EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.3.12 | expression in Escherichia coli BL21(DE3) | Listeria monocytogenes serotype 1/2a |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.3.12 | 2-chloroacetamidine | - |
Listeria monocytogenes serotype 1/2a | |
3.5.3.12 | iodoacetamide | - |
Listeria monocytogenes serotype 1/2a | |
3.5.3.12 | N-(4-aminobutyl)-2-chloro-ethanimidamide | - |
Listeria monocytogenes serotype 1/2a | |
3.5.3.12 | N-(4-aminobutyl)-2-fluoro-ethanimidamide | - |
Listeria monocytogenes serotype 1/2a |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.3.12 | 0.33 | - |
agmatine | pH 7.5, 25°C | Listeria monocytogenes serotype 1/2a |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.3.12 | agmatine + H2O | Listeria monocytogenes serotype 1/2a | the ability of Listeria monocytogenes to survive in extreme environments (i.e. low temperatures and pH) may arise, at least in part, from agmatine catabolism via the agmatine deiminase system. This catabolic pathway utilizes an agmatine deiminase to hydrolyse agmatine into N-carbamoylputrescine, with concomitant release of ammonia, which increases the pH, thus mitigating the ill effects of the acidic environment | N-carbamoylputrescine + NH3 | - |
? | |
3.5.3.12 | agmatine + H2O | Listeria monocytogenes serotype 1/2a 10403S | the ability of Listeria monocytogenes to survive in extreme environments (i.e. low temperatures and pH) may arise, at least in part, from agmatine catabolism via the agmatine deiminase system. This catabolic pathway utilizes an agmatine deiminase to hydrolyse agmatine into N-carbamoylputrescine, with concomitant release of ammonia, which increases the pH, thus mitigating the ill effects of the acidic environment | N-carbamoylputrescine + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.3.12 | Listeria monocytogenes serotype 1/2a | A0A0H3G8U9 | - |
- |
3.5.3.12 | Listeria monocytogenes serotype 1/2a 10403S | A0A0H3G8U9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.3.12 | - |
Listeria monocytogenes serotype 1/2a |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.3.12 | agmatine + H2O | - |
Listeria monocytogenes serotype 1/2a | N-carbamoylputrescine + NH3 | - |
? | |
3.5.3.12 | agmatine + H2O | the ability of Listeria monocytogenes to survive in extreme environments (i.e. low temperatures and pH) may arise, at least in part, from agmatine catabolism via the agmatine deiminase system. This catabolic pathway utilizes an agmatine deiminase to hydrolyse agmatine into N-carbamoylputrescine, with concomitant release of ammonia, which increases the pH, thus mitigating the ill effects of the acidic environment | Listeria monocytogenes serotype 1/2a | N-carbamoylputrescine + NH3 | - |
? | |
3.5.3.12 | agmatine + H2O | - |
Listeria monocytogenes serotype 1/2a 10403S | N-carbamoylputrescine + NH3 | - |
? | |
3.5.3.12 | agmatine + H2O | the ability of Listeria monocytogenes to survive in extreme environments (i.e. low temperatures and pH) may arise, at least in part, from agmatine catabolism via the agmatine deiminase system. This catabolic pathway utilizes an agmatine deiminase to hydrolyse agmatine into N-carbamoylputrescine, with concomitant release of ammonia, which increases the pH, thus mitigating the ill effects of the acidic environment | Listeria monocytogenes serotype 1/2a 10403S | N-carbamoylputrescine + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.3.12 | ? | x * 46800, SDS-PAGE | Listeria monocytogenes serotype 1/2a |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.3.12 | AgD | - |
Listeria monocytogenes serotype 1/2a |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.3.12 | 0.517 | - |
agmatine | pH 7.5, 25°C | Listeria monocytogenes serotype 1/2a |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.3.12 | 7.5 | - |
- |
Listeria monocytogenes serotype 1/2a |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.3.12 | 5.5 | 9.5 | pH 5.5: about 60% of maximal activity, pH 9.5: about 65% of maximal activity | Listeria monocytogenes serotype 1/2a |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.5.3.12 | 0.0785 | - |
pH 7.5, 25°C | Listeria monocytogenes serotype 1/2a | N-(4-aminobutyl)-2-chloro-ethanimidamide | |
3.5.3.12 | 0.146 | - |
pH 7.5, 25°C | Listeria monocytogenes serotype 1/2a | N-(4-aminobutyl)-2-fluoro-ethanimidamide | |
3.5.3.12 | 221 | - |
pH 7.5, 25°C | Listeria monocytogenes serotype 1/2a | 2-chloroacetamidine |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.5.3.12 | metabolism | the ability of Listeria monocytogenes to survive in extreme environments (i.e. low temperatures and pH) may arise, at least in part, from agmatine catabolism via the agmatine deiminase system. This catabolic pathway utilizes an agmatine deiminase to hydrolyse agmatine into N-carbamoylputrescine, with concomitant release of ammonia, which increases the pH, thus mitigating the ill effects of the acidic environment | Listeria monocytogenes serotype 1/2a |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.3.12 | 1.57 | - |
agmatine | pH 7.5, 25°C | Listeria monocytogenes serotype 1/2a |