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Literature summary extracted from

  • Puorger, C.; Di Girolamo, S.; Lipps, G.
    Elucidation of the recognition sequence of sortase B from Bacillus anthracis by using a newly developed liquid chromatography-mass spectrometry-based method (2017), Biochemistry, 56, 2641-2650 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.22.71 analysis method for sequence specificity determination of sortases or other bond-forming enzymes that recognize an amino acid sequence. Using mixtures of recognition peptides of limited complexity, which are reacted with biotinylated substrates, the biotinylated transpeptidation products are isolated with magnetic streptavidin beads and analyzed via liquid chromatography and mass spectrometry Bacillus anthracis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.71 expression in Escherichia coli Bacillus anthracis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.71 Ca2+ about 2fold stimulation Bacillus anthracis
3.4.22.71 Mg2+ about 2fold stimulation Bacillus anthracis

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.71 Bacillus anthracis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.71 DNPNTGDE + H2O
-
 Bacillus anthracis ?
-
 ?
3.4.22.71 IsdC + H2O IsdC is a surface protein necessary for heme iron uptake in Bacillus anthracis. SrtB recognizes the NPKTG sequence present Bacillus anthracis ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.71 8 9
-
 Bacillus anthracis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.71 5
-
 no residual activity Bacillus anthracis