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Literature summary extracted from

  • Aeed, P.A.; Sperry, A.E.; Young, C.L.; Nagiec, M.M.; Elhammer, A.P.
    Effect of membrane perturbants on the activity and phase distribution of inositol phosphorylceramide synthase; development of a novel assay (2004), Biochemistry, 43, 8483-8493 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.227 analysis assays using CHAPS- or taurodeoxycholate-washed membranes are reproducible, completely dependent on added acceptor substrate C6-7-nitro-2-1,3-benzoxadiazol-4-yl-ceramide, and more than 95% dependent on added donor substrate phosphatidylinositol. Product formation is linear with respect to both enzyme concentration and time, and transfer efficiency is improved more than 20fold as compared to assays using crude membranes Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.227 1-phosphatidyl-1D-myo-inositol substrate inhibition Candida albicans
2.7.1.227 amidosulfobetaine-16 inactivates the enzyme irreversibly Candida albicans
2.7.1.227 Brij inactivates the enzyme Candida albicans
2.7.1.227 Empigen BB inactivates the enzyme irreversibly Candida albicans
2.7.1.227 N-dodecyl-N,N-(dimethylammonio)butanoate inactivates the enzyme irreversibly Candida albicans
2.7.1.227 Nonidet P-40 inactivates the enzyme Candida albicans
2.7.1.227 octylglucoside inactivates the enzyme Candida albicans
2.7.1.227 Triton X-100 inactivates the enzyme Candida albicans
2.7.1.227 Tween 20 inactivates the enzyme Candida albicans
2.7.1.227 Tween 80 inactivates the enzyme Candida albicans
2.7.1.227 Zwittergent 3-10 inactivates the enzyme irreversibly Candida albicans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.227 0.0033
-
6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-hexanoyl)-sphingosine CHAPS-washed membranes, pH 7.5, 23°C Candida albicans
2.7.1.227 0.138
-
1-phosphatidyl-1D-myo-inositol CHAPS-washed membranes, pH 7.5, 23°C Candida albicans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.227 membrane
-
Candida albicans 16020
-

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.227 Candida albicans O13332
-
-
2.7.1.227 Candida albicans ATCC MYA-2876 O13332
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.227 detergents Triton X-100, Nonidet P-40, Brij, Tween20, Tween 80 and octylglucoside all inactivate the enzyme. At moderate concentrations, the activity of the Triton X-100- and octylglucoside-solubilized material can be partially restored by inclusion of 5 mM phosphatidylinositol in the solubilization buffer. Detergents Empigen BB, N-dodecyl-N,N-(dimethylammonio)butanoate, Zwittergent 3-10, and amidosulfobetaine-16 all inactivate the enzyme essentially irreversibly. IPC synthase activity in membranes remaines largely intact and sedimentable at CHAPS concentrations of 4% where more than 90% of the phospholipids and 60% of the total proteins are extracted from the membranes Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.227 1-phosphatidyl-1D-myo-inositol + 6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-hexanoyl)-sphingosine
-
Candida albicans ?
-
?
2.7.1.227 1-phosphatidyl-1D-myo-inositol + 6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-hexanoyl)-sphingosine
-
Candida albicans ATCC MYA-2876 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.227 AUR1
-
Candida albicans

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.227 0.588
-
CHAPS-washed membranes, pH 7.5, 23°C Candida albicans 1-phosphatidyl-1D-myo-inositol