Any feedback?
Literature summary extracted from
- Molecular cloning, biochemical characterization, and antitumor properties of a novel L-asparaginase from Synechococcus elongatus PCC6803 (2016), Biochemistry (Moscow), 81, 1173-1181 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.5.1.1 | medicine | enzyme shows antitumor proterties with IC50 values 0.036 mg/ml for MCF-7 cells, 0.037 mM/ml for HCT-116 cells, 0.046 mg/ml for Hep-G2 cells, respectively | Synechococcus elongatus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.1 | expression in Escherichia coli | Synechococcus elongatus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.1 | Synechococcus elongatus | - |
- |
- |
| 3.5.1.1 | Synechococcus elongatus PCC 6803 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.1 | recombinant potein | Synechococcus elongatus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.1 | L-asparagine + H2O | - |
Synechococcus elongatus | L-aspartate + NH3 | - |
? |  |
| 3.5.1.1 | L-asparagine + H2O | - |
Synechococcus elongatus PCC 6803 | L-aspartate + NH3 | - |
? | |
| 3.5.1.1 | additional information | less than 0.5% of the activity with L-asparagine: L-glutamine, D-asparagine, D-glutamine | Synechococcus elongatus | ? | - |
? | |
| 3.5.1.1 | additional information | less than 0.5% of the activity with L-asparagine: L-glutamine, D-asparagine, D-glutamine | Synechococcus elongatus PCC 6803 | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 37 | - |
- |
Synechococcus elongatus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 45 | - |
about 25% of maximum activity | Synechococcus elongatus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 7.5 | - |
- |
Synechococcus elongatus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 8 | - |
about 75% of maximum activity | Synechococcus elongatus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
