.24.56 0.803
-
 (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme F202W Homo sapiens
3.4.24.56 0.85
-
 (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme F820W Homo sapiens
3.4.24.56 1.1
-
 (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH pH 7.3, 37°C, mutant enzyme Y150F Homo sapiens