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Literature summary extracted from

  • Luka, Z.; Moss, F.; Loukachevitch, L.V.; Bornhop, D.J.; Wagner, C.
    Histone demethylase LSD1 is a folate-binding protein (2011), Biochemistry, 50, 4750-4756 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.65 tetrahydrofolate binding kinetic analysis of folate derivatives, e.g. folates bound to glutamates, the (6R,S) form of the natural pentaglutamate form of tetrahydrofolate bound with the highest affinity to full-length LSD1, binding of different forms of folate to LSD1, and binding structures, overview Homo sapiens
1.14.99.66 tetrahydrofolate binding kinetic analysis of folate derivatives, e.g. folates bound to glutamates, the (6R,S) form of the natural pentaglutamate form of tetrahydrofolate bound with the highest affinity to full-length LSD1, binding of different forms of folate to LSD1, and binding structures, overview Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.65 recombinant expression of N-terminally His-tagged enzyme in Escherichia coli BL21(DE3), recombinant expression of N-terminally truncated GST-tagged LSD1 Homo sapiens
1.14.99.66 recombinant expression of N-terminally His-tagged enzyme in Escherichia coli BL21(DE3), recombinant expression of N-terminally truncated GST-tagged LSD1 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.11.65 nucleus LSD1 is associated with folate Homo sapiens 5634
-
1.14.99.66 nucleus LSD1 is associated with folate Homo sapiens 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.65 100000
-
about, recombinant enzyme, gel filtration Homo sapiens
1.14.99.66 100000
-
about, recombinant enzyme, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.65 [histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2 Homo sapiens
-
[histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2
-
?
1.14.11.65 [histone H3]-N6-methyl-L-lysine 9 + 2-oxoglutarate + O2 Homo sapiens
-
[histone H3]-L-lysine 9 + succinate + formaldehyde + CO2
-
?
1.14.99.66 [histone H3]-N6,N6-dimethyl-L-lysine 4 + 2-oxoglutarate + O2 Homo sapiens
-
[histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2
-
?
1.14.99.66 [histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2 Homo sapiens
-
[histone H3]-L-lysine 4 + succinate + formaldehyde + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.65 Homo sapiens O60341
-
-
1.14.99.66 Homo sapiens O60341
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.65 recombinant N-terminally His-tagged enzyme by nickel affinity chromatography from Escherichia coli BL21(DE3), or by ammonium sulfate fractionation and anion exchange chromatography, recombinant N-terminally truncated GST-tagged LSD1 by glutathione affinity chromatography Homo sapiens
1.14.99.66 recombinant N-terminally His-tagged enzyme by nickel affinity chromatography from Escherichia coli BL21(DE3), or by ammonium sulfate fractionation and anion exchange chromatography, recombinant N-terminally truncated GST-tagged LSD1 by glutathione affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.65 HeLa cell nuclear extracts of HeLa cells contain LSD1 that is associated with folate Homo sapiens
-
1.14.99.66 HeLa cell nuclear extracts of HeLa cells contain LSD1 that is associated with folate Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.65 additional information the bifunctional enzyme catalyzes the demethylation of H3K9me2/me1 and H3K4me2/me1 (1.14.99.66) Homo sapiens ?
-
?
1.14.11.65 [histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2
-
Homo sapiens [histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2
-
?
1.14.11.65 [histone H3]-N6-methyl-L-lysine 9 + 2-oxoglutarate + O2
-
Homo sapiens [histone H3]-L-lysine 9 + succinate + formaldehyde + CO2
-
?
1.14.99.66 additional information the bifunctional enzyme catalyzes the demethylation of H3K9me2/me1 (EC 1.14.11.65) and H3K4me2/me1 Homo sapiens ?
-
?
1.14.99.66 [histone H3]-N6,N6-dimethyl-L-lysine 4 + 2-oxoglutarate + O2
-
Homo sapiens [histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2
-
?
1.14.99.66 [histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2
-
Homo sapiens [histone H3]-L-lysine 4 + succinate + formaldehyde + CO2
-
?

Subunits

EC Number Subunits Comment Organism
1.14.11.65 monomer 1 * 95059, LSD1 without N-terminal methionine, mass spectrometry Homo sapiens
1.14.99.66 monomer 1 * 95059, LSD1 without N-terminal methionine, mass spectrometry Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.14.11.65 LSD1
-
Homo sapiens
1.14.11.65 More see also for EC 1.14.99.66 Homo sapiens
1.14.99.66 LSD1
-
Homo sapiens
1.14.99.66 More see also for EC 1.14.11.65 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.65 22
-
assay at room temperature Homo sapiens
1.14.99.66 22
-
assay at room temperature Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.65 7.2 7.5 assay at Homo sapiens
1.14.99.66 7.2 7.5 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.65 FAD required, a flavin-containing enzyme Homo sapiens
1.14.99.66 FAD required, a flavin-containing enzyme Homo sapiens

General Information

EC Number General Information Comment Organism
1.14.11.65 additional information mechanism of histone H3 demethylation by demethylase LSD1, overview Homo sapiens
1.14.11.65 physiological function methylation on the N-terminal tails of histone lysines serves as an epigenetic control mechanism, which is regulated by demathylases Homo sapiens
1.14.99.66 additional information mechanism of histone H3 demethylation by demethylase LSD1, overview Homo sapiens
1.14.99.66 physiological function methylation on the N-terminal tails of histone lysines serves as an epigenetic control mechanism, which is regulated by demathylases Homo sapiens