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Literature summary extracted from

  • Ohnishi, A.; Watanabe, J.; Ogawa, Y.; Goto, Y.; Hattori, A.; Tsujimoto, M.
    Involvement of phenylalanine 297 in the construction of the substrate pocket of human aminopeptidase B (2015), Biochemistry, 54, 6062-6070 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.11.6 NaCl the enzyme requires a physiological concentration of NaCl (150 mM) for optimal activity Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.6 expressed in Escherichia coli JM109 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.6 F297A the mutant shows no activity with kallidin or [Arg0]-Met-enkephalin Homo sapiens
3.4.11.6 F297Y the mutation causes a significant decrease in hydrolytic activity toward synthetic and peptide substrates (13fold and 15fold lower catalytic efficiencies with L-Arg-7-amido-4-methylcoumarin and L-Lys-7-amido-4-methylcoumarin, respectively. No activity with kallidin or [Arg0]-Met-enkephalin) as well as chloride anion sensitivity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.6 arphamenine A
-
 Homo sapiens
3.4.11.6 Arphamenine B
-
 Homo sapiens
3.4.11.6 bestatin
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.6 0.0529
-
 L-Arg-7-amido-4-methylcoumarin wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.4.11.6 0.208
-
 L-Arg-7-amido-4-methylcoumarin mutant enzyme F297Y, at pH 7.4 and 37°C Homo sapiens
3.4.11.6 0.323
-
 L-Lys-7-amido-4-methylcoumarin wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.4.11.6 0.598
-
 L-Lys-7-amido-4-methylcoumarin mutant enzyme F297Y, at pH 7.4 and 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.6 Homo sapiens Q9H4A4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.6 DEAE Toyopearl column chromatography and TALON column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.6 kallidin + H2O i.e. KRPPGFSPFR Homo sapiens bradykinin + ?
-
 ?
3.4.11.6 L-Ala-7-amido-4-methylcoumarin + H2O about 2% activity compared to L-Arg-7-amido-4-methylcoumarin Homo sapiens L-Ala + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Arg-7-amido-4-methylcoumarin + H2O 100% activity Homo sapiens L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Gln-7-amido-4-methylcoumarin + H2O about 1% activity compared to L-Arg-7-amido-4-methylcoumarin Homo sapiens L-Gln + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Ile-7-amido-4-methylcoumarin + H2O about 1% activity compared to L-Arg-7-amido-4-methylcoumarin Homo sapiens L-Ile + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Leu-7-amido-4-methylcoumarin + H2O about 1% activity compared to L-Arg-7-amido-4-methylcoumarin Homo sapiens L-Leu + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Lys-7-amido-4-methylcoumarin + H2O about 45% activity compared to L-Arg-7-amido-4-methylcoumarin Homo sapiens L-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Met-7-amido-4-methylcoumarin + H2O about 1% activity compared to L-Arg-7-amido-4-methylcoumarin Homo sapiens L-Met + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Phe-7-amido-4-methylcoumarin + H2O about 5% activity compared to L-Arg-7-amido-4-methylcoumarin Homo sapiens L-Phe + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Tyr-7-amido-4-methylcoumarin + H2O about 7% activity compared to L-Arg-7-amido-4-methylcoumarin Homo sapiens L-Tyr + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Val-7-amido-4-methylcoumarin + H2O about 1% activity compared to L-Arg-7-amido-4-methylcoumarin Homo sapiens L-Val + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 additional information no activity with L-Cys-7-amido-4-methylcoumarin, L-Asp-7-amido-4-methylcoumarin, and L-Glu-7-amido-4-methylcoumarin Homo sapiens ?
-
 ?
3.4.11.6 [Arg0]-Met-enkephalin + H2O i.e. RYGGFM Homo sapiens Met-enkephalin + de-[Tyr]-Met-enkephalin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.6 ? x * 74000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.11.6 APB
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.6 1.56
-
 L-Lys-7-amido-4-methylcoumarin mutant enzyme F297Y, at pH 7.4 and 37°C Homo sapiens
3.4.11.6 2.95
-
 L-Arg-7-amido-4-methylcoumarin mutant enzyme F297Y, at pH 7.4 and 37°C Homo sapiens
3.4.11.6 9.64
-
 L-Arg-7-amido-4-methylcoumarin wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.4.11.6 12.9
-
 L-Lys-7-amido-4-methylcoumarin wild type enzyme, at pH 7.4 and 37°C Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.11.6 0.0000007
-
 wild type enzyme, at pH 7.4 and 37°C Homo sapiens Arphamenine B
3.4.11.6 0.00000382
-
 wild type enzyme, at pH 7.4 and 37°C Homo sapiens bestatin
3.4.11.6 0.00000402
-
 wild type enzyme, at pH 7.4 and 37°C Homo sapiens arphamenine A

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.11.6 2.61
-
 L-Lys-7-amido-4-methylcoumarin mutant enzyme F297Y, at pH 7.4 and 37°C Homo sapiens
3.4.11.6 14.2
-
 L-Arg-7-amido-4-methylcoumarin mutant enzyme F297Y, at pH 7.4 and 37°C Homo sapiens
3.4.11.6 39.9
-
 L-Lys-7-amido-4-methylcoumarin wild type enzyme, at pH 7.4 and 37°C Homo sapiens
3.4.11.6 182
-
 L-Arg-7-amido-4-methylcoumarin wild type enzyme, at pH 7.4 and 37°C Homo sapiens